BBa_J18938
type: ComponentDefinition
persistent identity: BBa J18938
display id: BBa_J18938
version: 1
was generated by: iGEM to SBOL conversion
description: FKBP ~ bla-frag1
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: synthetic
owned by: james
owned by: myers
mutable description: P06 expression cassette from Gruenberg et al. 2010 series of interaction reporter proteins. All proteins share the same architecture: * T7Start-domain1-5xGS-domain2-preSCsite-HisTag-T7Stop Only Domains1 and 2 vary and are taken from a list of protein interaction input and output (readout) parts. ===Expression=== Proteins were expressed in E. coli BL21 using T7 Polymerase and were purified by His-tag affinity. ===Reference=== * Gruenberg, Ferrar, van der Sloot, Serrano (2010): Building Blocks for Protein Interaction Devices
discontinued: false
dominant: false
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructs were assembled according to R.F.C. 25. All parts were codon optimized for E. coli.
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
component: BBa_J18922
component: BBa_B0105
component: BBa_I757011
component: BBa_J18919
component: BBa_J18909
component: BBa_J18913
component: BBa_J18912
component: BBa_J18925
sequence annotation: BBa_J18912
sequence annotation: BBa_B0105
sequence annotation: BBa_J18925
sequence annotation: BBa_J18922
sequence annotation: BBa_I757011
sequence annotation: BBa_J18919
sequence annotation: BBa_J18909
sequence annotation: BBa_J18913
sequence: https://synbiohub.org/public/igem/BBa_J18938_sequence/1

BBa_J18922
type: Component
persistent identity: component2066493
display id: component2066493
version: 1
top level: BBa_J18938
definition: 5xGS
access: public

BBa_B0105
type: Component
persistent identity: component2066492
display id: component2066492
version: 1
top level: BBa_J18938
definition: Scar 25
access: public

BBa_I757011
type: Component
persistent identity: component2066499
display id: component2066499
version: 1
top level: BBa_J18938
definition: BBa_I757011
access: public

BBa_B0105
type: Component
persistent identity: component2066495
display id: component2066495
version: 1
top level: BBa_J18938
definition: Scar 25
access: public

BBa_J18919
type: Component
persistent identity: component2066502
display id: component2066502
version: 1
top level: BBa_J18938
definition: preSCsite
access: public

BBa_B0105
type: Component
persistent identity: component2066501
display id: component2066501
version: 1
top level: BBa_J18938
definition: Scar 25
access: public

BBa_J18909
type: Component
persistent identity: component2066506
display id: component2066506
version: 1
top level: BBa_J18938
definition: HisTag
access: public

BBa_B0105
type: Component
persistent identity: component2066504
display id: component2066504
version: 1
top level: BBa_J18938
definition: Scar 25
access: public

BBa_J18913
type: Component
persistent identity: component2066510
display id: component2066510
version: 1
top level: BBa_J18938
definition: T7stop
access: public

BBa_B0105
type: Component
persistent identity: component2066508
display id: component2066508
version: 1
top level: BBa_J18938
definition: Scar 25
access: public

BBa_J18912
type: Component
persistent identity: component2066487
display id: component2066487
version: 1
top level: BBa_J18938
definition: T7start
access: public

BBa_J18925
type: Component
persistent identity: component2066490
display id: component2066490
version: 1
top level: BBa_J18938
definition: FKBP12
access: public

BBa_B0105
type: Component
persistent identity: component2066489
display id: component2066489
version: 1
top level: BBa_J18938
definition: Scar 25
access: public

BBa_J18912
type: SequenceAnnotation
persistent identity: annotation2066487
display id: annotation2066487
version: 1
top level: BBa_J18938
location: https://synbiohub.org/public/igem/BBa_J18938/annotation2066487/range2066487/1
component: BBa_J18912

https://synbiohub.org/public/igem/BBa_J18938/annotation2066487/range2066487/1
type: Range
persistent identity: range2066487
display id: range2066487
version: 1
top level: BBa_J18938
direction: direction/reverse
start: 1
end: 83
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066489
display id: annotation2066489
version: 1
top level: BBa_J18938
location: https://synbiohub.org/public/igem/BBa_J18938/annotation2066489/range2066489/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18938/annotation2066489/range2066489/1
type: Range
persistent identity: range2066489
display id: range2066489
version: 1
top level: BBa_J18938
direction: direction/reverse
start: 84
end: 89
orientation: inline

BBa_J18925
type: SequenceAnnotation
persistent identity: annotation2066490
display id: annotation2066490
version: 1
top level: BBa_J18938
location: https://synbiohub.org/public/igem/BBa_J18938/annotation2066490/range2066490/1
component: BBa_J18925

https://synbiohub.org/public/igem/BBa_J18938/annotation2066490/range2066490/1
type: Range
persistent identity: range2066490
display id: range2066490
version: 1
top level: BBa_J18938
direction: direction/reverse
start: 90
end: 410
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066492
display id: annotation2066492
version: 1
top level: BBa_J18938
location: https://synbiohub.org/public/igem/BBa_J18938/annotation2066492/range2066492/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18938/annotation2066492/range2066492/1
type: Range
persistent identity: range2066492
display id: range2066492
version: 1
top level: BBa_J18938
direction: direction/reverse
start: 411
end: 416
orientation: inline

BBa_J18922
type: SequenceAnnotation
persistent identity: annotation2066493
display id: annotation2066493
version: 1
top level: BBa_J18938
location: https://synbiohub.org/public/igem/BBa_J18938/annotation2066493/range2066493/1
component: BBa_J18922

https://synbiohub.org/public/igem/BBa_J18938/annotation2066493/range2066493/1
type: Range
persistent identity: range2066493
display id: range2066493
version: 1
top level: BBa_J18938
direction: direction/reverse
start: 417
end: 446
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066495
display id: annotation2066495
version: 1
top level: BBa_J18938
location: https://synbiohub.org/public/igem/BBa_J18938/annotation2066495/range2066495/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18938/annotation2066495/range2066495/1
type: Range
persistent identity: range2066495
display id: range2066495
version: 1
top level: BBa_J18938
direction: direction/reverse
start: 447
end: 452
orientation: inline

BBa_I757011
type: SequenceAnnotation
persistent identity: annotation2066499
display id: annotation2066499
version: 1
top level: BBa_J18938
location: https://synbiohub.org/public/igem/BBa_J18938/annotation2066499/range2066499/1
component: BBa_I757011

https://synbiohub.org/public/igem/BBa_J18938/annotation2066499/range2066499/1
type: Range
persistent identity: range2066499
display id: range2066499
version: 1
top level: BBa_J18938
direction: direction/reverse
start: 453
end: 995
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066501
display id: annotation2066501
version: 1
top level: BBa_J18938
location: https://synbiohub.org/public/igem/BBa_J18938/annotation2066501/range2066501/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18938/annotation2066501/range2066501/1
type: Range
persistent identity: range2066501
display id: range2066501
version: 1
top level: BBa_J18938
direction: direction/reverse
start: 996
end: 1001
orientation: inline

BBa_J18919
type: SequenceAnnotation
persistent identity: annotation2066502
display id: annotation2066502
version: 1
top level: BBa_J18938
location: https://synbiohub.org/public/igem/BBa_J18938/annotation2066502/range2066502/1
component: BBa_J18919

https://synbiohub.org/public/igem/BBa_J18938/annotation2066502/range2066502/1
type: Range
persistent identity: range2066502
display id: range2066502
version: 1
top level: BBa_J18938
direction: direction/reverse
start: 1002
end: 1025
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066504
display id: annotation2066504
version: 1
top level: BBa_J18938
location: https://synbiohub.org/public/igem/BBa_J18938/annotation2066504/range2066504/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18938/annotation2066504/range2066504/1
type: Range
persistent identity: range2066504
display id: range2066504
version: 1
top level: BBa_J18938
direction: direction/reverse
start: 1026
end: 1031
orientation: inline

BBa_J18909
type: SequenceAnnotation
persistent identity: annotation2066506
display id: annotation2066506
version: 1
top level: BBa_J18938
location: https://synbiohub.org/public/igem/BBa_J18938/annotation2066506/range2066506/1
component: BBa_J18909

https://synbiohub.org/public/igem/BBa_J18938/annotation2066506/range2066506/1
type: Range
persistent identity: range2066506
display id: range2066506
version: 1
top level: BBa_J18938
direction: direction/reverse
start: 1032
end: 1049
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066508
display id: annotation2066508
version: 1
top level: BBa_J18938
location: https://synbiohub.org/public/igem/BBa_J18938/annotation2066508/range2066508/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18938/annotation2066508/range2066508/1
type: Range
persistent identity: range2066508
display id: range2066508
version: 1
top level: BBa_J18938
direction: direction/reverse
start: 1050
end: 1055
orientation: inline

BBa_J18913
type: SequenceAnnotation
persistent identity: annotation2066510
display id: annotation2066510
version: 1
top level: BBa_J18938
location: https://synbiohub.org/public/igem/BBa_J18938/annotation2066510/range2066510/1
component: BBa_J18913

https://synbiohub.org/public/igem/BBa_J18938/annotation2066510/range2066510/1
type: Range
persistent identity: range2066510
display id: range2066510
version: 1
top level: BBa_J18938
direction: direction/reverse
start: 1056
end: 1190
orientation: inline

BBa_I757011
type: ComponentDefinition
persistent identity: BBa I757011
display id: BBa_I757011
version: 1
was generated by: iGEM to SBOL conversion
description: bla_frag1(aa 26-199) Fusion Part
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:07Z
mutable provenance: synthetic DNA by GeneArt
owned by: james
owned by: myers
mutable description: * first fragment of the split enzyme beta-lactamase * NgoMIV / AgeI protein fusion part * iGEM Team Freiburg 2007
discontinued: false
dominant: false
experience: experience/None
group u list: _125_
m user id: 0
owner id: 2006
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: between BioBrick 1.0 sites part is flanked 5' by NgoMIV and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions
star: false
creator: Freiburg iGEM 2007 team
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NgoMIV
sequence annotation: bla_frag1
sequence annotation: AgeI
sequence: https://synbiohub.org/public/igem/BBa_I757011_sequence/1

NgoMIV
type: SequenceAnnotation
persistent identity: annotation1954441
display id: annotation1954441
version: 1
top level: BBa_I757011
location: https://synbiohub.org/public/igem/BBa_I757011/annotation1954441/range1954441/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_I757011/annotation1954441/range1954441/1
type: Range
persistent identity: range1954441
display id: range1954441
version: 1
top level: BBa_I757011
direction: direction/forward
start: 4
end: 9
orientation: inline

bla_frag1
type: SequenceAnnotation
persistent identity: annotation1954942
display id: annotation1954942
version: 1
top level: BBa_I757011
location: https://synbiohub.org/public/igem/BBa_I757011/annotation1954942/range1954942/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_I757011/annotation1954942/range1954942/1
type: Range
persistent identity: range1954942
display id: range1954942
version: 1
top level: BBa_I757011
direction: direction/forward
start: 10
end: 534
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1954442
display id: annotation1954442
version: 1
top level: BBa_I757011
location: https://synbiohub.org/public/igem/BBa_I757011/annotation1954442/range1954442/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_I757011/annotation1954442/range1954442/1
type: Range
persistent identity: range1954442
display id: range1954442
version: 1
top level: BBa_I757011
direction: direction/forward
start: 535
end: 540
orientation: inline

Scar 25
type: ComponentDefinition
persistent identity: BBa B0105
display id: BBa_B0105
version: 1
was generated by: iGEM to SBOL conversion
description: RFC 25 Scar Sequence
created: 2009-10-14T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: BBF RFC 25
owned by: james
owned by: myers
mutable description: This is the scar produced by assembly using RFC 25. If you are assembling a composite part using RFC 25, you can insert this part and specify blunt assembly to get the desired sequence.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 25
owning group id: 397
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Simple DNA sequence
star: false
creator: Randy Rettberg
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: Scar 25
sequence: https://synbiohub.org/public/igem/BBa_B0105_sequence/1

Scar 25
type: SequenceAnnotation
persistent identity: annotation2041621
display id: annotation2041621
version: 1
top level: Scar 25
location: https://synbiohub.org/public/igem/BBa_B0105/annotation2041621/range2041621/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0105/annotation2041621/range2041621/1
type: Range
persistent identity: range2041621
display id: range2041621
version: 1
top level: Scar 25
direction: direction/forward
start: 1
end: 6
orientation: inline

T7start
type: ComponentDefinition
persistent identity: BBa J18912
display id: BBa_J18912
version: 1
was generated by: iGEM to SBOL conversion
description: T7 promoter + RBS + START
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: pET3a expression vector
owned by: james
owned by: myers
mutable description: Strong T7 promoter and RBS including start codon for protein expression in E. coli or in-vitro. Part is provided in RFC 25 assembly format.
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: * sequence is taken from the commonly used pET3a expression vector * deleted XbaI site between prom. and RBS * T7 promoter sequence (1-19) equals the one in BBa_I712074 * Conservative choice of part boundaries and modifications -- the part's size could probably be reduced.
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_J18912_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2065586
display id: annotation2065586
version: 1
top level: T7start
location: https://synbiohub.org/public/igem/BBa_J18912/annotation2065586/range2065586/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_J18912/annotation2065586/range2065586/1
type: Range
persistent identity: range2065586
display id: range2065586
version: 1
top level: T7start
direction: direction/forward
start: 81
end: 83
orientation: inline

preSCsite
type: ComponentDefinition
persistent identity: BBa J18919
display id: BBa_J18919
version: 1
was generated by: iGEM to SBOL conversion
description: preScission protease cleavage site (E. coli-optimized)
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: gene synthesis
owned by: james
owned by: myers
mutable description: cleavage site for PreScission??? protease. preScission protease cleavage site (PreScission??? protease is a genetically engineered form of human rhinovirus 3C protease with a GST fusion, allowing for facile cleavage and purification of GST-tagged proteins along with protease removal after recombinant protein cleavage. Enables low-temperature cleavage of fusion proteins containing the eight-residue recognition sequence.) cleavage site: * Leu-Glu-Val-Leu-Phe-Gln↓Gly-Pro References ============== Walker et al. (1994): Efficient and rapid affinity purification of proteins using recombinant fusion proteases. PMID: 7764949
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: * gene synthesis * codon optimized for E. coli
star: false
creator: Raik Gruenberg
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_J18919_sequence/1

5xGS
type: ComponentDefinition
persistent identity: BBa J18922
display id: BBa_J18922
version: 1
was generated by: iGEM to SBOL conversion
description: 10aa [GS]x linker
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: gene synthesis
owned by: james
owned by: myers
mutable description: Highly flexible 10 amino acid linker. Translates to [GS]_5. Codon-optimize for E. coli, yeast, mammalian.
discontinued: false
dominant: true
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: * gene synthesis * codon optimized for E. coli * experimental distance distributions are described in Neuweiler et al. 2006 papers.
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J18922_sequence/1

FKBP12
type: ComponentDefinition
persistent identity: BBa J18925
display id: BBa_J18925
version: 1
was generated by: iGEM to SBOL conversion
description: FKBP12
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: literature
owned by: james
owned by: myers
mutable description: The immunophilin protein FKBP12 (FK506-binding protein) is the target of the natural compounds FK506 and rapamycin. The FKBP/rapamycin or FKBP/FK506 complex then binds to several other proteins and suppresses immune reactions. Drug screening and design has turned up a selection of other small molecules that bind with high specificity to FKBP12 itself or to mutants of FKBP12. A second human protein, FRB is among the most prominent binding targets of the FKBP/rapamycin complex (using the "other face" of the rapamycin molecule). Rapamycin can herefore induce the hetero-dimerization of FKBP12 and FRB. == See also == * structure of FRB / FKBP / Rapamycin complex: PDB 1nsg (CRG-internal ID: rg0073)
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: * gene synthesis * codon optimized for E. coli
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J18925_sequence/1

HisTag
type: ComponentDefinition
persistent identity: BBa J18909
display id: BBa_J18909
version: 1
was generated by: iGEM to SBOL conversion
description: His tag (6xHis; E. coli - optimized)
created: 2010-01-15T12:00:00Z
modified: 2015-08-31T04:08:35Z
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
mutable description: Hexahistidine affinity tag. Binds zinc, nickel, or cobalt affinity resins. =Notes= This part is identical to BBa_I757013 (which does not seem to be available though). =References= Wikipedia article: [http://en.wikipedia.org/wiki/Polyhistidine-tag]
discontinued: false
dominant: false
experience: experience/None
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Simple repetition of the His codon most frequent in E. coli -- a more balanced codon-choice may be better.
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: PolyHis
sequence: https://synbiohub.org/public/igem/BBa_J18909_sequence/1

PolyHis
type: SequenceAnnotation
persistent identity: annotation2065463
display id: annotation2065463
version: 1
top level: HisTag
location: https://synbiohub.org/public/igem/BBa_J18909/annotation2065463/range2065463/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_J18909/annotation2065463/range2065463/1
type: Range
persistent identity: range2065463
display id: range2065463
version: 1
top level: HisTag
direction: direction/forward
start: 1
end: 18
orientation: inline

T7stop
type: ComponentDefinition
persistent identity: BBa J18913
display id: BBa_J18913
version: 1
was generated by: iGEM to SBOL conversion
description: T7 terminator (incl. STOP)
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: pET3a expression plasmid
owned by: james
owned by: myers
mutable description: T7 terminator for protein expression in E. coli or in vitro. Part is provided in R.F.C. 25 assembly format.
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: * taken from pET3a expression plasmid * added two STOP to 5' * conservative choice of part boundary, could probably be shortened
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_J18913_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2065587
display id: annotation2065587
version: 1
top level: T7stop
location: https://synbiohub.org/public/igem/BBa_J18913/annotation2065587/range2065587/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_J18913/annotation2065587/range2065587/1
type: Range
persistent identity: range2065587
display id: range2065587
version: 1
top level: T7stop
direction: direction/forward
start: 1
end: 6
orientation: inline

https://synbiohub.org/public/igem/BBa_J18938_sequence/1
type: Sequence
persistent identity: BBa J18938 sequence
display id: BBa_J18938_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagaccacaacggtttccctctagcaataattttgtttaactttaagaaggagatatacatatgaccggcggtgtgcaggtggaaaccattagcccgggtgatggccgtacctttccgaaacgtggccagacctgcgtggtgcattataccggcatgctggaagatggcaaaaaatttgatagcagccgcgatcgtaacaaaccgttcaaattcatgctgggcaaacaggaagtgattcgcggctgggaggaaggcgtggcgcagatgagcgttggccagcgtgcgaaactgaccatcagcccggattatgcgtatggcgcgaccggccatccgggtattattccgccgcatgcgaccctggtgtttgatgtggaactgctgaaactggaaaccggcggtagcggcagcggtagcggtagcggcagcaccggcatggccggccacccggaaaccctggccaaagtgaaagatgcggaagatcagctgggtgcgcgtgtgggctatattgaactggatctgaacagcggcaaaattctggaatcttttcgtccggaagaacgttttccgatgatgagcacctttaaagtgctgctgtgcggtgcggttctgagccgtattgatgcgggccaggaacagctgggccgtcgtattcattatagccagaacgatctggtggaatatagcccggtgaccgaaaaacatctgaccgatggcatgaccgtgggcgaactgtgcagcgcggcgattaccatgagcgataacaccgcggcgaacctgctgctgaccaccattggcggtccgaaagaactgaccgcgtttctgcgtaacatgggcgatcatgtgacccgtctggatcgttgggaaccggaactgaacgaagcgattccgaacgatgaacgtgataccaccaccccggtggccatggcgaccaccctgcgtaaactgctgaccggcgaactgctgggcaccggttaaaccggcctggaagtgctgtttcagggcccgaccggccatcatcatcatcatcataccggctagtgactgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I757011_sequence/1
type: Sequence
persistent identity: BBa I757011 sequence
display id: BBa_I757011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccggccacccggaaaccctggccaaagtgaaagatgcggaagatcagctgggtgcgcgtgtgggctatattgaactggatctgaacagcggcaaaattctggaatcttttcgtccggaagaacgttttccgatgatgagcacctttaaagtgctgctgtgcggtgcggttctgagccgtattgatgcgggccaggaacagctgggccgtcgtattcattatagccagaacgatctggtggaatatagcccggtgaccgaaaaacatctgaccgatggcatgaccgtgggcgaactgtgcagcgcggcgattaccatgagcgataacaccgcggcgaacctgctgctgaccaccattggcggtccgaaagaactgaccgcgtttctgcgtaacatgggcgatcatgtgacccgtctggatcgttgggaaccggaactgaacgaagcgattccgaacgatgaacgtgataccaccaccccggtggccatggcgaccaccctgcgtaaactgctgaccggcgaactgctgggcaccggttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0105_sequence/1
type: Sequence
persistent identity: BBa B0105 sequence
display id: BBa_B0105_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: accggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18912_sequence/1
type: Sequence
persistent identity: BBa J18912 sequence
display id: BBa_J18912_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagaccacaacggtttccctctagcaataattttgtttaactttaagaaggagatatacatatg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18919_sequence/1
type: Sequence
persistent identity: BBa J18919 sequence
display id: BBa_J18919_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctggaagtgctgtttcagggcccg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18909_sequence/1
type: Sequence
persistent identity: BBa J18909 sequence
display id: BBa_J18909_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: catcatcatcatcatcat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18913_sequence/1
type: Sequence
persistent identity: BBa J18913 sequence
display id: BBa_J18913_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tagtgactgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18922_sequence/1
type: Sequence
persistent identity: BBa J18922 sequence
display id: BBa_J18922_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtagcggcagcggtagcggtagcggcagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18925_sequence/1
type: Sequence
persistent identity: BBa J18925 sequence
display id: BBa_J18925_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtgtgcaggtggaaaccattagcccgggtgatggccgtacctttccgaaacgtggccagacctgcgtggtgcattataccggcatgctggaagatggcaaaaaatttgatagcagccgcgatcgtaacaaaccgttcaaattcatgctgggcaaacaggaagtgattcgcggctgggaggaaggcgtggcgcagatgagcgttggccagcgtgcgaaactgaccatcagcccggattatgcgtatggcgcgaccggccatccgggtattattccgccgcatgcgaccctggtgtttgatgtggaactgctgaaactggaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z