LOCUS BBa_J212100 2893 bp DNA linear 26-Nov-2024 DEFINITION test ACCESSION BBa_J212100 VERSION BBa_J212100.1 FEATURES Location/Qualifiers CDS 1..1542 /label=cbh1 misc_feature 1543..1602 /label=linker misc_feature 1602..1608 /label=KR site CDS 1609..2856 /label=exo2b core misc_feature 2857..2874 /label=His tag misc_feature 2875..2877 /label=stop codon CDS 1..2877 /label=BBa_J212006 RBS 2886..2890 /label=rbs misc_feature 2886..2893 /label=BBa_J212099 ORIGIN 1 atgtatcgga agttggccgt catcacggcc ttcttggcca cagctcgtgc tcagtcggcc 61 tgcactctcc aatcggagac tcacccgcct ctgacatggc agaaatgctc gtctggtggc 121 acgtgcactc aacagacagg ctccgtggtc atcgacgcca actggcgctg gactcacgct 181 acgaacagca gcacgaactg ctacgatggc aacacttgga gctcgaccct atgtcctgac 241 aacgagacct gcgcgaagaa ctgctgtctg gacggtgccg cctacgcgtc cacgtacgga 301 gttaccacga gcggtaacag cctctccatt ggctttgtca cccagtctgc gcagaagaac 361 gttggcgctc gcctttacct tatggcgagc gacacgacct accaggaatt caccctgctt 421 ggcaacgagt tctctttcga tgttgatgtt tcgcagctgc cgtgcggctt gaacggagct 481 ctctacttcg tgtccatgga cgcggatggt ggcgtgagca agtatcccac caacaccgct 541 ggcgccaagt acggcacggg gtactgtgac agccagtgtc cccgcgatct gaagttcatc 601 aatggccagg ccaacgttga gggctgggag ccgtcatcca acaacgcgaa cacgggcatt 661 ggaggacacg gaagctgctg ctctgagatg gatatctggg aggccaactc catctccgag 721 gctcttaccc cccacccttg cacgactgtc ggccaggaga tctgtgaggg tgatgggtgc 781 ggcggaactt attccgatga cagatatggc ggcacttgcg atcccgatgg ctgcgactgg 841 aacccatacc gcctgggcaa caccagcttc tacggccctg gctcaagctt taccctcgat 901 accaccaaga aattgaccgt tgtcacccag ttcgagacgt cgggtgccat caaccgatac 961 tatgtccaga atggcgtcac tttccagcag cccaacgccg agcttggtag ttactctggc 1021 aacgagctca acgatgatta ctgcacagct gaggaggcag aattcggcgg atcctctttc 1081 tcagacaagg gcggcctgac tcagttcaag aaggctacct ctggcggcat ggttctggtc 1141 atgagtctgt gggatgatta ctacgccaac atgctgtggc tggactccac ctacccgaca 1201 aacgagacct cctccacacc cggtgccgtg cgcggaagct gctccaccag ctccggtgtc 1261 cctgctcagg tcgaatctca gtctcccaac gccaaggtca ccttctccaa catcaagttc 1321 ggacccattg gcagcaccgg caaccctagc ggcggcaacc ctcccggcgg aaacccgcct 1381 ggcaccacca ccacccgccg cccagccact accactggaa gctctcccgg acctactcag 1441 tctcactacg gccagtgcgg cggtattggc tacagcggcc ccacggtctg cgccagcggc 1501 acaacttgcc aggtcctgaa cccttactac tctcagtgcc tggaggccgc cgcccgcgag 1561 gccgccgccc gcgaggccgc cgcccgcgag gccgccgccc gcaagcgggg atgctctagg 1621 gatgctgata aggctacggc tacacctaca gctgagccga gtgagagtcc agatgtaaca 1681 aaggaacccg aagaaactca ggaaccagta gaaagcaaga gcccagagcc aaaggatgat 1741 ccaaagacca gtgaaatcaa aatacctgac ctttccatag aggcatttga tattccagat 1801 acagatagcc aggcctttgt taacaatatg aagcttggtt ggaacctagg caataccttt 1861 gatgcccatg actgtacctg gctttccaac aagttggatt atgagaaggg ctggtcaggg 1921 ataaagacta ctgaagagat gatccaggca gtaaaggatg ccggctttaa cactgtacgt 1981 ataccagtat cctggcataa ccatgtctca ggcgatgacc acaccataga ccaggcctgg 2041 ctagacaggg tccaggaggt agtagactat gctatagaca atggccttta tgccattatc 2101 aatatacatc acgatatggg tgaggacttt atttatccaa gtagccaata catggaccag 2161 tcctcccatt atgtaaggcg tatttggacc cagctagctg aaagatttaa ggattatgac 2221 gaacacctaa tatttgaatc cttaaatgag ccccggatgg taggcagtaa gtatgagtgg 2281 tggctagatt caaatgatga gagctgcaag gatgcagtag ctagcatcaa caagcttaac 2341 caaatatttg tcgatactgt aagggccagt ggtgctaata ataagagccg ctatcttatg 2401 gtaccaggct acgcagcctc agtggaagga gcacttaata agggctttga gcttcccaag 2461 gatagggtag aggatagact aatcctttcc atacatgcct atacccctta caattttgcc 2521 ttagaggctg gaggggttag tagctttgac attaataagt ccagcagtgt cagggagata 2581 gaccagttta tgacccagct gcacgagaaa tttgtttccc aggcagtccc agttgtaata 2641 ggtgagtttg gtgctaggaa taaaaagaac aaccttcagg acagggtgga tttctctgca 2701 tattacgtgg catcagccag ggccaggggc atgacctgca tatggtggga caaccatgcc 2761 tttactggag acggtgagct ctttggtatc ctagacaggt ccaaatcaag ctggaagtac 2821 ccagagattg tagaggctat gttaaaatac gctgaccatc atcatcatca tcattgatac 2881 tagagatcga acg //