LOCUS       BBa_J22001              2630 bp    DNA     linear       22-Nov-2024
DEFINITION  Lac promoter-Tar Receptor-CFP reporter
ACCESSION   BBa_J22001
VERSION     BBa_J22001.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     RBS             64..76
                     /label=RBS-3\Weak
     RBS             70..73
                     /label=RBS
     misc_feature    64..76
                     /label=BBa_B0032
     RBS             64..76
                     /label=BBa_B0032
     CDS             83..1744
                     /label=BBa_C0600
     misc_feature    1757..1760
                     /label=conserved
     RBS             1753..1764
                     /label=BBa_B0034
     CDS             1771..2493
                     /label=BBa_E0020
     stem_loop       2513..2556
                     /label=stem_loop
     misc_feature    2502..2581
                     /label=BBa_B0010
     terminator      2502..2581
                     /label=BBa_B0010
     stem_loop       2597..2616
                     /label=T7 TE
     misc_feature    2623..2623
                     /label=stop
     polyA_site      2617..2630
                     /label=polya
     misc_feature    2590..2630
                     /label=BBa_B0012
     terminator      2590..2630
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagtcacaca ggaaagtact agatgattaa ccgtatccgc gtagtcacgc tgttggtaat
      121 ggtgctgggg gtattcgcac tgttacagct tatttccggc agtctgtttt tttcttccct
      181 tcaccatagc cagaagagct ttgtggtttc caatcaatta cgggaacagc agggcgagct
      241 gacgtcaacc tgggatttaa tgctgcaaac gcgcattaac ctgagtcgtt cagcggtacg
      301 gatgatgatg gattcctcca atcaacaaag taacgccaaa gttgaattgc tcgatagcgc
      361 caggaaaaca ttggcgcagg cagcgacgca ttataaaaaa ttcaaaagca tggcaccgtt
      421 acctgaaatg gtcgctacca gtcgtaatat tgatgaaaaa tataaaaact attacacagc
      481 gttaactgaa ctgattgatt acctagatta tggcaatact ggagcttatt tcgctcagcc
      541 aacccaggga atgcaaaatg caatgggcga agcgtttgct cagtacgccc tcagcagtga
      601 aaaactgtat cgcgatatcg tcactgacaa cgcagatgat taccgatttg cccagtggca
      661 actggcggtt atcgcgctgg tggtggtatt gattctgctg gtggcgtggt acggcattcg
      721 ccgtatgttg cttactccgc tggcaaaaat tattgctcac attcgcgaaa tcgccggtgg
      781 taacctggcg aataccctga ccattgacgg gcgcagtgaa atgggcgacc tggcgcagag
      841 cgtttcacat atgcaacgct ctttgactga caccgtcact catgtccgcg aaggttcaga
      901 tgccatctat gccggtaccc gtgaaattgc ggcgggcaac accgatcttt cctcccgtac
      961 tgaacagcag gcatccgcgc tggaagaaac tgccgccagc atggagcagc tcaccgcgac
     1021 agtgaagcaa aacgccgata acgcccgcca ggcctcgcaa ctggcgcaaa gtgcctccga
     1081 caccgcccag cacggcggca aagtggtgga tggcgtagtg aaaacgatgc atgagatcgc
     1141 cgatagttcg aagaaaattg ccgacattat cagcgttatc gacggtattg ccttccagac
     1201 taatatcctc gcgctgaatg ccgcggttga agccgcgcgt gcgggtgaac agggccgtgg
     1261 ttttgccgtg gtggcgggtg aagtgcgtaa tcttgccagt cgcagcgccc aggcggcaaa
     1321 agagatcaaa gccctcattg aagactccgt ctcacgcgtt gataccggtt cggtgctggt
     1381 cgaaagcgcc ggggaaacaa tgaacaatat cgtcaatgct gtcactcgcg tgactgacat
     1441 tatgggcgag attgcatcgg catcggatga acagagccgt ggcatcgatc aagtcgcatt
     1501 ggcggtttcg gaaatggatc gcgtcacgca acagaacgca tcgctggtgc aggaatcagc
     1561 tgccgccgcc gctgcgctgg aagaacaggc gagtcgttta acgcaagcag tttccgcgtt
     1621 ccgtctggca gccagcccac tcaccaataa accgcaaaca ccatcccgtc ctgccagtga
     1681 gcaaccaccg gctcagccac gactgcgaat tgctgaacaa gatccaaact gggaaacatt
     1741 ttgatactag agaaagagga gaaatactag atggtgagca agggcgagga gctgttcacc
     1801 ggggtggtgc ccatcctggt cgagctggac ggcgacgtga acggccacaa gttcagcgtg
     1861 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     1921 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag
     1981 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     2041 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     2101 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     2161 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacat cagccacaac
     2221 gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac
     2281 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     2341 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     2401 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     2461 actctcggca tggacgagct gtacaagtaa taatactaga gccaggcatc aaataaaacg
     2521 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     2581 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//