LOCUS       BBa_J18902              3205 bp    DNA     linear       25-Nov-2024
DEFINITION  pSB1AK3F construction plasmid for protein fusion BioBricks
ACCESSION   BBa_J18902
VERSION     BBa_J18902.1  
FEATURES             Location/Qualifiers
     misc_feature    1..30
                     /label=extended BioBrick suffix
     misc_feature    3177..3205
                     /label=extended BioBrick prefix
     CDS             1106..1921
                     /label=KanR
     CDS             2076..2936
                     /label=AmpR
     primer_bind     3058..3078
                     /label=BBF2
     primer_bind     166..185
                     /label=BBVR
     misc_feature    270..883
                     /label=Replication Origin (MB1)
ORIGIN
        1 accggttaat actagtagcg gccgctgcag tccggcaaaa aaacgggcaa ggtgtcacca
       61 ccctgccctt tttctttaaa accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc
      121 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
      181 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
      241 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
      301 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
      361 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
      421 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
      481 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
      541 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
      601 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
      661 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
      721 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
      781 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
      841 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
      901 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
      961 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     1021 tatgagtaaa cttggtctga cagctcgagt cccgtcaagt cagcgtaatg ctctgccagt
     1081 gttacaacca attaaccaat tctgattaga aaaactcatc gagcatcaaa tgaaactgca
     1141 atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag
     1201 gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc
     1261 cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa
     1321 gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagc ttatgcattt
     1381 ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa
     1441 ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgctgttaa
     1501 aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa
     1561 caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt ttcccgggga
     1621 tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa
     1681 gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa
     1741 cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcgat
     1801 agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag
     1861 catccatgtt ggaatttaat cgcggcctgg agcaagacgt ttcccgttga atatggctca
     1921 taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat
     1981 ttttatcttg tgcaatgtaa catcagagat tttgagacac aacgtggctt tgttgaataa
     2041 atcgaacttt tgctgagttg aaggatcagc tcgagttacc aatgcttaat cagtgaggca
     2101 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     2161 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     2221 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     2281 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     2341 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     2401 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     2461 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     2521 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     2581 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     2641 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     2701 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     2761 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     2821 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     2881 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     2941 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     3001 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     3061 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     3121 acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat
     3181 tcgcggccgc ttctagatgg ccggc
//