LOCUS BBa_J21008 6127 bp DNA linear 16-Jun-2024 DEFINITION WaterColorDesign ACCESSION BBa_J21008 VERSION BBa_J21008.1 FEATURES Location/Qualifiers misc_feature 1..3 /label=OR3 misc_feature 11..28 /label=OR2 misc_feature 34..51 /label=OR1 promoter 18..23 /label=-35 promoter 8..13 /label=-35 promoter 30..35 /label=-10 misc_feature 1..54 /label=BBa_R0053 promoter 1..54 /label=BBa_R0053 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..82 /label=prefix misc_feature 254..254 /label=T misc_feature 572..572 /label=A CDS 81..830 /label=luxR misc_feature 81..836 /label=BBa_C0062 misc_feature 837..861 /label=Help:Barcodes CDS 81..836 /label=BBa_C0062 stem_loop 881..924 /label=stem_loop misc_feature 870..949 /label=BBa_B0010 terminator 870..949 /label=BBa_B0010 stem_loop 965..984 /label=T7 TE misc_feature 991..991 /label=stop polyA_site 985..998 /label=polya misc_feature 958..998 /label=BBa_B0012 terminator 958..998 /label=BBa_B0012 misc_feature 63..998 /label=BBa_I0462 promoter 1..998 /label=BBa_J21002 misc_feature 1007..1009 /label=OR3 misc_feature 1017..1034 /label=OR2 misc_feature 1040..1057 /label=OR1 promoter 1024..1029 /label=-35 promoter 1014..1019 /label=-35 promoter 1036..1041 /label=-10 misc_feature 1007..1060 /label=BBa_R0053 promoter 1007..1060 /label=BBa_R0053 misc_feature 1075..1078 /label=conserved RBS 1069..1082 /label=BBa_B0031 CDS 1092..1708 /label=tetR misc_feature 1709..1742 /label=SsrA misc_feature 1749..1773 /label=Help:Barcodes CDS 1089..1748 /label=BBa_C0040 stem_loop 1793..1836 /label=stem_loop misc_feature 1782..1861 /label=BBa_B0010 terminator 1782..1861 /label=BBa_B0010 stem_loop 1877..1896 /label=T7 TE misc_feature 1903..1903 /label=stop polyA_site 1897..1910 /label=polya misc_feature 1870..1910 /label=BBa_B0012 terminator 1870..1910 /label=BBa_B0012 misc_feature 1069..1910 /label=BBa_P0140 promoter 1007..1910 /label=BBa_J21003 promoter 1..1910 /label=BBa_J21006 misc_feature 1919..1921 /label=OR3 misc_feature 1929..1946 /label=OR2 misc_feature 1952..1969 /label=OR1 promoter 1936..1941 /label=-35 promoter 1926..1931 /label=-35 promoter 1948..1953 /label=-10 misc_feature 1919..1972 /label=BBa_R0053 promoter 1919..1972 /label=BBa_R0053 misc_feature 1987..1990 /label=conserved RBS 1981..1994 /label=BBa_B0031 misc_feature 3090..3128 /label=LVA CDS 2001..3128 /label=lacI-LVA misc_feature 2001..3128 /label=BBa_C0012 misc_feature 3129..3153 /label=Help:Barcodes CDS 2001..3128 /label=BBa_C0012 stem_loop 3173..3216 /label=stem_loop misc_feature 3162..3241 /label=BBa_B0010 terminator 3162..3241 /label=BBa_B0010 stem_loop 3257..3276 /label=T7 TE misc_feature 3283..3283 /label=stop polyA_site 3277..3290 /label=polya misc_feature 3250..3290 /label=BBa_B0012 terminator 3250..3290 /label=BBa_B0012 misc_feature 1981..3290 /label=BBa_P0112 promoter 1919..3290 /label=BBa_J21004 promoter 1..3290 /label=BBa_J21007 misc_feature 3299..3318 /label=LuxR/HSL promoter 3318..3323 /label=-35 promoter 3340..3345 /label=-10 misc_feature 3351..3351 /label=start misc_feature 3299..3353 /label=BBa_R0062 promoter 3299..3353 /label=BBa_R0062 misc_feature 3366..3369 /label=conserved RBS 3362..3373 /label=BBa_B0034 CDS 3380..4090 /label=mCherry misc_feature 3731..3731 /label=C->T (removing PstI site) misc_feature 3380..4093 /label=BBa_J06504 stem_loop 4113..4156 /label=stem_loop misc_feature 4102..4181 /label=BBa_B0010 terminator 4102..4181 /label=BBa_B0010 stem_loop 4197..4216 /label=T7 TE misc_feature 4223..4223 /label=stop polyA_site 4217..4230 /label=polya misc_feature 4190..4230 /label=BBa_B0012 terminator 4190..4230 /label=BBa_B0012 misc_feature 4241..4257 /label=lac O1 promoter 4258..4263 /label=-35 misc_feature 4264..4280 /label=lac O1 promoter 4281..4286 /label=-10 misc_feature 4239..4292 /label=BBa_R0011 promoter 4239..4292 /label=BBa_R0011 misc_feature 4306..4309 /label=conserved RBS 4302..4313 /label=BBa_B0034 CDS 4320..5042 /label=BBa_E0030 stem_loop 5062..5105 /label=stem_loop misc_feature 5051..5130 /label=BBa_B0010 terminator 5051..5130 /label=BBa_B0010 stem_loop 5146..5165 /label=T7 TE misc_feature 5172..5172 /label=stop polyA_site 5166..5179 /label=polya misc_feature 5139..5179 /label=BBa_B0012 terminator 5139..5179 /label=BBa_B0012 misc_feature 5188..5206 /label=TetR 1 misc_feature 5188..5241 /label=BBa_R0040 promoter 5207..5212 /label=-35 misc_feature 5213..5231 /label=TetR 2 promoter 5230..5235 /label=-10 promoter 5188..5241 /label=BBa_R0040 misc_feature 5254..5257 /label=conserved RBS 5250..5261 /label=BBa_B0034 CDS 5268..5990 /label=BBa_E0020 stem_loop 6010..6053 /label=stem_loop misc_feature 5999..6078 /label=BBa_B0010 terminator 5999..6078 /label=BBa_B0010 stem_loop 6094..6113 /label=T7 TE misc_feature 6120..6120 /label=stop polyA_site 6114..6127 /label=polya misc_feature 6087..6127 /label=BBa_B0012 terminator 6087..6127 /label=BBa_B0012 misc_feature 3299..6127 /label=BBa_J21005 ORIGIN 1 aataaacttg actaaagatt cctttagtag ataatttaag tgttctttaa tttctactag 61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagaata aacttgacta 1021 aagattcctt tagtagataa tttaagtgtt ctttaatttc tactagagtc acacaggaaa 1081 cctactagat gtccagatta gataaaagta aagtgattaa cagcgcatta gagctgctta 1141 atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta ggtgtagagc 1201 agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc ttagccattg 1261 agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc tggcaagatt 1321 ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc gatggagcaa 1381 aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa aatcaattag 1441 cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc agcgctgtgg 1501 ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct aaagaagaaa 1561 gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc gaattatttg 1621 atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata tgcggattag 1681 aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac gctttagtag 1741 cttaataaca ctgatagtgc tagtgtagat cactactaga gccaggcatc aaataaaacg 1801 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 1861 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagaa 1921 taaacttgac taaagattcc tttagtagat aatttaagtg ttctttaatt tctactagag 1981 tcacacagga aacctactag atggtgaatg tgaaaccagt aacgttatac gatgtcgcag 2041 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt 2101 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc 2161 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc 2221 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg 2281 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg 2341 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc 2401 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct 2461 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg 2521 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt 2581 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc 2641 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc 2701 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc 2761 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag 2821 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac 2881 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc 2941 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg 3001 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac 3061 gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac gctttagtag 3121 cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc aaataaaacg 3181 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 3241 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagac 3301 ctgtaggatc gtacaggttt acgcaagaaa atggtttgtt atagtcgaat aaatactaga 3361 gaaagaggag aaatactaga tggtgagcaa gggcgaggag gataacatgg ccatcatcaa 3421 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat 3481 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac 3541 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc 3601 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga 3661 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca 3721 ggactcctcc ttgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt 3781 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg 3841 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga 3901 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct 3961 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac 4021 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct 4081 gtacaagtaa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 4141 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 4201 caccttcggg tgggcctttc tgcgtttata tactagagaa ttgtgagcgg ataacaattg 4261 acattgtgag cggataacaa gatactgagc acatactaga gaaagaggag aaatactaga 4321 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg 4381 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg 4441 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc 4501 tcgtgaccac cttcggctac ggcctgcaat gcttcgcccg ctaccccgac cacatgaagc 4561 tgcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct 4621 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg 4681 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca 4741 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg 4801 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg 4861 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact 4921 acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc 4981 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaat 5041 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 5101 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 5161 gggcctttct gcgtttatat actagagtcc ctatcagtga tagagattga catccctatc 5221 agtgatagag atactgagca ctactagaga aagaggagaa atactagatg gtgagcaagg 5281 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtgaacg 5341 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc 5401 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc 5461 tgacctgggg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct 5521 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg 5581 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg 5641 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca 5701 actacatcag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca 5761 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc 5821 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc 5881 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg 5941 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaataa tactagagcc 6001 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 6061 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 6121 gtttata //