LOCUS       BBa_J21003               904 bp    DNA     linear       13-May-2024
DEFINITION  Promoter + TetR
ACCESSION   BBa_J21003
VERSION     BBa_J21003.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=OR3
     misc_feature    11..28
                     /label=OR2
     misc_feature    34..51
                     /label=OR1
     promoter        18..23
                     /label=-35
     promoter        8..13
                     /label=-35
     promoter        30..35
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0053
     promoter        1..54
                     /label=BBa_R0053
     misc_feature    69..72
                     /label=conserved
     RBS             63..76
                     /label=BBa_B0031
     CDS             86..702
                     /label=tetR
     misc_feature    703..736
                     /label=SsrA
     misc_feature    743..767
                     /label=Help:Barcodes
     CDS             83..742
                     /label=BBa_C0040
     stem_loop       787..830
                     /label=stem_loop
     misc_feature    776..855
                     /label=BBa_B0010
     terminator      776..855
                     /label=BBa_B0010
     stem_loop       871..890
                     /label=T7 TE
     misc_feature    897..897
                     /label=stop
     polyA_site      891..904
                     /label=polya
     misc_feature    864..904
                     /label=BBa_B0012
     terminator      864..904
                     /label=BBa_B0012
     misc_feature    63..904
                     /label=BBa_P0140
ORIGIN
        1 aataaacttg actaaagatt cctttagtag ataatttaag tgttctttaa tttctactag
       61 agtcacacag gaaacctact agatgtccag attagataaa agtaaagtga ttaacagcgc
      121 attagagctg cttaatgagg tcggaatcga aggtttaaca acccgtaaac tcgcccagaa
      181 gctaggtgta gagcagccta cattgtattg gcatgtaaaa aataagcggg ctttgctcga
      241 cgccttagcc attgagatgt tagataggca ccatactcac ttttgccctt tagaagggga
      301 aagctggcaa gattttttac gtaataacgc taaaagtttt agatgtgctt tactaagtca
      361 tcgcgatgga gcaaaagtac atttaggtac acggcctaca gaaaaacagt atgaaactct
      421 cgaaaatcaa ttagcctttt tatgccaaca aggtttttca ctagagaatg cattatatgc
      481 actcagcgct gtggggcatt ttactttagg ttgcgtattg gaagatcaag agcatcaagt
      541 cgctaaagaa gaaagggaaa cacctactac tgatagtatg ccgccattat tacgacaagc
      601 tatcgaatta tttgatcacc aaggtgcaga gccagccttc ttattcggcc ttgaattgat
      661 catatgcgga ttagaaaaac aacttaaatg tgaaagtggg tccgctgcaa acgacgaaaa
      721 ctacgcttta gtagcttaat aacactgata gtgctagtgt agatcactac tagagccagg
      781 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
      841 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
      901 tata
//