BBa_J212100
1
BBa_J212100
test
2012-02-03T12:00:00Z
2015-08-31T04:08:37Z
home-made
This is test only.
true
false
_921_
0
11311
9
Discontinued
false
what?
false
Shuangxi Ren
component2170108
1
BBa_J212099
component2170106
1
BBa_J212006
annotation2170108
1
BBa_J212099
range2170108
1
2886
2893
annotation2170106
1
BBa_J212006
range2170106
1
1
2877
BBa_J212099
1
BBa_J212099
test
2012-02-03T12:00:00Z
2015-08-31T04:08:37Z
home-made
this is a test.
true
false
_921_
0
11311
9
Discontinued
false
rfc10
false
Shuangxi Ren
annotation2170086
1
rbs
range2170086
1
1
5
BBa_J212006
1
Tc2bh
Exo2b catalytic domain fused with the cbh1 gene
2012-02-03T12:00:00Z
2015-08-31T04:08:37Z
Metagenomic fosmid library of a biogas digester fed with pig manure and rice straw
A novel GH5 cellulase gene exo2b was identified from a metagenomic library. The fragment encoding the Exo2b catalytic domain was then fused with the cbh1 gene from Trichoderma reesei, and the fused gene was successfully expressed in T. reesei Rut-C30 .Compared to that of the parent strain, the filter paper activity (FPA) and CMCase activity of the secreted proteins of a selected transformant A1, increased 24% and 18%, respectively.
false
false
_921_
0
11112
9
Not in stock
false
The foreign gene exo2b wasfused with the native gene cbh1 to facilitate the translocation of the foreign protein in the secretory pathway of Trichoderma reesei and avoid degradatio
false
Xing Yan
annotation2170096
1
KR site
range2170096
1
1602
1608
annotation2170094
1
cbh1
range2170094
1
1
1542
annotation2170098
1
His tag
range2170098
1
2857
2874
annotation2170097
1
exo2b core
range2170097
1
1609
2856
annotation2170095
1
linker
range2170095
1
1543
1602
annotation2170099
1
stop codon
range2170099
1
2875
2877
BBa_J212099_sequence
1
atcgaacg
BBa_J212006_sequence
1
atgtatcggaagttggccgtcatcacggccttcttggccacagctcgtgctcagtcggcctgcactctccaatcggagactcacccgcctctgacatggcagaaatgctcgtctggtggcacgtgcactcaacagacaggctccgtggtcatcgacgccaactggcgctggactcacgctacgaacagcagcacgaactgctacgatggcaacacttggagctcgaccctatgtcctgacaacgagacctgcgcgaagaactgctgtctggacggtgccgcctacgcgtccacgtacggagttaccacgagcggtaacagcctctccattggctttgtcacccagtctgcgcagaagaacgttggcgctcgcctttaccttatggcgagcgacacgacctaccaggaattcaccctgcttggcaacgagttctctttcgatgttgatgtttcgcagctgccgtgcggcttgaacggagctctctacttcgtgtccatggacgcggatggtggcgtgagcaagtatcccaccaacaccgctggcgccaagtacggcacggggtactgtgacagccagtgtccccgcgatctgaagttcatcaatggccaggccaacgttgagggctgggagccgtcatccaacaacgcgaacacgggcattggaggacacggaagctgctgctctgagatggatatctgggaggccaactccatctccgaggctcttaccccccacccttgcacgactgtcggccaggagatctgtgagggtgatgggtgcggcggaacttattccgatgacagatatggcggcacttgcgatcccgatggctgcgactggaacccataccgcctgggcaacaccagcttctacggccctggctcaagctttaccctcgataccaccaagaaattgaccgttgtcacccagttcgagacgtcgggtgccatcaaccgatactatgtccagaatggcgtcactttccagcagcccaacgccgagcttggtagttactctggcaacgagctcaacgatgattactgcacagctgaggaggcagaattcggcggatcctctttctcagacaagggcggcctgactcagttcaagaaggctacctctggcggcatggttctggtcatgagtctgtgggatgattactacgccaacatgctgtggctggactccacctacccgacaaacgagacctcctccacacccggtgccgtgcgcggaagctgctccaccagctccggtgtccctgctcaggtcgaatctcagtctcccaacgccaaggtcaccttctccaacatcaagttcggacccattggcagcaccggcaaccctagcggcggcaaccctcccggcggaaacccgcctggcaccaccaccacccgccgcccagccactaccactggaagctctcccggacctactcagtctcactacggccagtgcggcggtattggctacagcggccccacggtctgcgccagcggcacaacttgccaggtcctgaacccttactactctcagtgcctggaggccgccgcccgcgaggccgccgcccgcgaggccgccgcccgcgaggccgccgcccgcaagcggggatgctctagggatgctgataaggctacggctacacctacagctgagccgagtgagagtccagatgtaacaaaggaacccgaagaaactcaggaaccagtagaaagcaagagcccagagccaaaggatgatccaaagaccagtgaaatcaaaatacctgacctttccatagaggcatttgatattccagatacagatagccaggcctttgttaacaatatgaagcttggttggaacctaggcaatacctttgatgcccatgactgtacctggctttccaacaagttggattatgagaagggctggtcagggataaagactactgaagagatgatccaggcagtaaaggatgccggctttaacactgtacgtataccagtatcctggcataaccatgtctcaggcgatgaccacaccatagaccaggcctggctagacagggtccaggaggtagtagactatgctatagacaatggcctttatgccattatcaatatacatcacgatatgggtgaggactttatttatccaagtagccaatacatggaccagtcctcccattatgtaaggcgtatttggacccagctagctgaaagatttaaggattatgacgaacacctaatatttgaatccttaaatgagccccggatggtaggcagtaagtatgagtggtggctagattcaaatgatgagagctgcaaggatgcagtagctagcatcaacaagcttaaccaaatatttgtcgatactgtaagggccagtggtgctaataataagagccgctatcttatggtaccaggctacgcagcctcagtggaaggagcacttaataagggctttgagcttcccaaggatagggtagaggatagactaatcctttccatacatgcctataccccttacaattttgccttagaggctggaggggttagtagctttgacattaataagtccagcagtgtcagggagatagaccagtttatgacccagctgcacgagaaatttgtttcccaggcagtcccagttgtaataggtgagtttggtgctaggaataaaaagaacaaccttcaggacagggtggatttctctgcatattacgtggcatcagccagggccaggggcatgacctgcatatggtgggacaaccatgcctttactggagacggtgagctctttggtatcctagacaggtccaaatcaagctggaagtacccagagattgtagaggctatgttaaaatacgctgaccatcatcatcatcatcattga
BBa_J212100_sequence
1
atgtatcggaagttggccgtcatcacggccttcttggccacagctcgtgctcagtcggcctgcactctccaatcggagactcacccgcctctgacatggcagaaatgctcgtctggtggcacgtgcactcaacagacaggctccgtggtcatcgacgccaactggcgctggactcacgctacgaacagcagcacgaactgctacgatggcaacacttggagctcgaccctatgtcctgacaacgagacctgcgcgaagaactgctgtctggacggtgccgcctacgcgtccacgtacggagttaccacgagcggtaacagcctctccattggctttgtcacccagtctgcgcagaagaacgttggcgctcgcctttaccttatggcgagcgacacgacctaccaggaattcaccctgcttggcaacgagttctctttcgatgttgatgtttcgcagctgccgtgcggcttgaacggagctctctacttcgtgtccatggacgcggatggtggcgtgagcaagtatcccaccaacaccgctggcgccaagtacggcacggggtactgtgacagccagtgtccccgcgatctgaagttcatcaatggccaggccaacgttgagggctgggagccgtcatccaacaacgcgaacacgggcattggaggacacggaagctgctgctctgagatggatatctgggaggccaactccatctccgaggctcttaccccccacccttgcacgactgtcggccaggagatctgtgagggtgatgggtgcggcggaacttattccgatgacagatatggcggcacttgcgatcccgatggctgcgactggaacccataccgcctgggcaacaccagcttctacggccctggctcaagctttaccctcgataccaccaagaaattgaccgttgtcacccagttcgagacgtcgggtgccatcaaccgatactatgtccagaatggcgtcactttccagcagcccaacgccgagcttggtagttactctggcaacgagctcaacgatgattactgcacagctgaggaggcagaattcggcggatcctctttctcagacaagggcggcctgactcagttcaagaaggctacctctggcggcatggttctggtcatgagtctgtgggatgattactacgccaacatgctgtggctggactccacctacccgacaaacgagacctcctccacacccggtgccgtgcgcggaagctgctccaccagctccggtgtccctgctcaggtcgaatctcagtctcccaacgccaaggtcaccttctccaacatcaagttcggacccattggcagcaccggcaaccctagcggcggcaaccctcccggcggaaacccgcctggcaccaccaccacccgccgcccagccactaccactggaagctctcccggacctactcagtctcactacggccagtgcggcggtattggctacagcggccccacggtctgcgccagcggcacaacttgccaggtcctgaacccttactactctcagtgcctggaggccgccgcccgcgaggccgccgcccgcgaggccgccgcccgcgaggccgccgcccgcaagcggggatgctctagggatgctgataaggctacggctacacctacagctgagccgagtgagagtccagatgtaacaaaggaacccgaagaaactcaggaaccagtagaaagcaagagcccagagccaaaggatgatccaaagaccagtgaaatcaaaatacctgacctttccatagaggcatttgatattccagatacagatagccaggcctttgttaacaatatgaagcttggttggaacctaggcaatacctttgatgcccatgactgtacctggctttccaacaagttggattatgagaagggctggtcagggataaagactactgaagagatgatccaggcagtaaaggatgccggctttaacactgtacgtataccagtatcctggcataaccatgtctcaggcgatgaccacaccatagaccaggcctggctagacagggtccaggaggtagtagactatgctatagacaatggcctttatgccattatcaatatacatcacgatatgggtgaggactttatttatccaagtagccaatacatggaccagtcctcccattatgtaaggcgtatttggacccagctagctgaaagatttaaggattatgacgaacacctaatatttgaatccttaaatgagccccggatggtaggcagtaagtatgagtggtggctagattcaaatgatgagagctgcaaggatgcagtagctagcatcaacaagcttaaccaaatatttgtcgatactgtaagggccagtggtgctaataataagagccgctatcttatggtaccaggctacgcagcctcagtggaaggagcacttaataagggctttgagcttcccaaggatagggtagaggatagactaatcctttccatacatgcctataccccttacaattttgccttagaggctggaggggttagtagctttgacattaataagtccagcagtgtcagggagatagaccagtttatgacccagctgcacgagaaatttgtttcccaggcagtcccagttgtaataggtgagtttggtgctaggaataaaaagaacaaccttcaggacagggtggatttctctgcatattacgtggcatcagccagggccaggggcatgacctgcatatggtgggacaaccatgcctttactggagacggtgagctctttggtatcctagacaggtccaaatcaagctggaagtacccagagattgtagaggctatgttaaaatacgctgaccatcatcatcatcatcattgatactagagatcgaacg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z