LOCUS       BBa_J21004              1372 bp    DNA     linear       25-Nov-2024
DEFINITION  Promoter + LacL
ACCESSION   BBa_J21004
VERSION     BBa_J21004.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=OR3
     misc_feature    11..28
                     /label=OR2
     misc_feature    34..51
                     /label=OR1
     promoter        18..23
                     /label=-35
     promoter        8..13
                     /label=-35
     promoter        30..35
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0053
     promoter        1..54
                     /label=BBa_R0053
     misc_feature    69..72
                     /label=conserved
     RBS             63..76
                     /label=BBa_B0031
     misc_feature    1172..1210
                     /label=LVA
     CDS             83..1210
                     /label=lacI-LVA
     misc_feature    83..1210
                     /label=BBa_C0012
     misc_feature    1211..1235
                     /label=Help:Barcodes
     CDS             83..1210
                     /label=BBa_C0012
     stem_loop       1255..1298
                     /label=stem_loop
     misc_feature    1244..1323
                     /label=BBa_B0010
     terminator      1244..1323
                     /label=BBa_B0010
     stem_loop       1339..1358
                     /label=T7 TE
     misc_feature    1365..1365
                     /label=stop
     polyA_site      1359..1372
                     /label=polya
     misc_feature    1332..1372
                     /label=BBa_B0012
     terminator      1332..1372
                     /label=BBa_B0012
     misc_feature    63..1372
                     /label=BBa_P0112
ORIGIN
        1 aataaacttg actaaagatt cctttagtag ataatttaag tgttctttaa tttctactag
       61 agtcacacag gaaacctact agatggtgaa tgtgaaacca gtaacgttat acgatgtcgc
      121 agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt
      181 ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa
      241 ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag
      301 tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact
      361 gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc
      421 ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga
      481 ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt
      541 ctctgaccag acacccatca acagtattat tttctcccat gaagacggta cgcgactggg
      601 cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag
      661 ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat
      721 tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat
      781 gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc
      841 gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt
      901 agtgggatac gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa
      961 acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg
     1021 ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct
     1081 ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     1141 acgacaggtt tcccgactgg aaagcgggca ggctgcaaac gacgaaaact acgctttagt
     1201 agcttaataa ctctgatagt gctagtgtag atctctacta gagccaggca tcaaataaaa
     1261 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1321 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
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