BBa_J18912
1
T7start
T7 promoter + RBS + START
2010-01-26T12:00:00Z
2015-08-31T04:08:36Z
pET3a expression vector
Strong T7 promoter and RBS including start codon for protein expression in E. coli or in-vitro.
Part is provided in RFC 25 assembly format.
false
false
_165_
0
2175
165
It's complicated
false
* sequence is taken from the commonly used pET3a expression vector
* deleted XbaI site between prom. and RBS
* T7 promoter sequence (1-19) equals the one in BBa_I712074
* Conservative choice of part boundaries and modifications -- the part's size could probably be reduced.
false
Raik Gruenberg
annotation2065586
1
start
range2065586
1
81
83
BBa_J18912_sequence
1
taatacgactcactatagggagaccacaacggtttccctctagcaataattttgtttaactttaagaaggagatatacatatg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z