LOCUS BBa_J18903 3462 bp DNA linear 22-Dec-2024 DEFINITION pSB1AT3F construction plasmid for protein fusion BioBricks ACCESSION BBa_J18903 VERSION BBa_J18903.1 FEATURES Location/Qualifiers misc_feature 1..30 /label=extended BioBrick suffix misc_feature 3434..3462 /label=extended BioBrick prefix primer_bind 166..185 /label=BBVR primer_bind 3315..3335 /label=BBVF2 CDS 2333..3193 /label=AmpR CDS 1136..2326 /label=TetR misc_feature 270..884 /label=replication origin (MB1) ORIGIN 1 accggttaat actagtagcg gccgctgcag tccggcaaaa aaacgggcaa ggtgtcacca 61 ccctgccctt tttctttaaa accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc 121 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 181 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 241 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 301 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 361 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 421 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 481 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 541 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 601 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 661 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 721 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 781 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 841 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 901 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 961 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 1021 tatgagtaaa cttggtctga cagctcgaga ttctcatgtt tgacagctta tcatcgataa 1081 gctttaatgc ggtagtttat cacagttaaa ttgctaacgc agtcaggcac cgtgtatgaa 1141 atctaacaat gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg taggcatagg 1201 cttggttatg ccggtactgc cgggcctctt gcgggatatc gtccattccg acagcatcgc 1261 cagtcactat ggcgtgctgc tagcgctata tgcgttgatg caatttctat gcgcacccgt 1321 tctcggagca ctgtccgacc gctttggccg ccgcccagtc ctgctcgctt cgctacttgg 1381 agccactatc gactacgcga tcatggcgac cacacccgtc ctgtggatcc tctacgccgg 1441 acgcatcgtg gccggtatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga 1501 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt 1561 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc 1621 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca 1681 ggagtcgcat aagggagagc gtcgaccgat gcccttgaga gccttcaacc cagtcagctc 1741 cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat 1801 gcaactcgta ggacaggttc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg 1861 ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc 1921 tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc 1981 gggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat 2041 ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc 2101 catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat cgctcgcggc 2161 tcttaccagc ctaacttcga tcactggacc gctgatcgtc acggcgattt atgccgcctc 2221 ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc ttgtctgcct 2281 ccccgcgttg cgtcgcggtg catggagccg ggccacctcg acctaactcg agttaccaat 2341 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 2401 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 2461 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 2521 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 2581 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 2641 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 2701 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 2761 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 2821 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 2881 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 2941 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 3001 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 3061 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 3121 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 3181 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 3241 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 3301 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct 3361 ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta gctttcgcta 3421 aggatgattt ctggaattcg cggccgcttc tagatggccg gc //