LOCUS       BBa_J21006              1910 bp    DNA     linear       22-Dec-2024
DEFINITION  LuxR, TetR Generator
ACCESSION   BBa_J21006
VERSION     BBa_J21006.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=OR3
     misc_feature    11..28
                     /label=OR2
     misc_feature    34..51
                     /label=OR1
     promoter        18..23
                     /label=-35
     promoter        8..13
                     /label=-35
     promoter        30..35
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0053
     promoter        1..54
                     /label=BBa_R0053
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     misc_feature    63..998
                     /label=BBa_I0462
     promoter        1..998
                     /label=BBa_J21002
     misc_feature    1007..1009
                     /label=OR3
     misc_feature    1017..1034
                     /label=OR2
     misc_feature    1040..1057
                     /label=OR1
     promoter        1024..1029
                     /label=-35
     promoter        1014..1019
                     /label=-35
     promoter        1036..1041
                     /label=-10
     misc_feature    1007..1060
                     /label=BBa_R0053
     promoter        1007..1060
                     /label=BBa_R0053
     misc_feature    1075..1078
                     /label=conserved
     RBS             1069..1082
                     /label=BBa_B0031
     CDS             1092..1708
                     /label=tetR
     misc_feature    1709..1742
                     /label=SsrA
     misc_feature    1749..1773
                     /label=Help:Barcodes
     CDS             1089..1748
                     /label=BBa_C0040
     stem_loop       1793..1836
                     /label=stem_loop
     misc_feature    1782..1861
                     /label=BBa_B0010
     terminator      1782..1861
                     /label=BBa_B0010
     stem_loop       1877..1896
                     /label=T7 TE
     misc_feature    1903..1903
                     /label=stop
     polyA_site      1897..1910
                     /label=polya
     misc_feature    1870..1910
                     /label=BBa_B0012
     terminator      1870..1910
                     /label=BBa_B0012
     misc_feature    1069..1910
                     /label=BBa_P0140
     promoter        1007..1910
                     /label=BBa_J21003
ORIGIN
        1 aataaacttg actaaagatt cctttagtag ataatttaag tgttctttaa tttctactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagaata aacttgacta
     1021 aagattcctt tagtagataa tttaagtgtt ctttaatttc tactagagtc acacaggaaa
     1081 cctactagat gtccagatta gataaaagta aagtgattaa cagcgcatta gagctgctta
     1141 atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta ggtgtagagc
     1201 agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc ttagccattg
     1261 agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc tggcaagatt
     1321 ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc gatggagcaa
     1381 aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa aatcaattag
     1441 cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc agcgctgtgg
     1501 ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct aaagaagaaa
     1561 gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc gaattatttg
     1621 atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata tgcggattag
     1681 aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac gctttagtag
     1741 cttaataaca ctgatagtgc tagtgtagat cactactaga gccaggcatc aaataaaacg
     1801 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1861 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//