BBa_J18940
type: ComponentDefinition
persistent identity: BBa J18940
display id: BBa_J18940
version: 1
was generated by: iGEM to SBOL conversion
description: FRB ~ GLucFrag1
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: synthetic
owned by: james
owned by: myers
mutable description: P08 expression cassette from Gruenberg et al. 2010 series of interaction reporter proteins. All proteins share the same architecture: * T7Start-domain1-5xGS-domain2-preSCsite-HisTag-T7Stop Only Domains1 and 2 vary and are taken from a list of protein interaction input and output (readout) parts. ===Expression=== Proteins were expressed in E. coli BL21 using T7 Polymerase and were purified by His-tag affinity. ===Reference=== * Gruenberg, Ferrar, van der Sloot, Serrano (2010): Building Blocks for Protein Interaction Devices
discontinued: false
dominant: false
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructs were assembled according to R.F.C. 25. All parts were codon optimized for E. coli.
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
component: BBa_J18912
component: BBa_J18926
component: BBa_J18928
component: BBa_B0105
component: BBa_J18922
component: BBa_J18919
component: BBa_J18909
component: BBa_J18913
sequence annotation: BBa_J18912
sequence annotation: BBa_B0105
sequence annotation: BBa_J18926
sequence annotation: BBa_J18922
sequence annotation: BBa_J18928
sequence annotation: BBa_J18919
sequence annotation: BBa_J18909
sequence annotation: BBa_J18913
sequence: https://synbiohub.org/public/igem/BBa_J18940_sequence/1

BBa_J18912
type: Component
persistent identity: component2066537
display id: component2066537
version: 1
top level: BBa_J18940
definition: T7start
access: public

BBa_J18926
type: Component
persistent identity: component2066540
display id: component2066540
version: 1
top level: BBa_J18940
definition: FRB
access: public

BBa_J18928
type: Component
persistent identity: component2066546
display id: component2066546
version: 1
top level: BBa_J18940
definition: GLucFrag1
access: public

BBa_B0105
type: Component
persistent identity: component2066539
display id: component2066539
version: 1
top level: BBa_J18940
definition: Scar 25
access: public

BBa_J18922
type: Component
persistent identity: component2066543
display id: component2066543
version: 1
top level: BBa_J18940
definition: 5xGS
access: public

BBa_B0105
type: Component
persistent identity: component2066545
display id: component2066545
version: 1
top level: BBa_J18940
definition: Scar 25
access: public

BBa_B0105
type: Component
persistent identity: component2066542
display id: component2066542
version: 1
top level: BBa_J18940
definition: Scar 25
access: public

BBa_J18919
type: Component
persistent identity: component2066549
display id: component2066549
version: 1
top level: BBa_J18940
definition: preSCsite
access: public

BBa_B0105
type: Component
persistent identity: component2066548
display id: component2066548
version: 1
top level: BBa_J18940
definition: Scar 25
access: public

BBa_J18909
type: Component
persistent identity: component2066553
display id: component2066553
version: 1
top level: BBa_J18940
definition: HisTag
access: public

BBa_B0105
type: Component
persistent identity: component2066551
display id: component2066551
version: 1
top level: BBa_J18940
definition: Scar 25
access: public

BBa_J18913
type: Component
persistent identity: component2066557
display id: component2066557
version: 1
top level: BBa_J18940
definition: T7stop
access: public

BBa_B0105
type: Component
persistent identity: component2066555
display id: component2066555
version: 1
top level: BBa_J18940
definition: Scar 25
access: public

BBa_J18912
type: SequenceAnnotation
persistent identity: annotation2066537
display id: annotation2066537
version: 1
top level: BBa_J18940
location: https://synbiohub.org/public/igem/BBa_J18940/annotation2066537/range2066537/1
component: BBa_J18912

https://synbiohub.org/public/igem/BBa_J18940/annotation2066537/range2066537/1
type: Range
persistent identity: range2066537
display id: range2066537
version: 1
top level: BBa_J18940
direction: direction/reverse
start: 1
end: 83
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066539
display id: annotation2066539
version: 1
top level: BBa_J18940
location: https://synbiohub.org/public/igem/BBa_J18940/annotation2066539/range2066539/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18940/annotation2066539/range2066539/1
type: Range
persistent identity: range2066539
display id: range2066539
version: 1
top level: BBa_J18940
direction: direction/reverse
start: 84
end: 89
orientation: inline

BBa_J18926
type: SequenceAnnotation
persistent identity: annotation2066540
display id: annotation2066540
version: 1
top level: BBa_J18940
location: https://synbiohub.org/public/igem/BBa_J18940/annotation2066540/range2066540/1
component: BBa_J18926

https://synbiohub.org/public/igem/BBa_J18940/annotation2066540/range2066540/1
type: Range
persistent identity: range2066540
display id: range2066540
version: 1
top level: BBa_J18940
direction: direction/reverse
start: 90
end: 368
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066542
display id: annotation2066542
version: 1
top level: BBa_J18940
location: https://synbiohub.org/public/igem/BBa_J18940/annotation2066542/range2066542/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18940/annotation2066542/range2066542/1
type: Range
persistent identity: range2066542
display id: range2066542
version: 1
top level: BBa_J18940
direction: direction/reverse
start: 369
end: 374
orientation: inline

BBa_J18922
type: SequenceAnnotation
persistent identity: annotation2066543
display id: annotation2066543
version: 1
top level: BBa_J18940
location: https://synbiohub.org/public/igem/BBa_J18940/annotation2066543/range2066543/1
component: BBa_J18922

https://synbiohub.org/public/igem/BBa_J18940/annotation2066543/range2066543/1
type: Range
persistent identity: range2066543
display id: range2066543
version: 1
top level: BBa_J18940
direction: direction/reverse
start: 375
end: 404
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066545
display id: annotation2066545
version: 1
top level: BBa_J18940
location: https://synbiohub.org/public/igem/BBa_J18940/annotation2066545/range2066545/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18940/annotation2066545/range2066545/1
type: Range
persistent identity: range2066545
display id: range2066545
version: 1
top level: BBa_J18940
direction: direction/reverse
start: 405
end: 410
orientation: inline

BBa_J18928
type: SequenceAnnotation
persistent identity: annotation2066546
display id: annotation2066546
version: 1
top level: BBa_J18940
location: https://synbiohub.org/public/igem/BBa_J18940/annotation2066546/range2066546/1
component: BBa_J18928

https://synbiohub.org/public/igem/BBa_J18940/annotation2066546/range2066546/1
type: Range
persistent identity: range2066546
display id: range2066546
version: 1
top level: BBa_J18940
direction: direction/reverse
start: 411
end: 686
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066548
display id: annotation2066548
version: 1
top level: BBa_J18940
location: https://synbiohub.org/public/igem/BBa_J18940/annotation2066548/range2066548/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18940/annotation2066548/range2066548/1
type: Range
persistent identity: range2066548
display id: range2066548
version: 1
top level: BBa_J18940
direction: direction/reverse
start: 687
end: 692
orientation: inline

BBa_J18919
type: SequenceAnnotation
persistent identity: annotation2066549
display id: annotation2066549
version: 1
top level: BBa_J18940
location: https://synbiohub.org/public/igem/BBa_J18940/annotation2066549/range2066549/1
component: BBa_J18919

https://synbiohub.org/public/igem/BBa_J18940/annotation2066549/range2066549/1
type: Range
persistent identity: range2066549
display id: range2066549
version: 1
top level: BBa_J18940
direction: direction/reverse
start: 693
end: 716
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066551
display id: annotation2066551
version: 1
top level: BBa_J18940
location: https://synbiohub.org/public/igem/BBa_J18940/annotation2066551/range2066551/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18940/annotation2066551/range2066551/1
type: Range
persistent identity: range2066551
display id: range2066551
version: 1
top level: BBa_J18940
direction: direction/reverse
start: 717
end: 722
orientation: inline

BBa_J18909
type: SequenceAnnotation
persistent identity: annotation2066553
display id: annotation2066553
version: 1
top level: BBa_J18940
location: https://synbiohub.org/public/igem/BBa_J18940/annotation2066553/range2066553/1
component: BBa_J18909

https://synbiohub.org/public/igem/BBa_J18940/annotation2066553/range2066553/1
type: Range
persistent identity: range2066553
display id: range2066553
version: 1
top level: BBa_J18940
direction: direction/reverse
start: 723
end: 740
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066555
display id: annotation2066555
version: 1
top level: BBa_J18940
location: https://synbiohub.org/public/igem/BBa_J18940/annotation2066555/range2066555/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18940/annotation2066555/range2066555/1
type: Range
persistent identity: range2066555
display id: range2066555
version: 1
top level: BBa_J18940
direction: direction/reverse
start: 741
end: 746
orientation: inline

BBa_J18913
type: SequenceAnnotation
persistent identity: annotation2066557
display id: annotation2066557
version: 1
top level: BBa_J18940
location: https://synbiohub.org/public/igem/BBa_J18940/annotation2066557/range2066557/1
component: BBa_J18913

https://synbiohub.org/public/igem/BBa_J18940/annotation2066557/range2066557/1
type: Range
persistent identity: range2066557
display id: range2066557
version: 1
top level: BBa_J18940
direction: direction/reverse
start: 747
end: 881
orientation: inline

Scar 25
type: ComponentDefinition
persistent identity: BBa B0105
display id: BBa_B0105
version: 1
was generated by: iGEM to SBOL conversion
description: RFC 25 Scar Sequence
created: 2009-10-14T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: BBF RFC 25
owned by: james
owned by: myers
mutable description: This is the scar produced by assembly using RFC 25. If you are assembling a composite part using RFC 25, you can insert this part and specify blunt assembly to get the desired sequence.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 25
owning group id: 397
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Simple DNA sequence
star: false
creator: Randy Rettberg
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: Scar 25
sequence: https://synbiohub.org/public/igem/BBa_B0105_sequence/1

Scar 25
type: SequenceAnnotation
persistent identity: annotation2041621
display id: annotation2041621
version: 1
top level: Scar 25
location: https://synbiohub.org/public/igem/BBa_B0105/annotation2041621/range2041621/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0105/annotation2041621/range2041621/1
type: Range
persistent identity: range2041621
display id: range2041621
version: 1
top level: Scar 25
direction: direction/forward
start: 1
end: 6
orientation: inline

T7start
type: ComponentDefinition
persistent identity: BBa J18912
display id: BBa_J18912
version: 1
was generated by: iGEM to SBOL conversion
description: T7 promoter + RBS + START
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: pET3a expression vector
owned by: james
owned by: myers
mutable description: Strong T7 promoter and RBS including start codon for protein expression in E. coli or in-vitro. Part is provided in RFC 25 assembly format.
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: * sequence is taken from the commonly used pET3a expression vector * deleted XbaI site between prom. and RBS * T7 promoter sequence (1-19) equals the one in BBa_I712074 * Conservative choice of part boundaries and modifications -- the part's size could probably be reduced.
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_J18912_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2065586
display id: annotation2065586
version: 1
top level: T7start
location: https://synbiohub.org/public/igem/BBa_J18912/annotation2065586/range2065586/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_J18912/annotation2065586/range2065586/1
type: Range
persistent identity: range2065586
display id: range2065586
version: 1
top level: T7start
direction: direction/forward
start: 81
end: 83
orientation: inline

preSCsite
type: ComponentDefinition
persistent identity: BBa J18919
display id: BBa_J18919
version: 1
was generated by: iGEM to SBOL conversion
description: preScission protease cleavage site (E. coli-optimized)
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: gene synthesis
owned by: james
owned by: myers
mutable description: cleavage site for PreScission??? protease. preScission protease cleavage site (PreScission??? protease is a genetically engineered form of human rhinovirus 3C protease with a GST fusion, allowing for facile cleavage and purification of GST-tagged proteins along with protease removal after recombinant protein cleavage. Enables low-temperature cleavage of fusion proteins containing the eight-residue recognition sequence.) cleavage site: * Leu-Glu-Val-Leu-Phe-Gln↓Gly-Pro References ============== Walker et al. (1994): Efficient and rapid affinity purification of proteins using recombinant fusion proteases. PMID: 7764949
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: * gene synthesis * codon optimized for E. coli
star: false
creator: Raik Gruenberg
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_J18919_sequence/1

5xGS
type: ComponentDefinition
persistent identity: BBa J18922
display id: BBa_J18922
version: 1
was generated by: iGEM to SBOL conversion
description: 10aa [GS]x linker
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: gene synthesis
owned by: james
owned by: myers
mutable description: Highly flexible 10 amino acid linker. Translates to [GS]_5. Codon-optimize for E. coli, yeast, mammalian.
discontinued: false
dominant: true
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: * gene synthesis * codon optimized for E. coli * experimental distance distributions are described in Neuweiler et al. 2006 papers.
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J18922_sequence/1

FRB
type: ComponentDefinition
persistent identity: BBa J18926
display id: BBa_J18926
version: 1
was generated by: iGEM to SBOL conversion
description: FRB(T2098L)
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: human mTOR (aa sequence) gene synthesis
owned by: james
owned by: myers
mutable description: Modified FKBP12-rapamycin-binding domain (FRB) of human mTOR Kinase (=mammalian Target of Rapamycin, also called FRAP = FKBP12-rapamycin associated protein). In presence of the immunosupressant drug rapamycin, FRB forms a high-affinity complex with FKBP12. There is no detectable FKBP12 - FRB interaction in absence of rapamycin. ===Modification for a non-toxic dimerizer=== Rapamycin arrests growth and proliferation of many cell types (reviewed in Jacinto and Hall, 2003) by inhibiting protein translation. The mutation T2089L (mTor numbering) makes FRB binding to a non-toxic Rapamycin analogue (rapalogue) AP21967 as well as the original Rapamycin. The FRB - FKBP12 heterodimerization can then be triggered by both Rapamycin and the non-toxic rapalogue. This variant of FRB and the synthetic dimerizer AP21967 are the basis of the Argent(tm) heterodimerization kit from Ariad Pharmaceuticals Inc. '''Note:''' Two additional mutations would convert FRB into FRB* which is used for conditional (drug-reversible) degradation of fusion proteins. These two additional mutations are not implemented in this part. ===Protein Parameters:=== * Number of amino acids: 93 * Molecular weight: 11285.9 * Theoretical pI: 6.47
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: * aa translation from human mTOR, * mutation T2089L (verified against pC4-RHE from Ariad) * GeneArt codon optimization for E. coli * gene synthesis
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J18926_sequence/1

GLucFrag1
type: ComponentDefinition
persistent identity: BBa J18928
display id: BBa_J18928
version: 1
was generated by: iGEM to SBOL conversion
description: Gaussia princeps Luciferase fragment 1
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: gene synthesis
owned by: james
owned by: myers
mutable description: N-terminal fragment of H. gaussia luciferase for PCA. '''Disulfide bonds:''' * likely, many Cys in sequence ===Purification=== Inouye & Sahara (2008) discuss the purification and in-vitro activity of different Luciferases, including Gaussia. They indicate that Gaussia Luciferase was previously purified from insoluable fractions -- perhaps due to the high Cys content. Goerke et al (2008) Provide optimized in-vitro expression conditions for Gaussia Luciferase based on tuning the strain from which the cell-free lysate is produced. ===References=== http://www.ncbi.nlm.nih.gov/pubmed/17099704 Remy I, Michnick SW. (2006) A highly sensitive protein-protein interaction assay based on Gaussia luciferase. http://nar.oxfordjournals.org/cgi/content/full/27/13/e4#hd1 Maroun M, Aronheim A. (2007) A novel in vivo assay for the analysis of protein-protein interaction. PMID: 10373602 http://www.ncbi.nlm.nih.gov/pubmed/19373229 Tannous BA. (2009) Gaussia luciferase reporter assay for monitoring biological processes in culture and in vivo. http://www.ncbi.nlm.nih.gov/pubmed/18789309 Inouye S, Sahara Y. (2008) Soluble protein expression in E. coli cells using IgG-binding domain of protein A as a solubilizing partner in the cold induced system. http://www.ncbi.nlm.nih.gov/pubmed/18555198 Goerke AR, Loening AM, Gambhir SS, Swartz JR. (2008) Cell-free metabolic engineering promotes high-level production of bioactive Gaussia princeps luciferase.
discontinued: false
dominant: false
experience: experience/Issues
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: * gene synthesis * codon optimized for E. coli
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J18928_sequence/1

HisTag
type: ComponentDefinition
persistent identity: BBa J18909
display id: BBa_J18909
version: 1
was generated by: iGEM to SBOL conversion
description: His tag (6xHis; E. coli - optimized)
created: 2010-01-15T12:00:00Z
modified: 2015-08-31T04:08:35Z
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
mutable description: Hexahistidine affinity tag. Binds zinc, nickel, or cobalt affinity resins. =Notes= This part is identical to BBa_I757013 (which does not seem to be available though). =References= Wikipedia article: [http://en.wikipedia.org/wiki/Polyhistidine-tag]
discontinued: false
dominant: false
experience: experience/None
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Simple repetition of the His codon most frequent in E. coli -- a more balanced codon-choice may be better.
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: PolyHis
sequence: https://synbiohub.org/public/igem/BBa_J18909_sequence/1

PolyHis
type: SequenceAnnotation
persistent identity: annotation2065463
display id: annotation2065463
version: 1
top level: HisTag
location: https://synbiohub.org/public/igem/BBa_J18909/annotation2065463/range2065463/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_J18909/annotation2065463/range2065463/1
type: Range
persistent identity: range2065463
display id: range2065463
version: 1
top level: HisTag
direction: direction/forward
start: 1
end: 18
orientation: inline

T7stop
type: ComponentDefinition
persistent identity: BBa J18913
display id: BBa_J18913
version: 1
was generated by: iGEM to SBOL conversion
description: T7 terminator (incl. STOP)
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: pET3a expression plasmid
owned by: james
owned by: myers
mutable description: T7 terminator for protein expression in E. coli or in vitro. Part is provided in R.F.C. 25 assembly format.
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: * taken from pET3a expression plasmid * added two STOP to 5' * conservative choice of part boundary, could probably be shortened
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_J18913_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2065587
display id: annotation2065587
version: 1
top level: T7stop
location: https://synbiohub.org/public/igem/BBa_J18913/annotation2065587/range2065587/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_J18913/annotation2065587/range2065587/1
type: Range
persistent identity: range2065587
display id: range2065587
version: 1
top level: T7stop
direction: direction/forward
start: 1
end: 6
orientation: inline

https://synbiohub.org/public/igem/BBa_J18940_sequence/1
type: Sequence
persistent identity: BBa J18940 sequence
display id: BBa_J18940_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagaccacaacggtttccctctagcaataattttgtttaactttaagaaggagatatacatatgaccggcatcctgtggcatgaaatgtggcatgaaggcctggaagaagcgagccgtctgtattttggcgaacgtaacgtgaaaggcatgtttgaagtgctggaaccgctgcatgccatgatggaacgtggcccgcagaccctgaaagaaaccagctttaaccaggcgtatggccgtgatctgatggaagcgcaggaatggtgccgtaaatacatgaaaagcggcaacgtgaaagatctgctgcaagcgtgggatctgtattatcatgtgtttcgccgcatcagcaaaaccggcggtagcggcagcggtagcggtagcggcagcaccggcaaaccgaccgaaaacaacgaagattttaacattgtggcggtggcgagcaactttgcgaccaccgatctggatgcggatcgtggcaaactgccgggcaaaaaactgccgctggaagtgctgaaagaaatggaagcgaacgcgcgtaaagccggttgcacccgtggctgcctgatttgcctgagccatattaaatgcaccccgaaaatgaaaaaatttatcccgggtcgttgccatacctatgaaggcgataaagaaagcgcgcagggcggcattggcaccggcctggaagtgctgtttcagggcccgaccggccatcatcatcatcatcataccggctagtgactgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0105_sequence/1
type: Sequence
persistent identity: BBa B0105 sequence
display id: BBa_B0105_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: accggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18912_sequence/1
type: Sequence
persistent identity: BBa J18912 sequence
display id: BBa_J18912_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagaccacaacggtttccctctagcaataattttgtttaactttaagaaggagatatacatatg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18919_sequence/1
type: Sequence
persistent identity: BBa J18919 sequence
display id: BBa_J18919_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctggaagtgctgtttcagggcccg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18922_sequence/1
type: Sequence
persistent identity: BBa J18922 sequence
display id: BBa_J18922_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtagcggcagcggtagcggtagcggcagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18926_sequence/1
type: Sequence
persistent identity: BBa J18926 sequence
display id: BBa_J18926_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atcctgtggcatgaaatgtggcatgaaggcctggaagaagcgagccgtctgtattttggcgaacgtaacgtgaaaggcatgtttgaagtgctggaaccgctgcatgccatgatggaacgtggcccgcagaccctgaaagaaaccagctttaaccaggcgtatggccgtgatctgatggaagcgcaggaatggtgccgtaaatacatgaaaagcggcaacgtgaaagatctgctgcaagcgtgggatctgtattatcatgtgtttcgccgcatcagcaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18928_sequence/1
type: Sequence
persistent identity: BBa J18928 sequence
display id: BBa_J18928_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaccgaccgaaaacaacgaagattttaacattgtggcggtggcgagcaactttgcgaccaccgatctggatgcggatcgtggcaaactgccgggcaaaaaactgccgctggaagtgctgaaagaaatggaagcgaacgcgcgtaaagccggttgcacccgtggctgcctgatttgcctgagccatattaaatgcaccccgaaaatgaaaaaatttatcccgggtcgttgccatacctatgaaggcgataaagaaagcgcgcagggcggcattggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18909_sequence/1
type: Sequence
persistent identity: BBa J18909 sequence
display id: BBa_J18909_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: catcatcatcatcatcat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18913_sequence/1
type: Sequence
persistent identity: BBa J18913 sequence
display id: BBa_J18913_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tagtgactgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggat
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z