LOCUS       BBa_J212006             2877 bp    DNA     linear       22-Nov-2024
DEFINITION  Exo2b catalytic domain fused with the cbh1 gene
ACCESSION   BBa_J212006
VERSION     BBa_J212006.1  
FEATURES             Location/Qualifiers
     CDS             1..1542
                     /label=cbh1
     misc_feature    1543..1602
                     /label=linker
     misc_feature    1602..1608
                     /label=KR site
     CDS             1609..2856
                     /label=exo2b core
     misc_feature    2857..2874
                     /label=His tag
     misc_feature    2875..2877
                     /label=stop codon
ORIGIN
        1 atgtatcgga agttggccgt catcacggcc ttcttggcca cagctcgtgc tcagtcggcc
       61 tgcactctcc aatcggagac tcacccgcct ctgacatggc agaaatgctc gtctggtggc
      121 acgtgcactc aacagacagg ctccgtggtc atcgacgcca actggcgctg gactcacgct
      181 acgaacagca gcacgaactg ctacgatggc aacacttgga gctcgaccct atgtcctgac
      241 aacgagacct gcgcgaagaa ctgctgtctg gacggtgccg cctacgcgtc cacgtacgga
      301 gttaccacga gcggtaacag cctctccatt ggctttgtca cccagtctgc gcagaagaac
      361 gttggcgctc gcctttacct tatggcgagc gacacgacct accaggaatt caccctgctt
      421 ggcaacgagt tctctttcga tgttgatgtt tcgcagctgc cgtgcggctt gaacggagct
      481 ctctacttcg tgtccatgga cgcggatggt ggcgtgagca agtatcccac caacaccgct
      541 ggcgccaagt acggcacggg gtactgtgac agccagtgtc cccgcgatct gaagttcatc
      601 aatggccagg ccaacgttga gggctgggag ccgtcatcca acaacgcgaa cacgggcatt
      661 ggaggacacg gaagctgctg ctctgagatg gatatctggg aggccaactc catctccgag
      721 gctcttaccc cccacccttg cacgactgtc ggccaggaga tctgtgaggg tgatgggtgc
      781 ggcggaactt attccgatga cagatatggc ggcacttgcg atcccgatgg ctgcgactgg
      841 aacccatacc gcctgggcaa caccagcttc tacggccctg gctcaagctt taccctcgat
      901 accaccaaga aattgaccgt tgtcacccag ttcgagacgt cgggtgccat caaccgatac
      961 tatgtccaga atggcgtcac tttccagcag cccaacgccg agcttggtag ttactctggc
     1021 aacgagctca acgatgatta ctgcacagct gaggaggcag aattcggcgg atcctctttc
     1081 tcagacaagg gcggcctgac tcagttcaag aaggctacct ctggcggcat ggttctggtc
     1141 atgagtctgt gggatgatta ctacgccaac atgctgtggc tggactccac ctacccgaca
     1201 aacgagacct cctccacacc cggtgccgtg cgcggaagct gctccaccag ctccggtgtc
     1261 cctgctcagg tcgaatctca gtctcccaac gccaaggtca ccttctccaa catcaagttc
     1321 ggacccattg gcagcaccgg caaccctagc ggcggcaacc ctcccggcgg aaacccgcct
     1381 ggcaccacca ccacccgccg cccagccact accactggaa gctctcccgg acctactcag
     1441 tctcactacg gccagtgcgg cggtattggc tacagcggcc ccacggtctg cgccagcggc
     1501 acaacttgcc aggtcctgaa cccttactac tctcagtgcc tggaggccgc cgcccgcgag
     1561 gccgccgccc gcgaggccgc cgcccgcgag gccgccgccc gcaagcgggg atgctctagg
     1621 gatgctgata aggctacggc tacacctaca gctgagccga gtgagagtcc agatgtaaca
     1681 aaggaacccg aagaaactca ggaaccagta gaaagcaaga gcccagagcc aaaggatgat
     1741 ccaaagacca gtgaaatcaa aatacctgac ctttccatag aggcatttga tattccagat
     1801 acagatagcc aggcctttgt taacaatatg aagcttggtt ggaacctagg caataccttt
     1861 gatgcccatg actgtacctg gctttccaac aagttggatt atgagaaggg ctggtcaggg
     1921 ataaagacta ctgaagagat gatccaggca gtaaaggatg ccggctttaa cactgtacgt
     1981 ataccagtat cctggcataa ccatgtctca ggcgatgacc acaccataga ccaggcctgg
     2041 ctagacaggg tccaggaggt agtagactat gctatagaca atggccttta tgccattatc
     2101 aatatacatc acgatatggg tgaggacttt atttatccaa gtagccaata catggaccag
     2161 tcctcccatt atgtaaggcg tatttggacc cagctagctg aaagatttaa ggattatgac
     2221 gaacacctaa tatttgaatc cttaaatgag ccccggatgg taggcagtaa gtatgagtgg
     2281 tggctagatt caaatgatga gagctgcaag gatgcagtag ctagcatcaa caagcttaac
     2341 caaatatttg tcgatactgt aagggccagt ggtgctaata ataagagccg ctatcttatg
     2401 gtaccaggct acgcagcctc agtggaagga gcacttaata agggctttga gcttcccaag
     2461 gatagggtag aggatagact aatcctttcc atacatgcct atacccctta caattttgcc
     2521 ttagaggctg gaggggttag tagctttgac attaataagt ccagcagtgt cagggagata
     2581 gaccagttta tgacccagct gcacgagaaa tttgtttccc aggcagtccc agttgtaata
     2641 ggtgagtttg gtgctaggaa taaaaagaac aaccttcagg acagggtgga tttctctgca
     2701 tattacgtgg catcagccag ggccaggggc atgacctgca tatggtggga caaccatgcc
     2761 tttactggag acggtgagct ctttggtatc ctagacaggt ccaaatcaag ctggaagtac
     2821 ccagagattg tagaggctat gttaaaatac gctgaccatc atcatcatca tcattga
//