BBa_J212006 1 Tc2bh Exo2b catalytic domain fused with the cbh1 gene 2012-02-03T12:00:00Z 2015-08-31T04:08:37Z Metagenomic fosmid library of a biogas digester fed with pig manure and rice straw A novel GH5 cellulase gene exo2b was identified from a metagenomic library. The fragment encoding the Exo2b catalytic domain was then fused with the cbh1 gene from Trichoderma reesei, and the fused gene was successfully expressed in T. reesei Rut-C30 .Compared to that of the parent strain, the filter paper activity (FPA) and CMCase activity of the secreted proteins of a selected transformant A1, increased 24% and 18%, respectively. false false _921_ 0 11112 9 Not in stock false The foreign gene exo2b wasfused with the native gene cbh1 to facilitate the translocation of the foreign protein in the secretory pathway of Trichoderma reesei and avoid degradatio false Xing Yan annotation2170095 1 linker range2170095 1 1543 1602 annotation2170099 1 stop codon range2170099 1 2875 2877 annotation2170097 1 exo2b core range2170097 1 1609 2856 annotation2170096 1 KR site range2170096 1 1602 1608 annotation2170098 1 His tag range2170098 1 2857 2874 annotation2170094 1 cbh1 range2170094 1 1 1542 BBa_J212006_sequence 1 atgtatcggaagttggccgtcatcacggccttcttggccacagctcgtgctcagtcggcctgcactctccaatcggagactcacccgcctctgacatggcagaaatgctcgtctggtggcacgtgcactcaacagacaggctccgtggtcatcgacgccaactggcgctggactcacgctacgaacagcagcacgaactgctacgatggcaacacttggagctcgaccctatgtcctgacaacgagacctgcgcgaagaactgctgtctggacggtgccgcctacgcgtccacgtacggagttaccacgagcggtaacagcctctccattggctttgtcacccagtctgcgcagaagaacgttggcgctcgcctttaccttatggcgagcgacacgacctaccaggaattcaccctgcttggcaacgagttctctttcgatgttgatgtttcgcagctgccgtgcggcttgaacggagctctctacttcgtgtccatggacgcggatggtggcgtgagcaagtatcccaccaacaccgctggcgccaagtacggcacggggtactgtgacagccagtgtccccgcgatctgaagttcatcaatggccaggccaacgttgagggctgggagccgtcatccaacaacgcgaacacgggcattggaggacacggaagctgctgctctgagatggatatctgggaggccaactccatctccgaggctcttaccccccacccttgcacgactgtcggccaggagatctgtgagggtgatgggtgcggcggaacttattccgatgacagatatggcggcacttgcgatcccgatggctgcgactggaacccataccgcctgggcaacaccagcttctacggccctggctcaagctttaccctcgataccaccaagaaattgaccgttgtcacccagttcgagacgtcgggtgccatcaaccgatactatgtccagaatggcgtcactttccagcagcccaacgccgagcttggtagttactctggcaacgagctcaacgatgattactgcacagctgaggaggcagaattcggcggatcctctttctcagacaagggcggcctgactcagttcaagaaggctacctctggcggcatggttctggtcatgagtctgtgggatgattactacgccaacatgctgtggctggactccacctacccgacaaacgagacctcctccacacccggtgccgtgcgcggaagctgctccaccagctccggtgtccctgctcaggtcgaatctcagtctcccaacgccaaggtcaccttctccaacatcaagttcggacccattggcagcaccggcaaccctagcggcggcaaccctcccggcggaaacccgcctggcaccaccaccacccgccgcccagccactaccactggaagctctcccggacctactcagtctcactacggccagtgcggcggtattggctacagcggccccacggtctgcgccagcggcacaacttgccaggtcctgaacccttactactctcagtgcctggaggccgccgcccgcgaggccgccgcccgcgaggccgccgcccgcgaggccgccgcccgcaagcggggatgctctagggatgctgataaggctacggctacacctacagctgagccgagtgagagtccagatgtaacaaaggaacccgaagaaactcaggaaccagtagaaagcaagagcccagagccaaaggatgatccaaagaccagtgaaatcaaaatacctgacctttccatagaggcatttgatattccagatacagatagccaggcctttgttaacaatatgaagcttggttggaacctaggcaatacctttgatgcccatgactgtacctggctttccaacaagttggattatgagaagggctggtcagggataaagactactgaagagatgatccaggcagtaaaggatgccggctttaacactgtacgtataccagtatcctggcataaccatgtctcaggcgatgaccacaccatagaccaggcctggctagacagggtccaggaggtagtagactatgctatagacaatggcctttatgccattatcaatatacatcacgatatgggtgaggactttatttatccaagtagccaatacatggaccagtcctcccattatgtaaggcgtatttggacccagctagctgaaagatttaaggattatgacgaacacctaatatttgaatccttaaatgagccccggatggtaggcagtaagtatgagtggtggctagattcaaatgatgagagctgcaaggatgcagtagctagcatcaacaagcttaaccaaatatttgtcgatactgtaagggccagtggtgctaataataagagccgctatcttatggtaccaggctacgcagcctcagtggaaggagcacttaataagggctttgagcttcccaaggatagggtagaggatagactaatcctttccatacatgcctataccccttacaattttgccttagaggctggaggggttagtagctttgacattaataagtccagcagtgtcagggagatagaccagtttatgacccagctgcacgagaaatttgtttcccaggcagtcccagttgtaataggtgagtttggtgctaggaataaaaagaacaaccttcaggacagggtggatttctctgcatattacgtggcatcagccagggccaggggcatgacctgcatatggtgggacaaccatgcctttactggagacggtgagctctttggtatcctagacaggtccaaatcaagctggaagtacccagagattgtagaggctatgttaaaatacgctgaccatcatcatcatcatcattga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z