BBa_J18957
type: ComponentDefinition
persistent identity: BBa J18957
display id: BBa_J18957
version: 1
was generated by: iGEM to SBOL conversion
description: mCerulean ~ Lov2
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: synthetic
owned by: james
owned by: myers
mutable description: P25 expression cassette from Gruenberg et al. 2010 series of interaction reporter proteins. All proteins share the same architecture: * T7Start-domain1-5xGS-domain2-preSCsite-HisTag-T7Stop Only Domains1 and 2 vary and are taken from a list of protein interaction input and output (readout) parts. ===Expression=== Proteins were expressed in E. coli BL21 using T7 Polymerase and were purified by His-tag affinity. ===Reference=== * Gruenberg, Ferrar, van der Sloot, Serrano (2010): Building Blocks for Protein Interaction Devices
discontinued: false
dominant: false
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructs were assembled according to R.F.C. 25. All parts were codon optimized for E. coli.
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
component: BBa_B0105
component: BBa_J18912
component: BBa_J18930
component: BBa_J18919
component: BBa_J18909
component: BBa_J18922
component: BBa_J18913
component: BBa_J18927
sequence annotation: BBa_J18912
sequence annotation: BBa_B0105
sequence annotation: BBa_J18930
sequence annotation: BBa_J18922
sequence annotation: BBa_J18927
sequence annotation: BBa_J18919
sequence annotation: BBa_J18909
sequence annotation: BBa_J18913
sequence: https://synbiohub.org/public/igem/BBa_J18957_sequence/1

BBa_B0105
type: Component
persistent identity: component2066916
display id: component2066916
version: 1
top level: BBa_J18957
definition: Scar 25
access: public

BBa_J18912
type: Component
persistent identity: component2066914
display id: component2066914
version: 1
top level: BBa_J18957
definition: T7start
access: public

BBa_B0105
type: Component
persistent identity: component2066919
display id: component2066919
version: 1
top level: BBa_J18957
definition: Scar 25
access: public

BBa_J18930
type: Component
persistent identity: component2066917
display id: component2066917
version: 1
top level: BBa_J18957
definition: mCerulean
access: public

BBa_B0105
type: Component
persistent identity: component2066928
display id: component2066928
version: 1
top level: BBa_J18957
definition: Scar 25
access: public

BBa_J18919
type: Component
persistent identity: component2066926
display id: component2066926
version: 1
top level: BBa_J18957
definition: preSCsite
access: public

BBa_B0105
type: Component
persistent identity: component2066932
display id: component2066932
version: 1
top level: BBa_J18957
definition: Scar 25
access: public

BBa_J18909
type: Component
persistent identity: component2066930
display id: component2066930
version: 1
top level: BBa_J18957
definition: HisTag
access: public

BBa_B0105
type: Component
persistent identity: component2066922
display id: component2066922
version: 1
top level: BBa_J18957
definition: Scar 25
access: public

BBa_J18922
type: Component
persistent identity: component2066920
display id: component2066920
version: 1
top level: BBa_J18957
definition: 5xGS
access: public

BBa_B0105
type: Component
persistent identity: component2066925
display id: component2066925
version: 1
top level: BBa_J18957
definition: Scar 25
access: public

BBa_J18913
type: Component
persistent identity: component2066934
display id: component2066934
version: 1
top level: BBa_J18957
definition: T7stop
access: public

BBa_J18927
type: Component
persistent identity: component2066923
display id: component2066923
version: 1
top level: BBa_J18957
definition: Lov2
access: public

BBa_J18912
type: SequenceAnnotation
persistent identity: annotation2066914
display id: annotation2066914
version: 1
top level: BBa_J18957
location: https://synbiohub.org/public/igem/BBa_J18957/annotation2066914/range2066914/1
component: BBa_J18912

https://synbiohub.org/public/igem/BBa_J18957/annotation2066914/range2066914/1
type: Range
persistent identity: range2066914
display id: range2066914
version: 1
top level: BBa_J18957
direction: direction/reverse
start: 1
end: 83
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066916
display id: annotation2066916
version: 1
top level: BBa_J18957
location: https://synbiohub.org/public/igem/BBa_J18957/annotation2066916/range2066916/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18957/annotation2066916/range2066916/1
type: Range
persistent identity: range2066916
display id: range2066916
version: 1
top level: BBa_J18957
direction: direction/reverse
start: 84
end: 89
orientation: inline

BBa_J18930
type: SequenceAnnotation
persistent identity: annotation2066917
display id: annotation2066917
version: 1
top level: BBa_J18957
location: https://synbiohub.org/public/igem/BBa_J18957/annotation2066917/range2066917/1
component: BBa_J18930

https://synbiohub.org/public/igem/BBa_J18957/annotation2066917/range2066917/1
type: Range
persistent identity: range2066917
display id: range2066917
version: 1
top level: BBa_J18957
direction: direction/reverse
start: 90
end: 803
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066919
display id: annotation2066919
version: 1
top level: BBa_J18957
location: https://synbiohub.org/public/igem/BBa_J18957/annotation2066919/range2066919/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18957/annotation2066919/range2066919/1
type: Range
persistent identity: range2066919
display id: range2066919
version: 1
top level: BBa_J18957
direction: direction/reverse
start: 804
end: 809
orientation: inline

BBa_J18922
type: SequenceAnnotation
persistent identity: annotation2066920
display id: annotation2066920
version: 1
top level: BBa_J18957
location: https://synbiohub.org/public/igem/BBa_J18957/annotation2066920/range2066920/1
component: BBa_J18922

https://synbiohub.org/public/igem/BBa_J18957/annotation2066920/range2066920/1
type: Range
persistent identity: range2066920
display id: range2066920
version: 1
top level: BBa_J18957
direction: direction/reverse
start: 810
end: 839
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066922
display id: annotation2066922
version: 1
top level: BBa_J18957
location: https://synbiohub.org/public/igem/BBa_J18957/annotation2066922/range2066922/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18957/annotation2066922/range2066922/1
type: Range
persistent identity: range2066922
display id: range2066922
version: 1
top level: BBa_J18957
direction: direction/reverse
start: 840
end: 845
orientation: inline

BBa_J18927
type: SequenceAnnotation
persistent identity: annotation2066923
display id: annotation2066923
version: 1
top level: BBa_J18957
location: https://synbiohub.org/public/igem/BBa_J18957/annotation2066923/range2066923/1
component: BBa_J18927

https://synbiohub.org/public/igem/BBa_J18957/annotation2066923/range2066923/1
type: Range
persistent identity: range2066923
display id: range2066923
version: 1
top level: BBa_J18957
direction: direction/reverse
start: 846
end: 1259
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066925
display id: annotation2066925
version: 1
top level: BBa_J18957
location: https://synbiohub.org/public/igem/BBa_J18957/annotation2066925/range2066925/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18957/annotation2066925/range2066925/1
type: Range
persistent identity: range2066925
display id: range2066925
version: 1
top level: BBa_J18957
direction: direction/reverse
start: 1260
end: 1265
orientation: inline

BBa_J18919
type: SequenceAnnotation
persistent identity: annotation2066926
display id: annotation2066926
version: 1
top level: BBa_J18957
location: https://synbiohub.org/public/igem/BBa_J18957/annotation2066926/range2066926/1
component: BBa_J18919

https://synbiohub.org/public/igem/BBa_J18957/annotation2066926/range2066926/1
type: Range
persistent identity: range2066926
display id: range2066926
version: 1
top level: BBa_J18957
direction: direction/reverse
start: 1266
end: 1289
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066928
display id: annotation2066928
version: 1
top level: BBa_J18957
location: https://synbiohub.org/public/igem/BBa_J18957/annotation2066928/range2066928/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18957/annotation2066928/range2066928/1
type: Range
persistent identity: range2066928
display id: range2066928
version: 1
top level: BBa_J18957
direction: direction/reverse
start: 1290
end: 1295
orientation: inline

BBa_J18909
type: SequenceAnnotation
persistent identity: annotation2066930
display id: annotation2066930
version: 1
top level: BBa_J18957
location: https://synbiohub.org/public/igem/BBa_J18957/annotation2066930/range2066930/1
component: BBa_J18909

https://synbiohub.org/public/igem/BBa_J18957/annotation2066930/range2066930/1
type: Range
persistent identity: range2066930
display id: range2066930
version: 1
top level: BBa_J18957
direction: direction/reverse
start: 1296
end: 1313
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066932
display id: annotation2066932
version: 1
top level: BBa_J18957
location: https://synbiohub.org/public/igem/BBa_J18957/annotation2066932/range2066932/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18957/annotation2066932/range2066932/1
type: Range
persistent identity: range2066932
display id: range2066932
version: 1
top level: BBa_J18957
direction: direction/reverse
start: 1314
end: 1319
orientation: inline

BBa_J18913
type: SequenceAnnotation
persistent identity: annotation2066934
display id: annotation2066934
version: 1
top level: BBa_J18957
location: https://synbiohub.org/public/igem/BBa_J18957/annotation2066934/range2066934/1
component: BBa_J18913

https://synbiohub.org/public/igem/BBa_J18957/annotation2066934/range2066934/1
type: Range
persistent identity: range2066934
display id: range2066934
version: 1
top level: BBa_J18957
direction: direction/reverse
start: 1320
end: 1454
orientation: inline

Scar 25
type: ComponentDefinition
persistent identity: BBa B0105
display id: BBa_B0105
version: 1
was generated by: iGEM to SBOL conversion
description: RFC 25 Scar Sequence
created: 2009-10-14T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: BBF RFC 25
owned by: james
owned by: myers
mutable description: This is the scar produced by assembly using RFC 25. If you are assembling a composite part using RFC 25, you can insert this part and specify blunt assembly to get the desired sequence.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 25
owning group id: 397
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Simple DNA sequence
star: false
creator: Randy Rettberg
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: Scar 25
sequence: https://synbiohub.org/public/igem/BBa_B0105_sequence/1

Scar 25
type: SequenceAnnotation
persistent identity: annotation2041621
display id: annotation2041621
version: 1
top level: Scar 25
location: https://synbiohub.org/public/igem/BBa_B0105/annotation2041621/range2041621/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0105/annotation2041621/range2041621/1
type: Range
persistent identity: range2041621
display id: range2041621
version: 1
top level: Scar 25
direction: direction/forward
start: 1
end: 6
orientation: inline

T7start
type: ComponentDefinition
persistent identity: BBa J18912
display id: BBa_J18912
version: 1
was generated by: iGEM to SBOL conversion
description: T7 promoter + RBS + START
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: pET3a expression vector
owned by: james
owned by: myers
mutable description: Strong T7 promoter and RBS including start codon for protein expression in E. coli or in-vitro. Part is provided in RFC 25 assembly format.
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: * sequence is taken from the commonly used pET3a expression vector * deleted XbaI site between prom. and RBS * T7 promoter sequence (1-19) equals the one in BBa_I712074 * Conservative choice of part boundaries and modifications -- the part's size could probably be reduced.
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_J18912_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2065586
display id: annotation2065586
version: 1
top level: T7start
location: https://synbiohub.org/public/igem/BBa_J18912/annotation2065586/range2065586/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_J18912/annotation2065586/range2065586/1
type: Range
persistent identity: range2065586
display id: range2065586
version: 1
top level: T7start
direction: direction/forward
start: 81
end: 83
orientation: inline

preSCsite
type: ComponentDefinition
persistent identity: BBa J18919
display id: BBa_J18919
version: 1
was generated by: iGEM to SBOL conversion
description: preScission protease cleavage site (E. coli-optimized)
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: gene synthesis
owned by: james
owned by: myers
mutable description: cleavage site for PreScission??? protease. preScission protease cleavage site (PreScission??? protease is a genetically engineered form of human rhinovirus 3C protease with a GST fusion, allowing for facile cleavage and purification of GST-tagged proteins along with protease removal after recombinant protein cleavage. Enables low-temperature cleavage of fusion proteins containing the eight-residue recognition sequence.) cleavage site: * Leu-Glu-Val-Leu-Phe-Gln↓Gly-Pro References ============== Walker et al. (1994): Efficient and rapid affinity purification of proteins using recombinant fusion proteases. PMID: 7764949
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: * gene synthesis * codon optimized for E. coli
star: false
creator: Raik Gruenberg
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_J18919_sequence/1

5xGS
type: ComponentDefinition
persistent identity: BBa J18922
display id: BBa_J18922
version: 1
was generated by: iGEM to SBOL conversion
description: 10aa [GS]x linker
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: gene synthesis
owned by: james
owned by: myers
mutable description: Highly flexible 10 amino acid linker. Translates to [GS]_5. Codon-optimize for E. coli, yeast, mammalian.
discontinued: false
dominant: true
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: * gene synthesis * codon optimized for E. coli * experimental distance distributions are described in Neuweiler et al. 2006 papers.
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J18922_sequence/1

Lov2
type: ComponentDefinition
persistent identity: BBa J18927
display id: BBa_J18927
version: 1
was generated by: iGEM to SBOL conversion
description: phot1-LOV2
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: literature gene synthesis
owned by: james
owned by: myers
mutable description: The second LOV domain of Arabidopsis phototropin 1 shows light-induced transient (30s) homo-dimerization between a ground state and an excited monomer (Nakasone et al. 2006). LOV2 is predominantly monomeric at concentrations below 100microM but turns dimeric above. Funnily, the high-conc. dimer state experiences photo-dissociation instead. Either the excitation follows a different course from the dimer starting state or the excitation event itself pushes the complex apart. (CRG-internal ID: rg0090) ===References=== * Nakasone et al. 2006 PMID: 16679373 * Freddolino, Peter L.; Dittrich, Markus; Schulten, Klaus (2006) Dynamic Switching Mechanisms in LOV1 and LOV2 Domains of Plant Phototropins PMID: 16935961
discontinued: false
dominant: false
experience: experience/None
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The fragment used by Nakasone et al. is 475K - 578G. The core domain 449E - 586R has reportedly lower stability at RT (aggregation). * gene synthesis * codon optimized for E. coli
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J18927_sequence/1

mCerulean
type: ComponentDefinition
persistent identity: BBa J18930
display id: BBa_J18930
version: 1
was generated by: iGEM to SBOL conversion
description: mCerulean CFP
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: literature gene synthesis
owned by: james
owned by: myers
mutable description: Cerulean GFP is an optimized FRET donor molecule that was rationally designed by Piston and co-workers to eliminate the excited-state heterogeneity of ECFP. In ECFP, a single-exponential fit to the fluorescence lifetime is not feasible due to multiple components, whereas Cerulean exhibits essentially homogeneous excited-state decay kinetics, rendering this protein useful for lifetime imaging... Live cell imaging experiments have demonstrated that Cerulean GFP undergoes highly efficient FRET to yellow acceptor molecules (25, 26). Additionally, Cerulean exhibits more than twice the brightness of ECFP and CyPet, emitting light at fluorescence intensities similar to Citrine (20). ===References=== * Rizzo MA, Springer GH, Granada B, Piston DW. (2004) An improved cyan fluorescent protein variant useful for FRET. PMID: 14990965
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: * amino acid sequence taken from a pAG304GAL-Cerulean-ccdB from LindQuist lab * borders trimmed to match the construct used for PDB 2Q57 (last 9 AA are not resolved in PDB) * structure starts with MSK, this construct starts with MVSK but the M has been skipped for this Biobrick * gene synthesis * codon optimized for E. coli
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J18930_sequence/1

HisTag
type: ComponentDefinition
persistent identity: BBa J18909
display id: BBa_J18909
version: 1
was generated by: iGEM to SBOL conversion
description: His tag (6xHis; E. coli - optimized)
created: 2010-01-15T12:00:00Z
modified: 2015-08-31T04:08:35Z
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
mutable description: Hexahistidine affinity tag. Binds zinc, nickel, or cobalt affinity resins. =Notes= This part is identical to BBa_I757013 (which does not seem to be available though). =References= Wikipedia article: [http://en.wikipedia.org/wiki/Polyhistidine-tag]
discontinued: false
dominant: false
experience: experience/None
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Simple repetition of the His codon most frequent in E. coli -- a more balanced codon-choice may be better.
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: PolyHis
sequence: https://synbiohub.org/public/igem/BBa_J18909_sequence/1

PolyHis
type: SequenceAnnotation
persistent identity: annotation2065463
display id: annotation2065463
version: 1
top level: HisTag
location: https://synbiohub.org/public/igem/BBa_J18909/annotation2065463/range2065463/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_J18909/annotation2065463/range2065463/1
type: Range
persistent identity: range2065463
display id: range2065463
version: 1
top level: HisTag
direction: direction/forward
start: 1
end: 18
orientation: inline

T7stop
type: ComponentDefinition
persistent identity: BBa J18913
display id: BBa_J18913
version: 1
was generated by: iGEM to SBOL conversion
description: T7 terminator (incl. STOP)
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: pET3a expression plasmid
owned by: james
owned by: myers
mutable description: T7 terminator for protein expression in E. coli or in vitro. Part is provided in R.F.C. 25 assembly format.
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: * taken from pET3a expression plasmid * added two STOP to 5' * conservative choice of part boundary, could probably be shortened
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_J18913_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2065587
display id: annotation2065587
version: 1
top level: T7stop
location: https://synbiohub.org/public/igem/BBa_J18913/annotation2065587/range2065587/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_J18913/annotation2065587/range2065587/1
type: Range
persistent identity: range2065587
display id: range2065587
version: 1
top level: T7stop
direction: direction/forward
start: 1
end: 6
orientation: inline

https://synbiohub.org/public/igem/BBa_J18957_sequence/1
type: Sequence
persistent identity: BBa J18957 sequence
display id: BBa_J18957_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagaccacaacggtttccctctagcaataattttgtttaactttaagaaggagatatacatatgaccggcgtgagcaaaggcgaagaactgtttaccggcgtggtgccgattctggtggaactggatggtgatgtgaacggccataaatttagcgtgagcggcgaaggcgaaggtgatgcgacctatggcaaactgaccctgaaattcatttgcaccaccggcaaactgccggttccgtggccgaccctggttaccaccctgacctggggcgtgcagtgctttgcgcgttatccggatcatatgaaacagcacgatttctttaaaagcgccatgccggaaggctatgtgcaggaacgcaccatcttttttaaagatgatggcaactataaaacccgtgcggaagtgaaatttgaaggcgataccctggtgaaccgtattgaactgaaaggcatcgatttcaaagaagatggcaacattctgggccataaactggaatataactacatcagcgataacgtgtatatcaccgcggataaacagaaaaacggcatcaaagcgaactttaaaatccgccacaacattgaagatggcagcgtgcagctggccgatcattatcagcagaacaccccgattggtgatggcccggtgctgctgccggataaccattatctgagcacccagagcaaactgagcaaagatccgaacgaaaaacgtgatcacatggtgctgctggaatttgtgaccgcagccggtattaccctgggcatggatgaactgtacaaaaccggcggtagcggcagcggtagcggtagcggcagcaccggcgaaagcgtggatgataaagtgcgtcagaaagaaatgcgtaaaggcattgatctggccaccaccctggaacgtatcgaaaaaaacttcgtgattaccgatccgcgtctgccggataatccgattatttttgcgagcgatagctttctggaactgaccgaatatagccgtgaagaaattctgggccgtaactgccgttttctgcaaggcccggaaaccgatctgaccaccgtgaaaaaaattcgcaacgcgattgataaccagaccgaagtgaccgtgcagctgattaactataccaaaagcggcaaaaaattctggaacatctttcatctgcaaccgatgcgtgatcagaaaggcgaagtgcagtattttattggcgtgcagctggatggcagcaaacatgtggagccggtgcgtaccggcctggaagtgctgtttcagggcccgaccggccatcatcatcatcatcataccggctagtgactgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0105_sequence/1
type: Sequence
persistent identity: BBa B0105 sequence
display id: BBa_B0105_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: accggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18912_sequence/1
type: Sequence
persistent identity: BBa J18912 sequence
display id: BBa_J18912_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagaccacaacggtttccctctagcaataattttgtttaactttaagaaggagatatacatatg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18919_sequence/1
type: Sequence
persistent identity: BBa J18919 sequence
display id: BBa_J18919_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctggaagtgctgtttcagggcccg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18922_sequence/1
type: Sequence
persistent identity: BBa J18922 sequence
display id: BBa_J18922_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtagcggcagcggtagcggtagcggcagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18927_sequence/1
type: Sequence
persistent identity: BBa J18927 sequence
display id: BBa_J18927_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaaagcgtggatgataaagtgcgtcagaaagaaatgcgtaaaggcattgatctggccaccaccctggaacgtatcgaaaaaaacttcgtgattaccgatccgcgtctgccggataatccgattatttttgcgagcgatagctttctggaactgaccgaatatagccgtgaagaaattctgggccgtaactgccgttttctgcaaggcccggaaaccgatctgaccaccgtgaaaaaaattcgcaacgcgattgataaccagaccgaagtgaccgtgcagctgattaactataccaaaagcggcaaaaaattctggaacatctttcatctgcaaccgatgcgtgatcagaaaggcgaagtgcagtattttattggcgtgcagctggatggcagcaaacatgtggagccggtgcgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18930_sequence/1
type: Sequence
persistent identity: BBa J18930 sequence
display id: BBa_J18930_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtgagcaaaggcgaagaactgtttaccggcgtggtgccgattctggtggaactggatggtgatgtgaacggccataaatttagcgtgagcggcgaaggcgaaggtgatgcgacctatggcaaactgaccctgaaattcatttgcaccaccggcaaactgccggttccgtggccgaccctggttaccaccctgacctggggcgtgcagtgctttgcgcgttatccggatcatatgaaacagcacgatttctttaaaagcgccatgccggaaggctatgtgcaggaacgcaccatcttttttaaagatgatggcaactataaaacccgtgcggaagtgaaatttgaaggcgataccctggtgaaccgtattgaactgaaaggcatcgatttcaaagaagatggcaacattctgggccataaactggaatataactacatcagcgataacgtgtatatcaccgcggataaacagaaaaacggcatcaaagcgaactttaaaatccgccacaacattgaagatggcagcgtgcagctggccgatcattatcagcagaacaccccgattggtgatggcccggtgctgctgccggataaccattatctgagcacccagagcaaactgagcaaagatccgaacgaaaaacgtgatcacatggtgctgctggaatttgtgaccgcagccggtattaccctgggcatggatgaactgtacaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18909_sequence/1
type: Sequence
persistent identity: BBa J18909 sequence
display id: BBa_J18909_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: catcatcatcatcatcat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18913_sequence/1
type: Sequence
persistent identity: BBa J18913 sequence
display id: BBa_J18913_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tagtgactgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggat
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z