LOCUS       BBa_J202025             1014 bp    DNA     linear       27-Dec-2024
DEFINITION  PBAD Characterization (Mutant#10)
ACCESSION   BBa_J202025
VERSION     BBa_J202025.1  
FEATURES             Location/Qualifiers
     misc_feature    53..53
                     /label=Mutation 1
     misc_feature    64..64
                     /label=Mutation 2
     misc_feature    112..112
                     /label=Transcription Start
     misc_feature    40..56
                     /label=araI1
     misc_feature    61..77
                     /label=araI2
     promoter        1..130
                     /label=BBa_J202009
     RBS             139..151
                     /label=RBS-3\Weak
     RBS             145..148
                     /label=RBS
     misc_feature    139..151
                     /label=BBa_B0032
     RBS             139..151
                     /label=BBa_B0032
     CDS             158..877
                     /label=GFP protein
     CDS             158..877
                     /label=BBa_E0040
     stem_loop       897..940
                     /label=stem_loop
     misc_feature    886..965
                     /label=BBa_B0010
     terminator      886..965
                     /label=BBa_B0010
     stem_loop       981..1000
                     /label=T7 TE
     misc_feature    1007..1007
                     /label=stop
     polyA_site      1001..1014
                     /label=polya
     misc_feature    974..1014
                     /label=BBa_B0012
     terminator      974..1014
                     /label=BBa_B0012
     misc_feature    139..1014
                     /label=BBa_E0240
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tctataagat
       61 tagtggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagtc acacaggaaa gtactagatg cgtaaaggag aagaactttt
      181 cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc
      241 tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg
      301 cactactgga aaactacctg ttccatggcc aacacttgtc actactttcg gttatggtgt
      361 tcaatgcttt gcgagatacc cagatcatat gaaacagcat gactttttca agagtgccat
      421 gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac
      481 acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat
      541 tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca
      601 caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag
      661 acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat
      721 tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc
      781 gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg
      841 gattacacat ggcatggatg aactatacaa ataataatac tagagccagg catcaaataa
      901 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
      961 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//