LOCUS BBa_J21007 3290 bp DNA linear 22-Nov-2024 DEFINITION LuxR,TetR,LacL Generator ACCESSION BBa_J21007 VERSION BBa_J21007.1 FEATURES Location/Qualifiers misc_feature 1..3 /label=OR3 misc_feature 11..28 /label=OR2 misc_feature 34..51 /label=OR1 promoter 18..23 /label=-35 promoter 8..13 /label=-35 promoter 30..35 /label=-10 misc_feature 1..54 /label=BBa_R0053 promoter 1..54 /label=BBa_R0053 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..82 /label=prefix misc_feature 254..254 /label=T misc_feature 572..572 /label=A CDS 81..830 /label=luxR misc_feature 81..836 /label=BBa_C0062 misc_feature 837..861 /label=Help:Barcodes CDS 81..836 /label=BBa_C0062 stem_loop 881..924 /label=stem_loop misc_feature 870..949 /label=BBa_B0010 terminator 870..949 /label=BBa_B0010 stem_loop 965..984 /label=T7 TE misc_feature 991..991 /label=stop polyA_site 985..998 /label=polya misc_feature 958..998 /label=BBa_B0012 terminator 958..998 /label=BBa_B0012 misc_feature 63..998 /label=BBa_I0462 promoter 1..998 /label=BBa_J21002 misc_feature 1007..1009 /label=OR3 misc_feature 1017..1034 /label=OR2 misc_feature 1040..1057 /label=OR1 promoter 1024..1029 /label=-35 promoter 1014..1019 /label=-35 promoter 1036..1041 /label=-10 misc_feature 1007..1060 /label=BBa_R0053 promoter 1007..1060 /label=BBa_R0053 misc_feature 1075..1078 /label=conserved RBS 1069..1082 /label=BBa_B0031 CDS 1092..1708 /label=tetR misc_feature 1709..1742 /label=SsrA misc_feature 1749..1773 /label=Help:Barcodes CDS 1089..1748 /label=BBa_C0040 stem_loop 1793..1836 /label=stem_loop misc_feature 1782..1861 /label=BBa_B0010 terminator 1782..1861 /label=BBa_B0010 stem_loop 1877..1896 /label=T7 TE misc_feature 1903..1903 /label=stop polyA_site 1897..1910 /label=polya misc_feature 1870..1910 /label=BBa_B0012 terminator 1870..1910 /label=BBa_B0012 misc_feature 1069..1910 /label=BBa_P0140 promoter 1007..1910 /label=BBa_J21003 promoter 1..1910 /label=BBa_J21006 misc_feature 1919..1921 /label=OR3 misc_feature 1929..1946 /label=OR2 misc_feature 1952..1969 /label=OR1 promoter 1936..1941 /label=-35 promoter 1926..1931 /label=-35 promoter 1948..1953 /label=-10 misc_feature 1919..1972 /label=BBa_R0053 promoter 1919..1972 /label=BBa_R0053 misc_feature 1987..1990 /label=conserved RBS 1981..1994 /label=BBa_B0031 misc_feature 3090..3128 /label=LVA CDS 2001..3128 /label=lacI-LVA misc_feature 2001..3128 /label=BBa_C0012 misc_feature 3129..3153 /label=Help:Barcodes CDS 2001..3128 /label=BBa_C0012 stem_loop 3173..3216 /label=stem_loop misc_feature 3162..3241 /label=BBa_B0010 terminator 3162..3241 /label=BBa_B0010 stem_loop 3257..3276 /label=T7 TE misc_feature 3283..3283 /label=stop polyA_site 3277..3290 /label=polya misc_feature 3250..3290 /label=BBa_B0012 terminator 3250..3290 /label=BBa_B0012 misc_feature 1981..3290 /label=BBa_P0112 promoter 1919..3290 /label=BBa_J21004 ORIGIN 1 aataaacttg actaaagatt cctttagtag ataatttaag tgttctttaa tttctactag 61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagaata aacttgacta 1021 aagattcctt tagtagataa tttaagtgtt ctttaatttc tactagagtc acacaggaaa 1081 cctactagat gtccagatta gataaaagta aagtgattaa cagcgcatta gagctgctta 1141 atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta ggtgtagagc 1201 agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc ttagccattg 1261 agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc tggcaagatt 1321 ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc gatggagcaa 1381 aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa aatcaattag 1441 cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc agcgctgtgg 1501 ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct aaagaagaaa 1561 gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc gaattatttg 1621 atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata tgcggattag 1681 aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac gctttagtag 1741 cttaataaca ctgatagtgc tagtgtagat cactactaga gccaggcatc aaataaaacg 1801 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 1861 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagaa 1921 taaacttgac taaagattcc tttagtagat aatttaagtg ttctttaatt tctactagag 1981 tcacacagga aacctactag atggtgaatg tgaaaccagt aacgttatac gatgtcgcag 2041 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt 2101 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc 2161 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc 2221 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg 2281 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg 2341 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc 2401 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct 2461 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg 2521 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt 2581 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc 2641 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc 2701 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc 2761 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag 2821 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac 2881 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc 2941 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg 3001 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac 3061 gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac gctttagtag 3121 cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc aaataaaacg 3181 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 3241 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata //