##gff-version 3
##sequence-region BBa_J21005 1 2829
BBa_J21005	.	terminator	2701	2780	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J21005	.	stem_loop	2712	2755	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J21005	.	engineered_region	2701	2780	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J21005	.	terminator	804	883	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J21005	.	stem_loop	815	858	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J21005	.	engineered_region	804	883	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J21005	.	CDS	1022	1744	.	+	0	ID=BBa_E0030;Name=BBa_E0030
BBa_J21005	.	ribosome_entry_site	1952	1963	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J21005	.	conserved_region	1956	1959	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J21005	.	promoter	1890	1943	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J21005	.	engineered_region	1890	1943	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J21005	.	non_covalent_binding_site	1890	1908	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J21005	.	non_covalent_binding_site	1915	1933	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J21005	.	promoter	1909	1914	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J21005	.	promoter	1932	1937	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J21005	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J21005	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J21005	.	terminator	1753	1832	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J21005	.	stem_loop	1764	1807	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J21005	.	engineered_region	1753	1832	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J21005	.	ribosome_entry_site	1004	1015	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J21005	.	conserved_region	1008	1011	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J21005	.	promoter	1	55	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_J21005	.	engineered_region	1	55	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J21005	.	promoter	20	25	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J21005	.	promoter	42	47	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J21005	.	start_codon	53	53	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J21005	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J21005	.	engineered_region	82	795	.	+	0	ID=BBa_J06504;Name=BBa_J06504
BBa_J21005	.	sequence_alteration	433	433	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_J21005	.	CDS	82	792	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_J21005	.	promoter	941	994	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_J21005	.	promoter	960	965	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J21005	.	promoter	983	988	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J21005	.	engineered_region	941	994	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J21005	.	operator	966	982	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J21005	.	operator	943	959	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J21005	.	terminator	1841	1881	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J21005	.	polyA_site	1868	1881	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J21005	.	engineered_region	1841	1881	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J21005	.	stem_loop	1848	1867	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J21005	.	stop_codon	1874	1874	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J21005	.	terminator	2789	2829	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J21005	.	polyA_site	2816	2829	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J21005	.	engineered_region	2789	2829	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J21005	.	stem_loop	2796	2815	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J21005	.	stop_codon	2822	2822	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J21005	.	terminator	892	932	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J21005	.	polyA_site	919	932	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J21005	.	engineered_region	892	932	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J21005	.	stem_loop	899	918	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J21005	.	stop_codon	925	925	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J21005	.	CDS	1970	2692	.	+	0	ID=BBa_E0020;Name=BBa_E0020
>BBa_J21005
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatact
agatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctc
cgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaag
gtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtga
agcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcga
ggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggc
accaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccg
aggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagac
cacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaac
gaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagt
aataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgt
cggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtga
gcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatggtg
agcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttca
gcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcc
cgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctg
cacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactaca
agacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggagga
cggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaac
ggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaaca
cccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaa
cgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaag
taataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttg
tcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctat
cagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggtg
agcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttca
gcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcc
cgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcag
cacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactaca
agacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggagga
cggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaac
ggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaaca
cccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaa
cgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaag
taataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttg
tcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata