BBa_J202001 1 BBa_J202001 PBAD Mutant#2 2012-07-18T11:00:00Z 2015-08-31T04:08:36Z This mutant was created by random mutagenesis. This is PBAD (BBa_I13453) mutant. -95 relative to the araC transcriptional start site was changed from C to T. Also, T at -83 was changed to A. false false _910_ 0 11164 9 It's complicated false In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose. false Jiyeon Park annotation2177776 1 Transcription Start range2177776 1 112 112 annotation2177778 1 Mutation 2 range2177778 1 29 29 annotation2177775 1 araI2 range2177775 1 61 77 annotation2177774 1 araI1 range2177774 1 40 56 annotation2177777 1 Mutation 1 range2177777 1 17 17 BBa_J202001_sequence 1 acattgattatttgcatggcgtcacactatgctatgccatagcatttttatccataggattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z