BBa_J18950
type: ComponentDefinition
persistent identity: BBa J18950
display id: BBa_J18950
version: 1
was generated by: iGEM to SBOL conversion
description: mCitrine ~ Lov2
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: synthetic
owned by: james
owned by: myers
mutable description: P18 expression cassette from Gruenberg et al. 2010 series of interaction reporter proteins. All proteins share the same architecture: * T7Start-domain1-5xGS-domain2-preSCsite-HisTag-T7Stop Only Domains1 and 2 vary and are taken from a list of protein interaction input and output (readout) parts. ===Expression=== Proteins were expressed in E. coli BL21 using T7 Polymerase and were purified by His-tag affinity. ===Reference=== * Gruenberg, Ferrar, van der Sloot, Serrano (2010): Building Blocks for Protein Interaction Devices
discontinued: false
dominant: false
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructs were assembled according to R.F.C. 25. All parts were codon optimized for E. coli.
star: false
creator: Raik Gruenberg
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_J18912
component: BBa_J18931
component: BBa_B0105
component: BBa_J18913
component: BBa_J18922
component: BBa_J18927
component: BBa_J18919
component: BBa_J18909
sequence annotation: BBa_J18912
sequence annotation: BBa_B0105
sequence annotation: BBa_J18931
sequence annotation: BBa_J18922
sequence annotation: BBa_J18927
sequence annotation: BBa_J18919
sequence annotation: BBa_J18909
sequence annotation: BBa_J18913
sequence: https://synbiohub.org/public/igem/BBa_J18950_sequence/1

BBa_J18912
type: Component
persistent identity: component2066760
display id: component2066760
version: 1
top level: BBa_J18950
definition: T7start
access: public

BBa_J18931
type: Component
persistent identity: component2066763
display id: component2066763
version: 1
top level: BBa_J18950
definition: mCitrine
access: public

BBa_B0105
type: Component
persistent identity: component2066762
display id: component2066762
version: 1
top level: BBa_J18950
definition: Scar 25
access: public

BBa_J18913
type: Component
persistent identity: component2066780
display id: component2066780
version: 1
top level: BBa_J18950
definition: T7stop
access: public

BBa_B0105
type: Component
persistent identity: component2066778
display id: component2066778
version: 1
top level: BBa_J18950
definition: Scar 25
access: public

BBa_J18922
type: Component
persistent identity: component2066766
display id: component2066766
version: 1
top level: BBa_J18950
definition: 5xGS
access: public

BBa_B0105
type: Component
persistent identity: component2066765
display id: component2066765
version: 1
top level: BBa_J18950
definition: Scar 25
access: public

BBa_J18927
type: Component
persistent identity: component2066769
display id: component2066769
version: 1
top level: BBa_J18950
definition: Lov2
access: public

BBa_B0105
type: Component
persistent identity: component2066768
display id: component2066768
version: 1
top level: BBa_J18950
definition: Scar 25
access: public

BBa_J18919
type: Component
persistent identity: component2066772
display id: component2066772
version: 1
top level: BBa_J18950
definition: preSCsite
access: public

BBa_B0105
type: Component
persistent identity: component2066771
display id: component2066771
version: 1
top level: BBa_J18950
definition: Scar 25
access: public

BBa_J18909
type: Component
persistent identity: component2066776
display id: component2066776
version: 1
top level: BBa_J18950
definition: HisTag
access: public

BBa_B0105
type: Component
persistent identity: component2066774
display id: component2066774
version: 1
top level: BBa_J18950
definition: Scar 25
access: public

BBa_J18912
type: SequenceAnnotation
persistent identity: annotation2066760
display id: annotation2066760
version: 1
top level: BBa_J18950
location: https://synbiohub.org/public/igem/BBa_J18950/annotation2066760/range2066760/1
component: BBa_J18912

https://synbiohub.org/public/igem/BBa_J18950/annotation2066760/range2066760/1
type: Range
persistent identity: range2066760
display id: range2066760
version: 1
top level: BBa_J18950
direction: direction/reverse
start: 1
end: 83
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066762
display id: annotation2066762
version: 1
top level: BBa_J18950
location: https://synbiohub.org/public/igem/BBa_J18950/annotation2066762/range2066762/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18950/annotation2066762/range2066762/1
type: Range
persistent identity: range2066762
display id: range2066762
version: 1
top level: BBa_J18950
direction: direction/reverse
start: 84
end: 89
orientation: inline

BBa_J18931
type: SequenceAnnotation
persistent identity: annotation2066763
display id: annotation2066763
version: 1
top level: BBa_J18950
location: https://synbiohub.org/public/igem/BBa_J18950/annotation2066763/range2066763/1
component: BBa_J18931

https://synbiohub.org/public/igem/BBa_J18950/annotation2066763/range2066763/1
type: Range
persistent identity: range2066763
display id: range2066763
version: 1
top level: BBa_J18950
direction: direction/reverse
start: 90
end: 803
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066765
display id: annotation2066765
version: 1
top level: BBa_J18950
location: https://synbiohub.org/public/igem/BBa_J18950/annotation2066765/range2066765/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18950/annotation2066765/range2066765/1
type: Range
persistent identity: range2066765
display id: range2066765
version: 1
top level: BBa_J18950
direction: direction/reverse
start: 804
end: 809
orientation: inline

BBa_J18922
type: SequenceAnnotation
persistent identity: annotation2066766
display id: annotation2066766
version: 1
top level: BBa_J18950
location: https://synbiohub.org/public/igem/BBa_J18950/annotation2066766/range2066766/1
component: BBa_J18922

https://synbiohub.org/public/igem/BBa_J18950/annotation2066766/range2066766/1
type: Range
persistent identity: range2066766
display id: range2066766
version: 1
top level: BBa_J18950
direction: direction/reverse
start: 810
end: 839
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066768
display id: annotation2066768
version: 1
top level: BBa_J18950
location: https://synbiohub.org/public/igem/BBa_J18950/annotation2066768/range2066768/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18950/annotation2066768/range2066768/1
type: Range
persistent identity: range2066768
display id: range2066768
version: 1
top level: BBa_J18950
direction: direction/reverse
start: 840
end: 845
orientation: inline

BBa_J18927
type: SequenceAnnotation
persistent identity: annotation2066769
display id: annotation2066769
version: 1
top level: BBa_J18950
location: https://synbiohub.org/public/igem/BBa_J18950/annotation2066769/range2066769/1
component: BBa_J18927

https://synbiohub.org/public/igem/BBa_J18950/annotation2066769/range2066769/1
type: Range
persistent identity: range2066769
display id: range2066769
version: 1
top level: BBa_J18950
direction: direction/reverse
start: 846
end: 1259
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066771
display id: annotation2066771
version: 1
top level: BBa_J18950
location: https://synbiohub.org/public/igem/BBa_J18950/annotation2066771/range2066771/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18950/annotation2066771/range2066771/1
type: Range
persistent identity: range2066771
display id: range2066771
version: 1
top level: BBa_J18950
direction: direction/reverse
start: 1260
end: 1265
orientation: inline

BBa_J18919
type: SequenceAnnotation
persistent identity: annotation2066772
display id: annotation2066772
version: 1
top level: BBa_J18950
location: https://synbiohub.org/public/igem/BBa_J18950/annotation2066772/range2066772/1
component: BBa_J18919

https://synbiohub.org/public/igem/BBa_J18950/annotation2066772/range2066772/1
type: Range
persistent identity: range2066772
display id: range2066772
version: 1
top level: BBa_J18950
direction: direction/reverse
start: 1266
end: 1289
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066774
display id: annotation2066774
version: 1
top level: BBa_J18950
location: https://synbiohub.org/public/igem/BBa_J18950/annotation2066774/range2066774/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18950/annotation2066774/range2066774/1
type: Range
persistent identity: range2066774
display id: range2066774
version: 1
top level: BBa_J18950
direction: direction/reverse
start: 1290
end: 1295
orientation: inline

BBa_J18909
type: SequenceAnnotation
persistent identity: annotation2066776
display id: annotation2066776
version: 1
top level: BBa_J18950
location: https://synbiohub.org/public/igem/BBa_J18950/annotation2066776/range2066776/1
component: BBa_J18909

https://synbiohub.org/public/igem/BBa_J18950/annotation2066776/range2066776/1
type: Range
persistent identity: range2066776
display id: range2066776
version: 1
top level: BBa_J18950
direction: direction/reverse
start: 1296
end: 1313
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066778
display id: annotation2066778
version: 1
top level: BBa_J18950
location: https://synbiohub.org/public/igem/BBa_J18950/annotation2066778/range2066778/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18950/annotation2066778/range2066778/1
type: Range
persistent identity: range2066778
display id: range2066778
version: 1
top level: BBa_J18950
direction: direction/reverse
start: 1314
end: 1319
orientation: inline

BBa_J18913
type: SequenceAnnotation
persistent identity: annotation2066780
display id: annotation2066780
version: 1
top level: BBa_J18950
location: https://synbiohub.org/public/igem/BBa_J18950/annotation2066780/range2066780/1
component: BBa_J18913

https://synbiohub.org/public/igem/BBa_J18950/annotation2066780/range2066780/1
type: Range
persistent identity: range2066780
display id: range2066780
version: 1
top level: BBa_J18950
direction: direction/reverse
start: 1320
end: 1454
orientation: inline

Scar 25
type: ComponentDefinition
persistent identity: BBa B0105
display id: BBa_B0105
version: 1
was generated by: iGEM to SBOL conversion
description: RFC 25 Scar Sequence
created: 2009-10-14T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: BBF RFC 25
owned by: james
owned by: myers
mutable description: This is the scar produced by assembly using RFC 25. If you are assembling a composite part using RFC 25, you can insert this part and specify blunt assembly to get the desired sequence.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 25
owning group id: 397
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Simple DNA sequence
star: false
creator: Randy Rettberg
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: Scar 25
sequence: https://synbiohub.org/public/igem/BBa_B0105_sequence/1

Scar 25
type: SequenceAnnotation
persistent identity: annotation2041621
display id: annotation2041621
version: 1
top level: Scar 25
location: https://synbiohub.org/public/igem/BBa_B0105/annotation2041621/range2041621/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0105/annotation2041621/range2041621/1
type: Range
persistent identity: range2041621
display id: range2041621
version: 1
top level: Scar 25
direction: direction/forward
start: 1
end: 6
orientation: inline

T7start
type: ComponentDefinition
persistent identity: BBa J18912
display id: BBa_J18912
version: 1
was generated by: iGEM to SBOL conversion
description: T7 promoter + RBS + START
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: pET3a expression vector
owned by: james
owned by: myers
mutable description: Strong T7 promoter and RBS including start codon for protein expression in E. coli or in-vitro. Part is provided in RFC 25 assembly format.
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: * sequence is taken from the commonly used pET3a expression vector * deleted XbaI site between prom. and RBS * T7 promoter sequence (1-19) equals the one in BBa_I712074 * Conservative choice of part boundaries and modifications -- the part's size could probably be reduced.
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_J18912_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2065586
display id: annotation2065586
version: 1
top level: T7start
location: https://synbiohub.org/public/igem/BBa_J18912/annotation2065586/range2065586/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_J18912/annotation2065586/range2065586/1
type: Range
persistent identity: range2065586
display id: range2065586
version: 1
top level: T7start
direction: direction/forward
start: 81
end: 83
orientation: inline

preSCsite
type: ComponentDefinition
persistent identity: BBa J18919
display id: BBa_J18919
version: 1
was generated by: iGEM to SBOL conversion
description: preScission protease cleavage site (E. coli-optimized)
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: gene synthesis
owned by: james
owned by: myers
mutable description: cleavage site for PreScission??? protease. preScission protease cleavage site (PreScission??? protease is a genetically engineered form of human rhinovirus 3C protease with a GST fusion, allowing for facile cleavage and purification of GST-tagged proteins along with protease removal after recombinant protein cleavage. Enables low-temperature cleavage of fusion proteins containing the eight-residue recognition sequence.) cleavage site: * Leu-Glu-Val-Leu-Phe-Gln↓Gly-Pro References ============== Walker et al. (1994): Efficient and rapid affinity purification of proteins using recombinant fusion proteases. PMID: 7764949
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: * gene synthesis * codon optimized for E. coli
star: false
creator: Raik Gruenberg
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_J18919_sequence/1

5xGS
type: ComponentDefinition
persistent identity: BBa J18922
display id: BBa_J18922
version: 1
was generated by: iGEM to SBOL conversion
description: 10aa [GS]x linker
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: gene synthesis
owned by: james
owned by: myers
mutable description: Highly flexible 10 amino acid linker. Translates to [GS]_5. Codon-optimize for E. coli, yeast, mammalian.
discontinued: false
dominant: true
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: * gene synthesis * codon optimized for E. coli * experimental distance distributions are described in Neuweiler et al. 2006 papers.
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J18922_sequence/1

Lov2
type: ComponentDefinition
persistent identity: BBa J18927
display id: BBa_J18927
version: 1
was generated by: iGEM to SBOL conversion
description: phot1-LOV2
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: literature gene synthesis
owned by: james
owned by: myers
mutable description: The second LOV domain of Arabidopsis phototropin 1 shows light-induced transient (30s) homo-dimerization between a ground state and an excited monomer (Nakasone et al. 2006). LOV2 is predominantly monomeric at concentrations below 100microM but turns dimeric above. Funnily, the high-conc. dimer state experiences photo-dissociation instead. Either the excitation follows a different course from the dimer starting state or the excitation event itself pushes the complex apart. (CRG-internal ID: rg0090) ===References=== * Nakasone et al. 2006 PMID: 16679373 * Freddolino, Peter L.; Dittrich, Markus; Schulten, Klaus (2006) Dynamic Switching Mechanisms in LOV1 and LOV2 Domains of Plant Phototropins PMID: 16935961
discontinued: false
dominant: false
experience: experience/None
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The fragment used by Nakasone et al. is 475K - 578G. The core domain 449E - 586R has reportedly lower stability at RT (aggregation). * gene synthesis * codon optimized for E. coli
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J18927_sequence/1

mCitrine
type: ComponentDefinition
persistent identity: BBa J18931
display id: BBa_J18931
version: 1
was generated by: iGEM to SBOL conversion
description: mCitrine
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: gene synthesis
owned by: james
owned by: myers
mutable description: Yellow fluorescent protein mCitrine. ===See also=== * BBa_J18930 ===References=== * Griesbeck O, Baird GS, Campbell RE, Zacharias DA, Tsien RY. (2001) Reducing the environmental sensitivity of yellow fluorescent protein. Mechanism and applications. PMID: 11387331
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: * starts with consensus VSKGEEL * ends with consensus DELYK
star: false
creator: Raik Gruenberg
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_J18931_sequence/1

HisTag
type: ComponentDefinition
persistent identity: BBa J18909
display id: BBa_J18909
version: 1
was generated by: iGEM to SBOL conversion
description: His tag (6xHis; E. coli - optimized)
created: 2010-01-15T12:00:00Z
modified: 2015-08-31T04:08:35Z
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
mutable description: Hexahistidine affinity tag. Binds zinc, nickel, or cobalt affinity resins. =Notes= This part is identical to BBa_I757013 (which does not seem to be available though). =References= Wikipedia article: [http://en.wikipedia.org/wiki/Polyhistidine-tag]
discontinued: false
dominant: false
experience: experience/None
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Simple repetition of the His codon most frequent in E. coli -- a more balanced codon-choice may be better.
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: PolyHis
sequence: https://synbiohub.org/public/igem/BBa_J18909_sequence/1

PolyHis
type: SequenceAnnotation
persistent identity: annotation2065463
display id: annotation2065463
version: 1
top level: HisTag
location: https://synbiohub.org/public/igem/BBa_J18909/annotation2065463/range2065463/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_J18909/annotation2065463/range2065463/1
type: Range
persistent identity: range2065463
display id: range2065463
version: 1
top level: HisTag
direction: direction/forward
start: 1
end: 18
orientation: inline

T7stop
type: ComponentDefinition
persistent identity: BBa J18913
display id: BBa_J18913
version: 1
was generated by: iGEM to SBOL conversion
description: T7 terminator (incl. STOP)
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: pET3a expression plasmid
owned by: james
owned by: myers
mutable description: T7 terminator for protein expression in E. coli or in vitro. Part is provided in R.F.C. 25 assembly format.
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: * taken from pET3a expression plasmid * added two STOP to 5' * conservative choice of part boundary, could probably be shortened
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_J18913_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2065587
display id: annotation2065587
version: 1
top level: T7stop
location: https://synbiohub.org/public/igem/BBa_J18913/annotation2065587/range2065587/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_J18913/annotation2065587/range2065587/1
type: Range
persistent identity: range2065587
display id: range2065587
version: 1
top level: T7stop
direction: direction/forward
start: 1
end: 6
orientation: inline

https://synbiohub.org/public/igem/BBa_J18950_sequence/1
type: Sequence
persistent identity: BBa J18950 sequence
display id: BBa_J18950_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagaccacaacggtttccctctagcaataattttgtttaactttaagaaggagatatacatatgaccggcgtgagcaaaggcgaagaactgtttaccggcgtggtgccgattctggtggaactggatggtgatgtgaacggccataaatttagcgtgagcggcgaaggcgaaggtgatgcgacctatggcaaactgaccctgaaatttatttgcaccacgggtaaactgccggttccgtggccgaccctggtgaccacctttggctatggcctgatgtgctttgcgcgttatccggatcatatgaaacagcacgatttctttaaaagcgccatgccggaaggctatgtgcaggaacgcaccatcttttttaaagatgatggcaactataaaacccgtgcggaagtgaaatttgaaggcgataccctggtgaaccgtattgaactgaaaggcatcgatttcaaagaagatggcaacattctgggccataaactggaatataactacaacagccataacgtgtatatcatggcggataaacagaaaaacggcatcaaagtgaactttaaaatccgccacaacattgaagatggcagcgtgcagctggccgatcattatcagcagaacaccccgattggtgatggcccggtgctgctgccggataaccattatctgagctaccagagcaaactgagcaaagatccgaacgaaaaacgtgatcacatggtgctgctggaatttgtgaccgcagccggtattaccctgggcatggatgaactgtacaaaaccggcggtagcggcagcggtagcggtagcggcagcaccggcgaaagcgtggatgataaagtgcgtcagaaagaaatgcgtaaaggcattgatctggccaccaccctggaacgtatcgaaaaaaacttcgtgattaccgatccgcgtctgccggataatccgattatttttgcgagcgatagctttctggaactgaccgaatatagccgtgaagaaattctgggccgtaactgccgttttctgcaaggcccggaaaccgatctgaccaccgtgaaaaaaattcgcaacgcgattgataaccagaccgaagtgaccgtgcagctgattaactataccaaaagcggcaaaaaattctggaacatctttcatctgcaaccgatgcgtgatcagaaaggcgaagtgcagtattttattggcgtgcagctggatggcagcaaacatgtggagccggtgcgtaccggcctggaagtgctgtttcagggcccgaccggccatcatcatcatcatcataccggctagtgactgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0105_sequence/1
type: Sequence
persistent identity: BBa B0105 sequence
display id: BBa_B0105_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: accggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18912_sequence/1
type: Sequence
persistent identity: BBa J18912 sequence
display id: BBa_J18912_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagaccacaacggtttccctctagcaataattttgtttaactttaagaaggagatatacatatg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18919_sequence/1
type: Sequence
persistent identity: BBa J18919 sequence
display id: BBa_J18919_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctggaagtgctgtttcagggcccg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18922_sequence/1
type: Sequence
persistent identity: BBa J18922 sequence
display id: BBa_J18922_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtagcggcagcggtagcggtagcggcagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18927_sequence/1
type: Sequence
persistent identity: BBa J18927 sequence
display id: BBa_J18927_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaaagcgtggatgataaagtgcgtcagaaagaaatgcgtaaaggcattgatctggccaccaccctggaacgtatcgaaaaaaacttcgtgattaccgatccgcgtctgccggataatccgattatttttgcgagcgatagctttctggaactgaccgaatatagccgtgaagaaattctgggccgtaactgccgttttctgcaaggcccggaaaccgatctgaccaccgtgaaaaaaattcgcaacgcgattgataaccagaccgaagtgaccgtgcagctgattaactataccaaaagcggcaaaaaattctggaacatctttcatctgcaaccgatgcgtgatcagaaaggcgaagtgcagtattttattggcgtgcagctggatggcagcaaacatgtggagccggtgcgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18931_sequence/1
type: Sequence
persistent identity: BBa J18931 sequence
display id: BBa_J18931_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtgagcaaaggcgaagaactgtttaccggcgtggtgccgattctggtggaactggatggtgatgtgaacggccataaatttagcgtgagcggcgaaggcgaaggtgatgcgacctatggcaaactgaccctgaaatttatttgcaccacgggtaaactgccggttccgtggccgaccctggtgaccacctttggctatggcctgatgtgctttgcgcgttatccggatcatatgaaacagcacgatttctttaaaagcgccatgccggaaggctatgtgcaggaacgcaccatcttttttaaagatgatggcaactataaaacccgtgcggaagtgaaatttgaaggcgataccctggtgaaccgtattgaactgaaaggcatcgatttcaaagaagatggcaacattctgggccataaactggaatataactacaacagccataacgtgtatatcatggcggataaacagaaaaacggcatcaaagtgaactttaaaatccgccacaacattgaagatggcagcgtgcagctggccgatcattatcagcagaacaccccgattggtgatggcccggtgctgctgccggataaccattatctgagctaccagagcaaactgagcaaagatccgaacgaaaaacgtgatcacatggtgctgctggaatttgtgaccgcagccggtattaccctgggcatggatgaactgtacaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18909_sequence/1
type: Sequence
persistent identity: BBa J18909 sequence
display id: BBa_J18909_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: catcatcatcatcatcat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18913_sequence/1
type: Sequence
persistent identity: BBa J18913 sequence
display id: BBa_J18913_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tagtgactgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggat
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z