BBa_J18951
type: ComponentDefinition
persistent identity: BBa J18951
display id: BBa_J18951
version: 1
was generated by: iGEM to SBOL conversion
description: FRB ~ mCitrine
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: synthetic
owned by: james
owned by: myers
mutable description: P19 expression cassette from Gruenberg et al. 2010 series of interaction reporter proteins. All proteins share the same architecture: * T7Start-domain1-5xGS-domain2-preSCsite-HisTag-T7Stop Only Domains1 and 2 vary and are taken from a list of protein interaction input and output (readout) parts. ===Expression=== Proteins were expressed in E. coli BL21 using T7 Polymerase and were purified by His-tag affinity. ===Reference=== * Gruenberg, Ferrar, van der Sloot, Serrano (2010): Building Blocks for Protein Interaction Devices
discontinued: false
dominant: false
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructs were assembled according to R.F.C. 25. All parts were codon optimized for E. coli.
star: false
creator: Raik Gruenberg
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_J18912
component: BBa_B0105
component: BBa_J18926
component: BBa_J18922
component: BBa_J18909
component: BBa_J18913
component: BBa_J18931
component: BBa_J18919
sequence annotation: BBa_J18912
sequence annotation: BBa_B0105
sequence annotation: BBa_J18926
sequence annotation: BBa_J18922
sequence annotation: BBa_J18931
sequence annotation: BBa_J18919
sequence annotation: BBa_J18909
sequence annotation: BBa_J18913
sequence: https://synbiohub.org/public/igem/BBa_J18951_sequence/1

BBa_J18912
type: Component
persistent identity: component2066782
display id: component2066782
version: 1
top level: BBa_J18951
definition: T7start
access: public

BBa_B0105
type: Component
persistent identity: component2066787
display id: component2066787
version: 1
top level: BBa_J18951
definition: Scar 25
access: public

BBa_J18926
type: Component
persistent identity: component2066785
display id: component2066785
version: 1
top level: BBa_J18951
definition: FRB
access: public

BBa_B0105
type: Component
persistent identity: component2066790
display id: component2066790
version: 1
top level: BBa_J18951
definition: Scar 25
access: public

BBa_J18922
type: Component
persistent identity: component2066788
display id: component2066788
version: 1
top level: BBa_J18951
definition: 5xGS
access: public

BBa_B0105
type: Component
persistent identity: component2066800
display id: component2066800
version: 1
top level: BBa_J18951
definition: Scar 25
access: public

BBa_J18909
type: Component
persistent identity: component2066798
display id: component2066798
version: 1
top level: BBa_J18951
definition: HisTag
access: public

BBa_J18913
type: Component
persistent identity: component2066802
display id: component2066802
version: 1
top level: BBa_J18951
definition: T7stop
access: public

BBa_B0105
type: Component
persistent identity: component2066793
display id: component2066793
version: 1
top level: BBa_J18951
definition: Scar 25
access: public

BBa_J18931
type: Component
persistent identity: component2066791
display id: component2066791
version: 1
top level: BBa_J18951
definition: mCitrine
access: public

BBa_B0105
type: Component
persistent identity: component2066796
display id: component2066796
version: 1
top level: BBa_J18951
definition: Scar 25
access: public

BBa_B0105
type: Component
persistent identity: component2066784
display id: component2066784
version: 1
top level: BBa_J18951
definition: Scar 25
access: public

BBa_J18919
type: Component
persistent identity: component2066794
display id: component2066794
version: 1
top level: BBa_J18951
definition: preSCsite
access: public

BBa_J18912
type: SequenceAnnotation
persistent identity: annotation2066782
display id: annotation2066782
version: 1
top level: BBa_J18951
location: https://synbiohub.org/public/igem/BBa_J18951/annotation2066782/range2066782/1
component: BBa_J18912

https://synbiohub.org/public/igem/BBa_J18951/annotation2066782/range2066782/1
type: Range
persistent identity: range2066782
display id: range2066782
version: 1
top level: BBa_J18951
direction: direction/reverse
start: 1
end: 83
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066784
display id: annotation2066784
version: 1
top level: BBa_J18951
location: https://synbiohub.org/public/igem/BBa_J18951/annotation2066784/range2066784/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18951/annotation2066784/range2066784/1
type: Range
persistent identity: range2066784
display id: range2066784
version: 1
top level: BBa_J18951
direction: direction/reverse
start: 84
end: 89
orientation: inline

BBa_J18926
type: SequenceAnnotation
persistent identity: annotation2066785
display id: annotation2066785
version: 1
top level: BBa_J18951
location: https://synbiohub.org/public/igem/BBa_J18951/annotation2066785/range2066785/1
component: BBa_J18926

https://synbiohub.org/public/igem/BBa_J18951/annotation2066785/range2066785/1
type: Range
persistent identity: range2066785
display id: range2066785
version: 1
top level: BBa_J18951
direction: direction/reverse
start: 90
end: 368
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066787
display id: annotation2066787
version: 1
top level: BBa_J18951
location: https://synbiohub.org/public/igem/BBa_J18951/annotation2066787/range2066787/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18951/annotation2066787/range2066787/1
type: Range
persistent identity: range2066787
display id: range2066787
version: 1
top level: BBa_J18951
direction: direction/reverse
start: 369
end: 374
orientation: inline

BBa_J18922
type: SequenceAnnotation
persistent identity: annotation2066788
display id: annotation2066788
version: 1
top level: BBa_J18951
location: https://synbiohub.org/public/igem/BBa_J18951/annotation2066788/range2066788/1
component: BBa_J18922

https://synbiohub.org/public/igem/BBa_J18951/annotation2066788/range2066788/1
type: Range
persistent identity: range2066788
display id: range2066788
version: 1
top level: BBa_J18951
direction: direction/reverse
start: 375
end: 404
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066790
display id: annotation2066790
version: 1
top level: BBa_J18951
location: https://synbiohub.org/public/igem/BBa_J18951/annotation2066790/range2066790/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18951/annotation2066790/range2066790/1
type: Range
persistent identity: range2066790
display id: range2066790
version: 1
top level: BBa_J18951
direction: direction/reverse
start: 405
end: 410
orientation: inline

BBa_J18931
type: SequenceAnnotation
persistent identity: annotation2066791
display id: annotation2066791
version: 1
top level: BBa_J18951
location: https://synbiohub.org/public/igem/BBa_J18951/annotation2066791/range2066791/1
component: BBa_J18931

https://synbiohub.org/public/igem/BBa_J18951/annotation2066791/range2066791/1
type: Range
persistent identity: range2066791
display id: range2066791
version: 1
top level: BBa_J18951
direction: direction/reverse
start: 411
end: 1124
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066793
display id: annotation2066793
version: 1
top level: BBa_J18951
location: https://synbiohub.org/public/igem/BBa_J18951/annotation2066793/range2066793/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18951/annotation2066793/range2066793/1
type: Range
persistent identity: range2066793
display id: range2066793
version: 1
top level: BBa_J18951
direction: direction/reverse
start: 1125
end: 1130
orientation: inline

BBa_J18919
type: SequenceAnnotation
persistent identity: annotation2066794
display id: annotation2066794
version: 1
top level: BBa_J18951
location: https://synbiohub.org/public/igem/BBa_J18951/annotation2066794/range2066794/1
component: BBa_J18919

https://synbiohub.org/public/igem/BBa_J18951/annotation2066794/range2066794/1
type: Range
persistent identity: range2066794
display id: range2066794
version: 1
top level: BBa_J18951
direction: direction/reverse
start: 1131
end: 1154
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066796
display id: annotation2066796
version: 1
top level: BBa_J18951
location: https://synbiohub.org/public/igem/BBa_J18951/annotation2066796/range2066796/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18951/annotation2066796/range2066796/1
type: Range
persistent identity: range2066796
display id: range2066796
version: 1
top level: BBa_J18951
direction: direction/reverse
start: 1155
end: 1160
orientation: inline

BBa_J18909
type: SequenceAnnotation
persistent identity: annotation2066798
display id: annotation2066798
version: 1
top level: BBa_J18951
location: https://synbiohub.org/public/igem/BBa_J18951/annotation2066798/range2066798/1
component: BBa_J18909

https://synbiohub.org/public/igem/BBa_J18951/annotation2066798/range2066798/1
type: Range
persistent identity: range2066798
display id: range2066798
version: 1
top level: BBa_J18951
direction: direction/reverse
start: 1161
end: 1178
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066800
display id: annotation2066800
version: 1
top level: BBa_J18951
location: https://synbiohub.org/public/igem/BBa_J18951/annotation2066800/range2066800/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18951/annotation2066800/range2066800/1
type: Range
persistent identity: range2066800
display id: range2066800
version: 1
top level: BBa_J18951
direction: direction/reverse
start: 1179
end: 1184
orientation: inline

BBa_J18913
type: SequenceAnnotation
persistent identity: annotation2066802
display id: annotation2066802
version: 1
top level: BBa_J18951
location: https://synbiohub.org/public/igem/BBa_J18951/annotation2066802/range2066802/1
component: BBa_J18913

https://synbiohub.org/public/igem/BBa_J18951/annotation2066802/range2066802/1
type: Range
persistent identity: range2066802
display id: range2066802
version: 1
top level: BBa_J18951
direction: direction/reverse
start: 1185
end: 1319
orientation: inline

Scar 25
type: ComponentDefinition
persistent identity: BBa B0105
display id: BBa_B0105
version: 1
was generated by: iGEM to SBOL conversion
description: RFC 25 Scar Sequence
created: 2009-10-14T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: BBF RFC 25
owned by: james
owned by: myers
mutable description: This is the scar produced by assembly using RFC 25. If you are assembling a composite part using RFC 25, you can insert this part and specify blunt assembly to get the desired sequence.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 25
owning group id: 397
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Simple DNA sequence
star: false
creator: Randy Rettberg
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: Scar 25
sequence: https://synbiohub.org/public/igem/BBa_B0105_sequence/1

Scar 25
type: SequenceAnnotation
persistent identity: annotation2041621
display id: annotation2041621
version: 1
top level: Scar 25
location: https://synbiohub.org/public/igem/BBa_B0105/annotation2041621/range2041621/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0105/annotation2041621/range2041621/1
type: Range
persistent identity: range2041621
display id: range2041621
version: 1
top level: Scar 25
direction: direction/forward
start: 1
end: 6
orientation: inline

T7start
type: ComponentDefinition
persistent identity: BBa J18912
display id: BBa_J18912
version: 1
was generated by: iGEM to SBOL conversion
description: T7 promoter + RBS + START
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: pET3a expression vector
owned by: james
owned by: myers
mutable description: Strong T7 promoter and RBS including start codon for protein expression in E. coli or in-vitro. Part is provided in RFC 25 assembly format.
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: * sequence is taken from the commonly used pET3a expression vector * deleted XbaI site between prom. and RBS * T7 promoter sequence (1-19) equals the one in BBa_I712074 * Conservative choice of part boundaries and modifications -- the part's size could probably be reduced.
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_J18912_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2065586
display id: annotation2065586
version: 1
top level: T7start
location: https://synbiohub.org/public/igem/BBa_J18912/annotation2065586/range2065586/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_J18912/annotation2065586/range2065586/1
type: Range
persistent identity: range2065586
display id: range2065586
version: 1
top level: T7start
direction: direction/forward
start: 81
end: 83
orientation: inline

preSCsite
type: ComponentDefinition
persistent identity: BBa J18919
display id: BBa_J18919
version: 1
was generated by: iGEM to SBOL conversion
description: preScission protease cleavage site (E. coli-optimized)
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: gene synthesis
owned by: james
owned by: myers
mutable description: cleavage site for PreScission??? protease. preScission protease cleavage site (PreScission??? protease is a genetically engineered form of human rhinovirus 3C protease with a GST fusion, allowing for facile cleavage and purification of GST-tagged proteins along with protease removal after recombinant protein cleavage. Enables low-temperature cleavage of fusion proteins containing the eight-residue recognition sequence.) cleavage site: * Leu-Glu-Val-Leu-Phe-Gln↓Gly-Pro References ============== Walker et al. (1994): Efficient and rapid affinity purification of proteins using recombinant fusion proteases. PMID: 7764949
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: * gene synthesis * codon optimized for E. coli
star: false
creator: Raik Gruenberg
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_J18919_sequence/1

5xGS
type: ComponentDefinition
persistent identity: BBa J18922
display id: BBa_J18922
version: 1
was generated by: iGEM to SBOL conversion
description: 10aa [GS]x linker
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: gene synthesis
owned by: james
owned by: myers
mutable description: Highly flexible 10 amino acid linker. Translates to [GS]_5. Codon-optimize for E. coli, yeast, mammalian.
discontinued: false
dominant: true
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: * gene synthesis * codon optimized for E. coli * experimental distance distributions are described in Neuweiler et al. 2006 papers.
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J18922_sequence/1

FRB
type: ComponentDefinition
persistent identity: BBa J18926
display id: BBa_J18926
version: 1
was generated by: iGEM to SBOL conversion
description: FRB(T2098L)
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: human mTOR (aa sequence) gene synthesis
owned by: james
owned by: myers
mutable description: Modified FKBP12-rapamycin-binding domain (FRB) of human mTOR Kinase (=mammalian Target of Rapamycin, also called FRAP = FKBP12-rapamycin associated protein). In presence of the immunosupressant drug rapamycin, FRB forms a high-affinity complex with FKBP12. There is no detectable FKBP12 - FRB interaction in absence of rapamycin. ===Modification for a non-toxic dimerizer=== Rapamycin arrests growth and proliferation of many cell types (reviewed in Jacinto and Hall, 2003) by inhibiting protein translation. The mutation T2089L (mTor numbering) makes FRB binding to a non-toxic Rapamycin analogue (rapalogue) AP21967 as well as the original Rapamycin. The FRB - FKBP12 heterodimerization can then be triggered by both Rapamycin and the non-toxic rapalogue. This variant of FRB and the synthetic dimerizer AP21967 are the basis of the Argent(tm) heterodimerization kit from Ariad Pharmaceuticals Inc. '''Note:''' Two additional mutations would convert FRB into FRB* which is used for conditional (drug-reversible) degradation of fusion proteins. These two additional mutations are not implemented in this part. ===Protein Parameters:=== * Number of amino acids: 93 * Molecular weight: 11285.9 * Theoretical pI: 6.47
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: * aa translation from human mTOR, * mutation T2089L (verified against pC4-RHE from Ariad) * GeneArt codon optimization for E. coli * gene synthesis
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J18926_sequence/1

mCitrine
type: ComponentDefinition
persistent identity: BBa J18931
display id: BBa_J18931
version: 1
was generated by: iGEM to SBOL conversion
description: mCitrine
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: gene synthesis
owned by: james
owned by: myers
mutable description: Yellow fluorescent protein mCitrine. ===See also=== * BBa_J18930 ===References=== * Griesbeck O, Baird GS, Campbell RE, Zacharias DA, Tsien RY. (2001) Reducing the environmental sensitivity of yellow fluorescent protein. Mechanism and applications. PMID: 11387331
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: * starts with consensus VSKGEEL * ends with consensus DELYK
star: false
creator: Raik Gruenberg
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_J18931_sequence/1

HisTag
type: ComponentDefinition
persistent identity: BBa J18909
display id: BBa_J18909
version: 1
was generated by: iGEM to SBOL conversion
description: His tag (6xHis; E. coli - optimized)
created: 2010-01-15T12:00:00Z
modified: 2015-08-31T04:08:35Z
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
mutable description: Hexahistidine affinity tag. Binds zinc, nickel, or cobalt affinity resins. =Notes= This part is identical to BBa_I757013 (which does not seem to be available though). =References= Wikipedia article: [http://en.wikipedia.org/wiki/Polyhistidine-tag]
discontinued: false
dominant: false
experience: experience/None
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Simple repetition of the His codon most frequent in E. coli -- a more balanced codon-choice may be better.
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: PolyHis
sequence: https://synbiohub.org/public/igem/BBa_J18909_sequence/1

PolyHis
type: SequenceAnnotation
persistent identity: annotation2065463
display id: annotation2065463
version: 1
top level: HisTag
location: https://synbiohub.org/public/igem/BBa_J18909/annotation2065463/range2065463/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_J18909/annotation2065463/range2065463/1
type: Range
persistent identity: range2065463
display id: range2065463
version: 1
top level: HisTag
direction: direction/forward
start: 1
end: 18
orientation: inline

T7stop
type: ComponentDefinition
persistent identity: BBa J18913
display id: BBa_J18913
version: 1
was generated by: iGEM to SBOL conversion
description: T7 terminator (incl. STOP)
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: pET3a expression plasmid
owned by: james
owned by: myers
mutable description: T7 terminator for protein expression in E. coli or in vitro. Part is provided in R.F.C. 25 assembly format.
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: * taken from pET3a expression plasmid * added two STOP to 5' * conservative choice of part boundary, could probably be shortened
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_J18913_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2065587
display id: annotation2065587
version: 1
top level: T7stop
location: https://synbiohub.org/public/igem/BBa_J18913/annotation2065587/range2065587/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_J18913/annotation2065587/range2065587/1
type: Range
persistent identity: range2065587
display id: range2065587
version: 1
top level: T7stop
direction: direction/forward
start: 1
end: 6
orientation: inline

https://synbiohub.org/public/igem/BBa_J18951_sequence/1
type: Sequence
persistent identity: BBa J18951 sequence
display id: BBa_J18951_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagaccacaacggtttccctctagcaataattttgtttaactttaagaaggagatatacatatgaccggcatcctgtggcatgaaatgtggcatgaaggcctggaagaagcgagccgtctgtattttggcgaacgtaacgtgaaaggcatgtttgaagtgctggaaccgctgcatgccatgatggaacgtggcccgcagaccctgaaagaaaccagctttaaccaggcgtatggccgtgatctgatggaagcgcaggaatggtgccgtaaatacatgaaaagcggcaacgtgaaagatctgctgcaagcgtgggatctgtattatcatgtgtttcgccgcatcagcaaaaccggcggtagcggcagcggtagcggtagcggcagcaccggcgtgagcaaaggcgaagaactgtttaccggcgtggtgccgattctggtggaactggatggtgatgtgaacggccataaatttagcgtgagcggcgaaggcgaaggtgatgcgacctatggcaaactgaccctgaaatttatttgcaccacgggtaaactgccggttccgtggccgaccctggtgaccacctttggctatggcctgatgtgctttgcgcgttatccggatcatatgaaacagcacgatttctttaaaagcgccatgccggaaggctatgtgcaggaacgcaccatcttttttaaagatgatggcaactataaaacccgtgcggaagtgaaatttgaaggcgataccctggtgaaccgtattgaactgaaaggcatcgatttcaaagaagatggcaacattctgggccataaactggaatataactacaacagccataacgtgtatatcatggcggataaacagaaaaacggcatcaaagtgaactttaaaatccgccacaacattgaagatggcagcgtgcagctggccgatcattatcagcagaacaccccgattggtgatggcccggtgctgctgccggataaccattatctgagctaccagagcaaactgagcaaagatccgaacgaaaaacgtgatcacatggtgctgctggaatttgtgaccgcagccggtattaccctgggcatggatgaactgtacaaaaccggcctggaagtgctgtttcagggcccgaccggccatcatcatcatcatcataccggctagtgactgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0105_sequence/1
type: Sequence
persistent identity: BBa B0105 sequence
display id: BBa_B0105_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: accggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18912_sequence/1
type: Sequence
persistent identity: BBa J18912 sequence
display id: BBa_J18912_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagaccacaacggtttccctctagcaataattttgtttaactttaagaaggagatatacatatg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18919_sequence/1
type: Sequence
persistent identity: BBa J18919 sequence
display id: BBa_J18919_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctggaagtgctgtttcagggcccg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18922_sequence/1
type: Sequence
persistent identity: BBa J18922 sequence
display id: BBa_J18922_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtagcggcagcggtagcggtagcggcagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18926_sequence/1
type: Sequence
persistent identity: BBa J18926 sequence
display id: BBa_J18926_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atcctgtggcatgaaatgtggcatgaaggcctggaagaagcgagccgtctgtattttggcgaacgtaacgtgaaaggcatgtttgaagtgctggaaccgctgcatgccatgatggaacgtggcccgcagaccctgaaagaaaccagctttaaccaggcgtatggccgtgatctgatggaagcgcaggaatggtgccgtaaatacatgaaaagcggcaacgtgaaagatctgctgcaagcgtgggatctgtattatcatgtgtttcgccgcatcagcaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18931_sequence/1
type: Sequence
persistent identity: BBa J18931 sequence
display id: BBa_J18931_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtgagcaaaggcgaagaactgtttaccggcgtggtgccgattctggtggaactggatggtgatgtgaacggccataaatttagcgtgagcggcgaaggcgaaggtgatgcgacctatggcaaactgaccctgaaatttatttgcaccacgggtaaactgccggttccgtggccgaccctggtgaccacctttggctatggcctgatgtgctttgcgcgttatccggatcatatgaaacagcacgatttctttaaaagcgccatgccggaaggctatgtgcaggaacgcaccatcttttttaaagatgatggcaactataaaacccgtgcggaagtgaaatttgaaggcgataccctggtgaaccgtattgaactgaaaggcatcgatttcaaagaagatggcaacattctgggccataaactggaatataactacaacagccataacgtgtatatcatggcggataaacagaaaaacggcatcaaagtgaactttaaaatccgccacaacattgaagatggcagcgtgcagctggccgatcattatcagcagaacaccccgattggtgatggcccggtgctgctgccggataaccattatctgagctaccagagcaaactgagcaaagatccgaacgaaaaacgtgatcacatggtgctgctggaatttgtgaccgcagccggtattaccctgggcatggatgaactgtacaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18909_sequence/1
type: Sequence
persistent identity: BBa J18909 sequence
display id: BBa_J18909_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: catcatcatcatcatcat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18913_sequence/1
type: Sequence
persistent identity: BBa J18913 sequence
display id: BBa_J18913_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tagtgactgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggat
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z