##gff-version 3
##sequence-region BBa_J21007 1 3290
BBa_J21007	.	promoter	1	1910	.	+	0	ID=BBa_J21006;Name=BBa_J21006
BBa_J21007	.	promoter	1	998	.	+	0	ID=BBa_J21002;Name=BBa_J21002;Parent=BBa_J21006
BBa_J21007	.	promoter	1	54	.	+	0	ID=BBa_R0053;Name=BBa_R0053;Parent=BBa_J21002
BBa_J21007	.	promoter	18	23	.	+	0	ID=annotation2038;Name=-35;Parent=BBa_R0053
BBa_J21007	.	promoter	8	13	.	+	0	ID=annotation2041;Name=-35;Parent=BBa_R0053
BBa_J21007	.	non_covalent_binding_site	1	3	.	+	0	ID=annotation2035;Name=OR3;Parent=BBa_R0053
BBa_J21007	.	engineered_region	1	54	.	+	0	ID=annotation7069;Name=BBa_R0053;Parent=BBa_R0053
BBa_J21007	.	promoter	30	35	.	+	0	ID=annotation2042;Name=-10;Parent=BBa_R0053
BBa_J21007	.	non_covalent_binding_site	34	51	.	+	0	ID=annotation2037;Name=OR1;Parent=BBa_R0053
BBa_J21007	.	non_covalent_binding_site	11	28	.	+	0	ID=annotation2036;Name=OR2;Parent=BBa_R0053
BBa_J21007	.	engineered_region	63	998	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_J21002
BBa_J21007	.	CDS	81	836	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_J21007	.	CDS	81	830	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_J21007	.	engineered_region	81	836	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_J21007	.	engineered_region	81	82	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_J21007	.	point_mutation	572	572	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_J21007	.	engineered_tag	837	861	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_J21007	.	point_mutation	254	254	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_J21007	.	terminator	958	998	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_J21007	.	engineered_region	958	998	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J21007	.	stem_loop	965	984	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J21007	.	stop_codon	991	991	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J21007	.	polyA_site	985	998	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J21007	.	terminator	870	949	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_J21007	.	engineered_region	870	949	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J21007	.	stem_loop	881	924	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J21007	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_J21007	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J21007	.	promoter	1007	1910	.	+	0	ID=BBa_J21003;Name=BBa_J21003;Parent=BBa_J21006
BBa_J21007	.	engineered_region	1069	1910	.	+	0	ID=BBa_P0140;Name=BBa_P0140;Parent=BBa_J21003
BBa_J21007	.	CDS	1089	1748	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0140
BBa_J21007	.	engineered_tag	1749	1773	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J21007	.	CDS	1092	1708	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J21007	.	tag	1709	1742	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J21007	.	terminator	1782	1861	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0140
BBa_J21007	.	engineered_region	1782	1861	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J21007	.	stem_loop	1793	1836	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J21007	.	ribosome_entry_site	1069	1082	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_P0140
BBa_J21007	.	conserved_region	1075	1078	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_J21007	.	terminator	1870	1910	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0140
BBa_J21007	.	engineered_region	1870	1910	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J21007	.	stem_loop	1877	1896	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J21007	.	stop_codon	1903	1903	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J21007	.	polyA_site	1897	1910	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J21007	.	promoter	1007	1060	.	+	0	ID=BBa_R0053;Name=BBa_R0053;Parent=BBa_J21003
BBa_J21007	.	promoter	1024	1029	.	+	0	ID=annotation2038;Name=-35;Parent=BBa_R0053
BBa_J21007	.	promoter	1014	1019	.	+	0	ID=annotation2041;Name=-35;Parent=BBa_R0053
BBa_J21007	.	non_covalent_binding_site	1007	1009	.	+	0	ID=annotation2035;Name=OR3;Parent=BBa_R0053
BBa_J21007	.	engineered_region	1007	1060	.	+	0	ID=annotation7069;Name=BBa_R0053;Parent=BBa_R0053
BBa_J21007	.	promoter	1036	1041	.	+	0	ID=annotation2042;Name=-10;Parent=BBa_R0053
BBa_J21007	.	non_covalent_binding_site	1040	1057	.	+	0	ID=annotation2037;Name=OR1;Parent=BBa_R0053
BBa_J21007	.	non_covalent_binding_site	1017	1034	.	+	0	ID=annotation2036;Name=OR2;Parent=BBa_R0053
BBa_J21007	.	promoter	1919	3290	.	+	0	ID=BBa_J21004;Name=BBa_J21004
BBa_J21007	.	engineered_region	1981	3290	.	+	0	ID=BBa_P0112;Name=BBa_P0112;Parent=BBa_J21004
BBa_J21007	.	ribosome_entry_site	1981	1994	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_P0112
BBa_J21007	.	conserved_region	1987	1990	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_J21007	.	terminator	3162	3241	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0112
BBa_J21007	.	engineered_region	3162	3241	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J21007	.	stem_loop	3173	3216	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J21007	.	CDS	2001	3128	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_P0112
BBa_J21007	.	engineered_tag	3129	3153	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_J21007	.	CDS	2001	3128	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_J21007	.	tag	3090	3128	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_J21007	.	engineered_region	2001	3128	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_J21007	.	terminator	3250	3290	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0112
BBa_J21007	.	engineered_region	3250	3290	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J21007	.	stem_loop	3257	3276	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J21007	.	stop_codon	3283	3283	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J21007	.	polyA_site	3277	3290	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J21007	.	promoter	1919	1972	.	+	0	ID=BBa_R0053;Name=BBa_R0053;Parent=BBa_J21004
BBa_J21007	.	promoter	1936	1941	.	+	0	ID=annotation2038;Name=-35;Parent=BBa_R0053
BBa_J21007	.	promoter	1926	1931	.	+	0	ID=annotation2041;Name=-35;Parent=BBa_R0053
BBa_J21007	.	non_covalent_binding_site	1919	1921	.	+	0	ID=annotation2035;Name=OR3;Parent=BBa_R0053
BBa_J21007	.	engineered_region	1919	1972	.	+	0	ID=annotation7069;Name=BBa_R0053;Parent=BBa_R0053
BBa_J21007	.	promoter	1948	1953	.	+	0	ID=annotation2042;Name=-10;Parent=BBa_R0053
BBa_J21007	.	non_covalent_binding_site	1952	1969	.	+	0	ID=annotation2037;Name=OR1;Parent=BBa_R0053
BBa_J21007	.	non_covalent_binding_site	1929	1946	.	+	0	ID=annotation2036;Name=OR2;Parent=BBa_R0053
>BBa_J21007
aataaacttgactaaagattcctttagtagataatttaagtgttctttaatttctactagagaaagaggagaaatacta
gatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaat
caatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtta
aatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatga
tcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaa
tctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatg
gcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacat
accattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaacc
aaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtg
agcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagc
aattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaataaacttgactaaagattc
ctttagtagataatttaagtgttctttaatttctactagagtcacacaggaaacctactagatgtccagattagataaa
agtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccaga
agctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagat
gttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagt
tttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaa
ctctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggg
gcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgat
agtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttg
aattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagt
agcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttatatactagagaataaacttgactaaagattcctttagtagataatttaagtgttctttaatttctac
tagagtcacacaggaaacctactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtc
tcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcga
tggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccac
ctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtg
gtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtg
ggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatt
tcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcat
ctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctg
gctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttt
tcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggc
gcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagaca
gctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgct
gcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcg
cccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaa
gcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata