LOCUS BBa_J18901 3071 bp DNA linear 25-Nov-2024 DEFINITION pSB1AC3F construction plasmid for protein fusion BioBricks ACCESSION BBa_J18901 VERSION BBa_J18901.1 FEATURES Location/Qualifiers misc_feature 1..30 /label=extended BioBrick suffix misc_feature 3043..3071 /label=extended BioBrick prefix primer_bind 2924..2944 /label=BBVF2 primer_bind 166..185 /label=BBVR (G00101) CDS 1942..2802 /label=AmpR CDS 1173..1830 /label=ChlR misc_feature 270..883 /label=Replication Origin (MB1) ORIGIN 1 accggttaat actagtagcg gccgctgcag tccggcaaaa aaacgggcaa ggtgtcacca 61 ccctgccctt tttctttaaa accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc 121 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 181 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 241 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 301 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 361 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 421 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 481 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 541 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 601 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 661 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 721 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 781 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 841 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 901 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 961 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 1021 tatgagtaaa cttggtctga cagctcgagg cttggattct caccaataaa aaacgcccgg 1081 cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc 1141 aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact catcgcagta 1201 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac 1261 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa 1321 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac 1381 ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag 1441 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc 1501 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca 1561 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac gaaattccgg 1621 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt 1681 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca 1741 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac 1801 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga 1861 taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct 1921 tacgtgcccg atcaactcga gttaccaatg cttaatcagt gaggcaccta tctcagcgat 1981 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 2041 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 2101 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 2161 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 2221 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 2281 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 2341 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 2401 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 2461 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 2521 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 2581 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 2641 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 2701 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 2761 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 2821 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 2881 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 2941 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggcagaattt 3001 cagataaaaa aaatccttag ctttcgctaa ggatgatttc tggaattcgc ggccgcttct 3061 agatggccgg c //