##gff-version 3
##sequence-region BBa_J21004 1 1372
BBa_J21004	.	engineered_region	63	1372	.	+	0	ID=BBa_P0112;Name=BBa_P0112
BBa_J21004	.	ribosome_entry_site	63	76	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_P0112
BBa_J21004	.	conserved_region	69	72	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_J21004	.	terminator	1244	1323	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0112
BBa_J21004	.	engineered_region	1244	1323	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J21004	.	stem_loop	1255	1298	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J21004	.	CDS	83	1210	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_P0112
BBa_J21004	.	engineered_tag	1211	1235	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_J21004	.	CDS	83	1210	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_J21004	.	tag	1172	1210	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_J21004	.	engineered_region	83	1210	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_J21004	.	terminator	1332	1372	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0112
BBa_J21004	.	engineered_region	1332	1372	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J21004	.	stem_loop	1339	1358	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J21004	.	stop_codon	1365	1365	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J21004	.	polyA_site	1359	1372	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J21004	.	promoter	1	54	.	+	0	ID=BBa_R0053;Name=BBa_R0053
BBa_J21004	.	promoter	18	23	.	+	0	ID=annotation2038;Name=-35;Parent=BBa_R0053
BBa_J21004	.	promoter	8	13	.	+	0	ID=annotation2041;Name=-35;Parent=BBa_R0053
BBa_J21004	.	non_covalent_binding_site	1	3	.	+	0	ID=annotation2035;Name=OR3;Parent=BBa_R0053
BBa_J21004	.	engineered_region	1	54	.	+	0	ID=annotation7069;Name=BBa_R0053;Parent=BBa_R0053
BBa_J21004	.	promoter	30	35	.	+	0	ID=annotation2042;Name=-10;Parent=BBa_R0053
BBa_J21004	.	non_covalent_binding_site	34	51	.	+	0	ID=annotation2037;Name=OR1;Parent=BBa_R0053
BBa_J21004	.	non_covalent_binding_site	11	28	.	+	0	ID=annotation2036;Name=OR2;Parent=BBa_R0053
>BBa_J21004
aataaacttgactaaagattcctttagtagataatttaagtgttctttaatttctactagagtcacacaggaaacctac
tagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcg
tggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcc
caaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcg
ccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaa
gcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgct
ggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagaca
cccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagc
aaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcg
caatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctg
aatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagt
ccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgtt
aaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcg
gtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctc
cccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacga
aaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttata