LOCUS BBa_J21000 2916 bp DNA linear 22-Dec-2024 DEFINITION LuxR mCherry, TetR ECFP, LacI EYFP ACCESSION BBa_J21000 VERSION BBa_J21000.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 20..25 /label=-35 promoter 42..47 /label=-10 misc_feature 53..53 /label=start misc_feature 1..55 /label=BBa_R0062 promoter 1..55 /label=BBa_R0062 RBS 64..78 /label=RBS-1\Strong RBS 71..75 /label=RBS misc_feature 64..78 /label=BBa_B0030 RBS 64..78 /label=BBa_B0030 CDS 85..804 /label=GFP protein CDS 85..804 /label=BBa_E0040 stem_loop 824..867 /label=stem_loop misc_feature 813..892 /label=BBa_B0010 terminator 813..892 /label=BBa_B0010 stem_loop 908..927 /label=T7 TE misc_feature 934..934 /label=stop polyA_site 928..941 /label=polya misc_feature 901..941 /label=BBa_B0012 terminator 901..941 /label=BBa_B0012 misc_feature 952..968 /label=lac O1 promoter 969..974 /label=-35 misc_feature 975..991 /label=lac O1 promoter 992..997 /label=-10 misc_feature 950..1003 /label=BBa_R0011 promoter 950..1003 /label=BBa_R0011 misc_feature 1017..1020 /label=conserved RBS 1013..1024 /label=BBa_B0034 CDS 1031..1792 /label=YFP (LVA) misc_feature 1787..1792 /label=2 misc_feature 1748..1786 /label=SsrA misc_feature 1272..1272 /label=A (Q->L) misc_feature 1031..1792 /label=BBa_E0032 misc_feature 1031..1792 /label=BBa_E0032 stem_loop 1812..1855 /label=stem_loop misc_feature 1801..1880 /label=BBa_B0010 terminator 1801..1880 /label=BBa_B0010 stem_loop 1896..1915 /label=T7 TE misc_feature 1922..1922 /label=stop polyA_site 1916..1929 /label=polya misc_feature 1889..1929 /label=BBa_B0012 terminator 1889..1929 /label=BBa_B0012 misc_feature 1938..1956 /label=TetR 1 misc_feature 1938..1991 /label=BBa_R0040 promoter 1957..1962 /label=-35 misc_feature 1963..1981 /label=TetR 2 promoter 1980..1985 /label=-10 promoter 1938..1991 /label=BBa_R0040 misc_feature 2004..2007 /label=conserved RBS 2000..2011 /label=BBa_B0034 CDS 2018..2779 /label=CFP (LVA) misc_feature 2736..2773 /label=SsrA misc_feature 2774..2779 /label=2 misc_feature 2086..2086 /label=A misc_feature 2018..2779 /label=BBa_E0022 misc_feature 2018..2779 /label=BBa_E0022 stem_loop 2799..2842 /label=stem_loop misc_feature 2788..2867 /label=BBa_B0010 terminator 2788..2867 /label=BBa_B0010 stem_loop 2883..2902 /label=T7 TE misc_feature 2909..2909 /label=stop polyA_site 2903..2916 /label=polya misc_feature 2876..2916 /label=BBa_B0012 terminator 2876..2916 /label=BBa_B0012 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta 61 gagattaaag aggagaaata ctagatgcgt aaaggagaag aacttttcac tggagttgtc 121 ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag 181 ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa 241 ctacctgttc catggccaac acttgtcact actttcggtt atggtgttca atgctttgcg 301 agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat 361 gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc 421 aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa 481 gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc 541 atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa 601 gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct 661 gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac 721 gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc 781 atggatgaac tatacaaata ataatactag agccaggcat caaataaaac gaaaggctca 841 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 901 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga attgtgagcg 961 gataacaatt gacattgtga gcggataaca agatactgag cacatactag agaaagagga 1021 gaaatactag atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt 1081 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga 1141 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc 1201 ctggcccacc ctcgtgacca ccttcggcta cggcctgcaa tgcttcgccc gctaccccga 1261 ccacatgaag ctgcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg 1321 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg 1381 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat 1441 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa 1501 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt 1561 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc 1621 cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga 1681 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct 1741 gtacaagagg cctgctgcaa acgacgaaaa ctacgcttta gtagcttaat aatactagag 1801 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 1861 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 1921 gcgtttatat actagagtcc ctatcagtga tagagattga catccctatc agtgatagag 1981 atactgagca ctactagaga aagaggagaa atactagatg gtgagcaagg gcgaggagct 2041 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtgaacg gccacaagtt 2101 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat 2161 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg 2221 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc 2281 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa 2341 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg 2401 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacatcag 2461 ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat 2521 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc 2581 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct 2641 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc 2701 cgggatcact ctcggcatgg acgagctgta caagaggcct gctgcaaacg acgaaaacta 2761 cgctttagta gcttaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga 2821 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 2881 ctggctcacc ttcgggtggg cctttctgcg tttata //