LOCUS       BBa_J22005              2623 bp    DNA     linear       22-Dec-2024
DEFINITION  Tet promoter-Tsr receptor-RFP Reporter
ACCESSION   BBa_J22005
VERSION     BBa_J22005.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..75
                     /label=RBS-3\Weak
     RBS             69..72
                     /label=RBS
     misc_feature    63..75
                     /label=BBa_B0032
     RBS             63..75
                     /label=BBa_B0032
     CDS             82..1737
                     /label=BBa_C0620
     misc_feature    1750..1753
                     /label=conserved
     RBS             1746..1757
                     /label=BBa_B0034
     CDS             1764..2486
                     /label=BBa_E0030
     stem_loop       2506..2549
                     /label=stem_loop
     misc_feature    2495..2574
                     /label=BBa_B0010
     terminator      2495..2574
                     /label=BBa_B0010
     stem_loop       2590..2609
                     /label=T7 TE
     misc_feature    2616..2616
                     /label=stop
     polyA_site      2610..2623
                     /label=polya
     misc_feature    2583..2623
                     /label=BBa_B0012
     terminator      2583..2623
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gaaagtacta gatgttaaaa cgtatcaaaa ttgtgaccag cttactgctg
      121 gttttggccg tttttggcct tttacaactg acatcaggcg gtctgttctt taatgcctta
      181 aagaatgaca aagaaaattt cactgtttta caaaccattc gccagcagca atccacgctg
      241 aatggcagct gggtcgcgtt gttgcagacg cgtaacaccc tcaaccgcgc gggtatccgc
      301 tacatgatgg atcagaataa tattggtagc ggttcaaccg ttgctgagct gatggagagt
      361 gccagtattt cgctgaaaca ggcggaaaaa aactgggcgg attacgaagc gttgccgcgt
      421 gacccgcgtc agagcaccgc cgcagcggca gagatcaaac gtaattacga tatttatcac
      481 aatgcgctgg cggagctgat ccaactgtta ggtgcaggca aaatcaacga gttctttgat
      541 cagccgaccc agggatatca ggacggtttc gagaagcagt atgtggctta catggagcaa
      601 aacgatcggc tccatgatat cgccgtcagc gataacaatg cctcctacag ccaggcgatg
      661 tggattctgg tgggcgtgat gatcgtcgta ctggcggtca tcttcgccgt ctggttcggt
      721 attaaagcct cgctggtagc gccaatgaat cgcctgattg acagcattcg tcatattgca
      781 ggcggcgatc tggtgaaacc gattgaggtg gatggctcta atgagatggg gcaactggca
      841 gagagtttgc gccatatgca gggagagctg atgcgtaccg tcggtgatgt gcgcaacggg
      901 gccaatgcca tctatagcgg tgccagcgaa atcgccaccg gcaataacga tctctcttcg
      961 cgcaccgagc aacaggccgc ttcgctggaa gagacggcag ccagcatgga gcaactgacc
     1021 gcaacggtga agcagaacgc cgaaaatgcg cgccaggcca gccatctggc gttaagtgct
     1081 tctgaaacgg cgcaacgcgg cggtaaagtg gtagataacg tggtgcagac tatgcgcgat
     1141 atctccacca gttcgcagaa aatcgccgat attatcagcg taattgacgg cattgccttc
     1201 cagaccaata ttctggcttt gaacgcggcg gttgaggctg cgcgtgcggg tgagcaaggg
     1261 cgcggttttg cggtggtcgc gggagaagtg cgtaatctgg cccagcgcag cgcccaggcg
     1321 gctcgtgaaa ttaaaagcct gattgaagac tcggtgggga aagtggatgt tggctctacg
     1381 ctggtcgaaa gcgccgggga aacaatggcg gagattgtca gcgccgtgac ccgcgtgacg
     1441 gacattatgg gcgaaattgc ttctgcttct gatgagcaga gccgtggtat cgatcaggtt
     1501 ggcttagcgg ttgctgagat ggaccgggta actcaacaga acgccgcgct ggtggaagag
     1561 tctgccgctg ccgccgccgc gctggaagag caggccagtc gcctgaccga agcagtggca
     1621 gtgttccgga ttcagcaaca gcagcgtgaa acatcggctg tggtaaaaac cgtgacgcca
     1681 gctgcgccgc gtaaaatggc cgtggcagat agcgaggaga actgggaaac attttaatac
     1741 tagagaaaga ggagaaatac tagatggtga gcaagggcga ggagctgttc accggggtgg
     1801 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     1861 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     1921 agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg caatgcttcg
     1981 cccgctaccc cgaccacatg aagctgcacg acttcttcaa gtccgccatg cccgaaggct
     2041 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     2101 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     2161 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     2221 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     2281 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     2341 ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca
     2401 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     2461 gcatggacga gctgtacaag taataatact agagccaggc atcaaataaa acgaaaggct
     2521 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     2581 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//