BBa_J18952
type: ComponentDefinition
persistent identity: BBa J18952
display id: BBa_J18952
version: 1
was generated by: iGEM to SBOL conversion
description: FRB ~ mCerulean
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: synthetic
owned by: james
owned by: myers
mutable description: P20 expression cassette from Gruenberg et al. 2010 series of interaction reporter proteins. All proteins share the same architecture: * T7Start-domain1-5xGS-domain2-preSCsite-HisTag-T7Stop Only Domains1 and 2 vary and are taken from a list of protein interaction input and output (readout) parts. ===Expression=== Proteins were expressed in E. coli BL21 using T7 Polymerase and were purified by His-tag affinity. ===Reference=== * Gruenberg, Ferrar, van der Sloot, Serrano (2010): Building Blocks for Protein Interaction Devices
discontinued: false
dominant: false
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructs were assembled according to R.F.C. 25. All parts were codon optimized for E. coli.
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
component: BBa_J18912
component: BBa_J18926
component: BBa_B0105
component: BBa_J18922
component: BBa_J18930
component: BBa_J18919
component: BBa_J18909
component: BBa_J18913
sequence annotation: BBa_J18912
sequence annotation: BBa_B0105
sequence annotation: BBa_J18926
sequence annotation: BBa_J18922
sequence annotation: BBa_J18930
sequence annotation: BBa_J18919
sequence annotation: BBa_J18909
sequence annotation: BBa_J18913
sequence: https://synbiohub.org/public/igem/BBa_J18952_sequence/1

BBa_J18912
type: Component
persistent identity: component2066804
display id: component2066804
version: 1
top level: BBa_J18952
definition: T7start
access: public

BBa_J18926
type: Component
persistent identity: component2066807
display id: component2066807
version: 1
top level: BBa_J18952
definition: FRB
access: public

BBa_B0105
type: Component
persistent identity: component2066806
display id: component2066806
version: 1
top level: BBa_J18952
definition: Scar 25
access: public

BBa_J18922
type: Component
persistent identity: component2066810
display id: component2066810
version: 1
top level: BBa_J18952
definition: 5xGS
access: public

BBa_B0105
type: Component
persistent identity: component2066809
display id: component2066809
version: 1
top level: BBa_J18952
definition: Scar 25
access: public

BBa_J18930
type: Component
persistent identity: component2066813
display id: component2066813
version: 1
top level: BBa_J18952
definition: mCerulean
access: public

BBa_B0105
type: Component
persistent identity: component2066812
display id: component2066812
version: 1
top level: BBa_J18952
definition: Scar 25
access: public

BBa_J18919
type: Component
persistent identity: component2066816
display id: component2066816
version: 1
top level: BBa_J18952
definition: preSCsite
access: public

BBa_B0105
type: Component
persistent identity: component2066815
display id: component2066815
version: 1
top level: BBa_J18952
definition: Scar 25
access: public

BBa_J18909
type: Component
persistent identity: component2066820
display id: component2066820
version: 1
top level: BBa_J18952
definition: HisTag
access: public

BBa_B0105
type: Component
persistent identity: component2066818
display id: component2066818
version: 1
top level: BBa_J18952
definition: Scar 25
access: public

BBa_J18913
type: Component
persistent identity: component2066824
display id: component2066824
version: 1
top level: BBa_J18952
definition: T7stop
access: public

BBa_B0105
type: Component
persistent identity: component2066822
display id: component2066822
version: 1
top level: BBa_J18952
definition: Scar 25
access: public

BBa_J18912
type: SequenceAnnotation
persistent identity: annotation2066804
display id: annotation2066804
version: 1
top level: BBa_J18952
location: https://synbiohub.org/public/igem/BBa_J18952/annotation2066804/range2066804/1
component: BBa_J18912

https://synbiohub.org/public/igem/BBa_J18952/annotation2066804/range2066804/1
type: Range
persistent identity: range2066804
display id: range2066804
version: 1
top level: BBa_J18952
direction: direction/reverse
start: 1
end: 83
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066806
display id: annotation2066806
version: 1
top level: BBa_J18952
location: https://synbiohub.org/public/igem/BBa_J18952/annotation2066806/range2066806/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18952/annotation2066806/range2066806/1
type: Range
persistent identity: range2066806
display id: range2066806
version: 1
top level: BBa_J18952
direction: direction/reverse
start: 84
end: 89
orientation: inline

BBa_J18926
type: SequenceAnnotation
persistent identity: annotation2066807
display id: annotation2066807
version: 1
top level: BBa_J18952
location: https://synbiohub.org/public/igem/BBa_J18952/annotation2066807/range2066807/1
component: BBa_J18926

https://synbiohub.org/public/igem/BBa_J18952/annotation2066807/range2066807/1
type: Range
persistent identity: range2066807
display id: range2066807
version: 1
top level: BBa_J18952
direction: direction/reverse
start: 90
end: 368
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066809
display id: annotation2066809
version: 1
top level: BBa_J18952
location: https://synbiohub.org/public/igem/BBa_J18952/annotation2066809/range2066809/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18952/annotation2066809/range2066809/1
type: Range
persistent identity: range2066809
display id: range2066809
version: 1
top level: BBa_J18952
direction: direction/reverse
start: 369
end: 374
orientation: inline

BBa_J18922
type: SequenceAnnotation
persistent identity: annotation2066810
display id: annotation2066810
version: 1
top level: BBa_J18952
location: https://synbiohub.org/public/igem/BBa_J18952/annotation2066810/range2066810/1
component: BBa_J18922

https://synbiohub.org/public/igem/BBa_J18952/annotation2066810/range2066810/1
type: Range
persistent identity: range2066810
display id: range2066810
version: 1
top level: BBa_J18952
direction: direction/reverse
start: 375
end: 404
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066812
display id: annotation2066812
version: 1
top level: BBa_J18952
location: https://synbiohub.org/public/igem/BBa_J18952/annotation2066812/range2066812/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18952/annotation2066812/range2066812/1
type: Range
persistent identity: range2066812
display id: range2066812
version: 1
top level: BBa_J18952
direction: direction/reverse
start: 405
end: 410
orientation: inline

BBa_J18930
type: SequenceAnnotation
persistent identity: annotation2066813
display id: annotation2066813
version: 1
top level: BBa_J18952
location: https://synbiohub.org/public/igem/BBa_J18952/annotation2066813/range2066813/1
component: BBa_J18930

https://synbiohub.org/public/igem/BBa_J18952/annotation2066813/range2066813/1
type: Range
persistent identity: range2066813
display id: range2066813
version: 1
top level: BBa_J18952
direction: direction/reverse
start: 411
end: 1124
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066815
display id: annotation2066815
version: 1
top level: BBa_J18952
location: https://synbiohub.org/public/igem/BBa_J18952/annotation2066815/range2066815/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18952/annotation2066815/range2066815/1
type: Range
persistent identity: range2066815
display id: range2066815
version: 1
top level: BBa_J18952
direction: direction/reverse
start: 1125
end: 1130
orientation: inline

BBa_J18919
type: SequenceAnnotation
persistent identity: annotation2066816
display id: annotation2066816
version: 1
top level: BBa_J18952
location: https://synbiohub.org/public/igem/BBa_J18952/annotation2066816/range2066816/1
component: BBa_J18919

https://synbiohub.org/public/igem/BBa_J18952/annotation2066816/range2066816/1
type: Range
persistent identity: range2066816
display id: range2066816
version: 1
top level: BBa_J18952
direction: direction/reverse
start: 1131
end: 1154
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066818
display id: annotation2066818
version: 1
top level: BBa_J18952
location: https://synbiohub.org/public/igem/BBa_J18952/annotation2066818/range2066818/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18952/annotation2066818/range2066818/1
type: Range
persistent identity: range2066818
display id: range2066818
version: 1
top level: BBa_J18952
direction: direction/reverse
start: 1155
end: 1160
orientation: inline

BBa_J18909
type: SequenceAnnotation
persistent identity: annotation2066820
display id: annotation2066820
version: 1
top level: BBa_J18952
location: https://synbiohub.org/public/igem/BBa_J18952/annotation2066820/range2066820/1
component: BBa_J18909

https://synbiohub.org/public/igem/BBa_J18952/annotation2066820/range2066820/1
type: Range
persistent identity: range2066820
display id: range2066820
version: 1
top level: BBa_J18952
direction: direction/reverse
start: 1161
end: 1178
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066822
display id: annotation2066822
version: 1
top level: BBa_J18952
location: https://synbiohub.org/public/igem/BBa_J18952/annotation2066822/range2066822/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18952/annotation2066822/range2066822/1
type: Range
persistent identity: range2066822
display id: range2066822
version: 1
top level: BBa_J18952
direction: direction/reverse
start: 1179
end: 1184
orientation: inline

BBa_J18913
type: SequenceAnnotation
persistent identity: annotation2066824
display id: annotation2066824
version: 1
top level: BBa_J18952
location: https://synbiohub.org/public/igem/BBa_J18952/annotation2066824/range2066824/1
component: BBa_J18913

https://synbiohub.org/public/igem/BBa_J18952/annotation2066824/range2066824/1
type: Range
persistent identity: range2066824
display id: range2066824
version: 1
top level: BBa_J18952
direction: direction/reverse
start: 1185
end: 1319
orientation: inline

Scar 25
type: ComponentDefinition
persistent identity: BBa B0105
display id: BBa_B0105
version: 1
was generated by: iGEM to SBOL conversion
description: RFC 25 Scar Sequence
created: 2009-10-14T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: BBF RFC 25
owned by: james
owned by: myers
mutable description: This is the scar produced by assembly using RFC 25. If you are assembling a composite part using RFC 25, you can insert this part and specify blunt assembly to get the desired sequence.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 25
owning group id: 397
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Simple DNA sequence
star: false
creator: Randy Rettberg
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: Scar 25
sequence: https://synbiohub.org/public/igem/BBa_B0105_sequence/1

Scar 25
type: SequenceAnnotation
persistent identity: annotation2041621
display id: annotation2041621
version: 1
top level: Scar 25
location: https://synbiohub.org/public/igem/BBa_B0105/annotation2041621/range2041621/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0105/annotation2041621/range2041621/1
type: Range
persistent identity: range2041621
display id: range2041621
version: 1
top level: Scar 25
direction: direction/forward
start: 1
end: 6
orientation: inline

T7start
type: ComponentDefinition
persistent identity: BBa J18912
display id: BBa_J18912
version: 1
was generated by: iGEM to SBOL conversion
description: T7 promoter + RBS + START
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: pET3a expression vector
owned by: james
owned by: myers
mutable description: Strong T7 promoter and RBS including start codon for protein expression in E. coli or in-vitro. Part is provided in RFC 25 assembly format.
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: * sequence is taken from the commonly used pET3a expression vector * deleted XbaI site between prom. and RBS * T7 promoter sequence (1-19) equals the one in BBa_I712074 * Conservative choice of part boundaries and modifications -- the part's size could probably be reduced.
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_J18912_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2065586
display id: annotation2065586
version: 1
top level: T7start
location: https://synbiohub.org/public/igem/BBa_J18912/annotation2065586/range2065586/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_J18912/annotation2065586/range2065586/1
type: Range
persistent identity: range2065586
display id: range2065586
version: 1
top level: T7start
direction: direction/forward
start: 81
end: 83
orientation: inline

preSCsite
type: ComponentDefinition
persistent identity: BBa J18919
display id: BBa_J18919
version: 1
was generated by: iGEM to SBOL conversion
description: preScission protease cleavage site (E. coli-optimized)
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: gene synthesis
owned by: james
owned by: myers
mutable description: cleavage site for PreScission??? protease. preScission protease cleavage site (PreScission??? protease is a genetically engineered form of human rhinovirus 3C protease with a GST fusion, allowing for facile cleavage and purification of GST-tagged proteins along with protease removal after recombinant protein cleavage. Enables low-temperature cleavage of fusion proteins containing the eight-residue recognition sequence.) cleavage site: * Leu-Glu-Val-Leu-Phe-Gln↓Gly-Pro References ============== Walker et al. (1994): Efficient and rapid affinity purification of proteins using recombinant fusion proteases. PMID: 7764949
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: * gene synthesis * codon optimized for E. coli
star: false
creator: Raik Gruenberg
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_J18919_sequence/1

5xGS
type: ComponentDefinition
persistent identity: BBa J18922
display id: BBa_J18922
version: 1
was generated by: iGEM to SBOL conversion
description: 10aa [GS]x linker
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: gene synthesis
owned by: james
owned by: myers
mutable description: Highly flexible 10 amino acid linker. Translates to [GS]_5. Codon-optimize for E. coli, yeast, mammalian.
discontinued: false
dominant: true
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: * gene synthesis * codon optimized for E. coli * experimental distance distributions are described in Neuweiler et al. 2006 papers.
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J18922_sequence/1

FRB
type: ComponentDefinition
persistent identity: BBa J18926
display id: BBa_J18926
version: 1
was generated by: iGEM to SBOL conversion
description: FRB(T2098L)
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: human mTOR (aa sequence) gene synthesis
owned by: james
owned by: myers
mutable description: Modified FKBP12-rapamycin-binding domain (FRB) of human mTOR Kinase (=mammalian Target of Rapamycin, also called FRAP = FKBP12-rapamycin associated protein). In presence of the immunosupressant drug rapamycin, FRB forms a high-affinity complex with FKBP12. There is no detectable FKBP12 - FRB interaction in absence of rapamycin. ===Modification for a non-toxic dimerizer=== Rapamycin arrests growth and proliferation of many cell types (reviewed in Jacinto and Hall, 2003) by inhibiting protein translation. The mutation T2089L (mTor numbering) makes FRB binding to a non-toxic Rapamycin analogue (rapalogue) AP21967 as well as the original Rapamycin. The FRB - FKBP12 heterodimerization can then be triggered by both Rapamycin and the non-toxic rapalogue. This variant of FRB and the synthetic dimerizer AP21967 are the basis of the Argent(tm) heterodimerization kit from Ariad Pharmaceuticals Inc. '''Note:''' Two additional mutations would convert FRB into FRB* which is used for conditional (drug-reversible) degradation of fusion proteins. These two additional mutations are not implemented in this part. ===Protein Parameters:=== * Number of amino acids: 93 * Molecular weight: 11285.9 * Theoretical pI: 6.47
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: * aa translation from human mTOR, * mutation T2089L (verified against pC4-RHE from Ariad) * GeneArt codon optimization for E. coli * gene synthesis
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J18926_sequence/1

mCerulean
type: ComponentDefinition
persistent identity: BBa J18930
display id: BBa_J18930
version: 1
was generated by: iGEM to SBOL conversion
description: mCerulean CFP
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: literature gene synthesis
owned by: james
owned by: myers
mutable description: Cerulean GFP is an optimized FRET donor molecule that was rationally designed by Piston and co-workers to eliminate the excited-state heterogeneity of ECFP. In ECFP, a single-exponential fit to the fluorescence lifetime is not feasible due to multiple components, whereas Cerulean exhibits essentially homogeneous excited-state decay kinetics, rendering this protein useful for lifetime imaging... Live cell imaging experiments have demonstrated that Cerulean GFP undergoes highly efficient FRET to yellow acceptor molecules (25, 26). Additionally, Cerulean exhibits more than twice the brightness of ECFP and CyPet, emitting light at fluorescence intensities similar to Citrine (20). ===References=== * Rizzo MA, Springer GH, Granada B, Piston DW. (2004) An improved cyan fluorescent protein variant useful for FRET. PMID: 14990965
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: * amino acid sequence taken from a pAG304GAL-Cerulean-ccdB from LindQuist lab * borders trimmed to match the construct used for PDB 2Q57 (last 9 AA are not resolved in PDB) * structure starts with MSK, this construct starts with MVSK but the M has been skipped for this Biobrick * gene synthesis * codon optimized for E. coli
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J18930_sequence/1

HisTag
type: ComponentDefinition
persistent identity: BBa J18909
display id: BBa_J18909
version: 1
was generated by: iGEM to SBOL conversion
description: His tag (6xHis; E. coli - optimized)
created: 2010-01-15T12:00:00Z
modified: 2015-08-31T04:08:35Z
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
mutable description: Hexahistidine affinity tag. Binds zinc, nickel, or cobalt affinity resins. =Notes= This part is identical to BBa_I757013 (which does not seem to be available though). =References= Wikipedia article: [http://en.wikipedia.org/wiki/Polyhistidine-tag]
discontinued: false
dominant: false
experience: experience/None
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Simple repetition of the His codon most frequent in E. coli -- a more balanced codon-choice may be better.
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: PolyHis
sequence: https://synbiohub.org/public/igem/BBa_J18909_sequence/1

PolyHis
type: SequenceAnnotation
persistent identity: annotation2065463
display id: annotation2065463
version: 1
top level: HisTag
location: https://synbiohub.org/public/igem/BBa_J18909/annotation2065463/range2065463/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_J18909/annotation2065463/range2065463/1
type: Range
persistent identity: range2065463
display id: range2065463
version: 1
top level: HisTag
direction: direction/forward
start: 1
end: 18
orientation: inline

T7stop
type: ComponentDefinition
persistent identity: BBa J18913
display id: BBa_J18913
version: 1
was generated by: iGEM to SBOL conversion
description: T7 terminator (incl. STOP)
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: pET3a expression plasmid
owned by: james
owned by: myers
mutable description: T7 terminator for protein expression in E. coli or in vitro. Part is provided in R.F.C. 25 assembly format.
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: * taken from pET3a expression plasmid * added two STOP to 5' * conservative choice of part boundary, could probably be shortened
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_J18913_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2065587
display id: annotation2065587
version: 1
top level: T7stop
location: https://synbiohub.org/public/igem/BBa_J18913/annotation2065587/range2065587/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_J18913/annotation2065587/range2065587/1
type: Range
persistent identity: range2065587
display id: range2065587
version: 1
top level: T7stop
direction: direction/forward
start: 1
end: 6
orientation: inline

https://synbiohub.org/public/igem/BBa_J18952_sequence/1
type: Sequence
persistent identity: BBa J18952 sequence
display id: BBa_J18952_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagaccacaacggtttccctctagcaataattttgtttaactttaagaaggagatatacatatgaccggcatcctgtggcatgaaatgtggcatgaaggcctggaagaagcgagccgtctgtattttggcgaacgtaacgtgaaaggcatgtttgaagtgctggaaccgctgcatgccatgatggaacgtggcccgcagaccctgaaagaaaccagctttaaccaggcgtatggccgtgatctgatggaagcgcaggaatggtgccgtaaatacatgaaaagcggcaacgtgaaagatctgctgcaagcgtgggatctgtattatcatgtgtttcgccgcatcagcaaaaccggcggtagcggcagcggtagcggtagcggcagcaccggcgtgagcaaaggcgaagaactgtttaccggcgtggtgccgattctggtggaactggatggtgatgtgaacggccataaatttagcgtgagcggcgaaggcgaaggtgatgcgacctatggcaaactgaccctgaaattcatttgcaccaccggcaaactgccggttccgtggccgaccctggttaccaccctgacctggggcgtgcagtgctttgcgcgttatccggatcatatgaaacagcacgatttctttaaaagcgccatgccggaaggctatgtgcaggaacgcaccatcttttttaaagatgatggcaactataaaacccgtgcggaagtgaaatttgaaggcgataccctggtgaaccgtattgaactgaaaggcatcgatttcaaagaagatggcaacattctgggccataaactggaatataactacatcagcgataacgtgtatatcaccgcggataaacagaaaaacggcatcaaagcgaactttaaaatccgccacaacattgaagatggcagcgtgcagctggccgatcattatcagcagaacaccccgattggtgatggcccggtgctgctgccggataaccattatctgagcacccagagcaaactgagcaaagatccgaacgaaaaacgtgatcacatggtgctgctggaatttgtgaccgcagccggtattaccctgggcatggatgaactgtacaaaaccggcctggaagtgctgtttcagggcccgaccggccatcatcatcatcatcataccggctagtgactgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0105_sequence/1
type: Sequence
persistent identity: BBa B0105 sequence
display id: BBa_B0105_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: accggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18912_sequence/1
type: Sequence
persistent identity: BBa J18912 sequence
display id: BBa_J18912_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagaccacaacggtttccctctagcaataattttgtttaactttaagaaggagatatacatatg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18919_sequence/1
type: Sequence
persistent identity: BBa J18919 sequence
display id: BBa_J18919_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctggaagtgctgtttcagggcccg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18922_sequence/1
type: Sequence
persistent identity: BBa J18922 sequence
display id: BBa_J18922_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtagcggcagcggtagcggtagcggcagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18926_sequence/1
type: Sequence
persistent identity: BBa J18926 sequence
display id: BBa_J18926_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atcctgtggcatgaaatgtggcatgaaggcctggaagaagcgagccgtctgtattttggcgaacgtaacgtgaaaggcatgtttgaagtgctggaaccgctgcatgccatgatggaacgtggcccgcagaccctgaaagaaaccagctttaaccaggcgtatggccgtgatctgatggaagcgcaggaatggtgccgtaaatacatgaaaagcggcaacgtgaaagatctgctgcaagcgtgggatctgtattatcatgtgtttcgccgcatcagcaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18930_sequence/1
type: Sequence
persistent identity: BBa J18930 sequence
display id: BBa_J18930_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtgagcaaaggcgaagaactgtttaccggcgtggtgccgattctggtggaactggatggtgatgtgaacggccataaatttagcgtgagcggcgaaggcgaaggtgatgcgacctatggcaaactgaccctgaaattcatttgcaccaccggcaaactgccggttccgtggccgaccctggttaccaccctgacctggggcgtgcagtgctttgcgcgttatccggatcatatgaaacagcacgatttctttaaaagcgccatgccggaaggctatgtgcaggaacgcaccatcttttttaaagatgatggcaactataaaacccgtgcggaagtgaaatttgaaggcgataccctggtgaaccgtattgaactgaaaggcatcgatttcaaagaagatggcaacattctgggccataaactggaatataactacatcagcgataacgtgtatatcaccgcggataaacagaaaaacggcatcaaagcgaactttaaaatccgccacaacattgaagatggcagcgtgcagctggccgatcattatcagcagaacaccccgattggtgatggcccggtgctgctgccggataaccattatctgagcacccagagcaaactgagcaaagatccgaacgaaaaacgtgatcacatggtgctgctggaatttgtgaccgcagccggtattaccctgggcatggatgaactgtacaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18909_sequence/1
type: Sequence
persistent identity: BBa J18909 sequence
display id: BBa_J18909_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: catcatcatcatcatcat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18913_sequence/1
type: Sequence
persistent identity: BBa J18913 sequence
display id: BBa_J18913_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tagtgactgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggat
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z