LOCUS BBa_J22010 5261 bp DNA linear 22-Nov-2024 DEFINITION Lac promoter-Tar Receptor-CFP reporter-Tet promoter-Tsr receptor-RFP reporter ACCESSION BBa_J22010 VERSION BBa_J22010.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 RBS 64..76 /label=RBS-3\Weak RBS 70..73 /label=RBS misc_feature 64..76 /label=BBa_B0032 RBS 64..76 /label=BBa_B0032 CDS 83..1744 /label=BBa_C0600 misc_feature 1757..1760 /label=conserved RBS 1753..1764 /label=BBa_B0034 CDS 1771..2493 /label=BBa_E0020 stem_loop 2513..2556 /label=stem_loop misc_feature 2502..2581 /label=BBa_B0010 terminator 2502..2581 /label=BBa_B0010 stem_loop 2597..2616 /label=T7 TE misc_feature 2623..2623 /label=stop polyA_site 2617..2630 /label=polya misc_feature 2590..2630 /label=BBa_B0012 terminator 2590..2630 /label=BBa_B0012 misc_feature 2639..2657 /label=TetR 1 misc_feature 2639..2692 /label=BBa_R0040 promoter 2658..2663 /label=-35 misc_feature 2664..2682 /label=TetR 2 promoter 2681..2686 /label=-10 promoter 2639..2692 /label=BBa_R0040 RBS 2701..2713 /label=RBS-3\Weak RBS 2707..2710 /label=RBS misc_feature 2701..2713 /label=BBa_B0032 RBS 2701..2713 /label=BBa_B0032 CDS 2720..4375 /label=BBa_C0620 misc_feature 4388..4391 /label=conserved RBS 4384..4395 /label=BBa_B0034 CDS 4402..5124 /label=BBa_E0030 stem_loop 5144..5187 /label=stem_loop misc_feature 5133..5212 /label=BBa_B0010 terminator 5133..5212 /label=BBa_B0010 stem_loop 5228..5247 /label=T7 TE misc_feature 5254..5254 /label=stop polyA_site 5248..5261 /label=polya misc_feature 5221..5261 /label=BBa_B0012 terminator 5221..5261 /label=BBa_B0012 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagtcacaca ggaaagtact agatgattaa ccgtatccgc gtagtcacgc tgttggtaat 121 ggtgctgggg gtattcgcac tgttacagct tatttccggc agtctgtttt tttcttccct 181 tcaccatagc cagaagagct ttgtggtttc caatcaatta cgggaacagc agggcgagct 241 gacgtcaacc tgggatttaa tgctgcaaac gcgcattaac ctgagtcgtt cagcggtacg 301 gatgatgatg gattcctcca atcaacaaag taacgccaaa gttgaattgc tcgatagcgc 361 caggaaaaca ttggcgcagg cagcgacgca ttataaaaaa ttcaaaagca tggcaccgtt 421 acctgaaatg gtcgctacca gtcgtaatat tgatgaaaaa tataaaaact attacacagc 481 gttaactgaa ctgattgatt acctagatta tggcaatact ggagcttatt tcgctcagcc 541 aacccaggga atgcaaaatg caatgggcga agcgtttgct cagtacgccc tcagcagtga 601 aaaactgtat cgcgatatcg tcactgacaa cgcagatgat taccgatttg cccagtggca 661 actggcggtt atcgcgctgg tggtggtatt gattctgctg gtggcgtggt acggcattcg 721 ccgtatgttg cttactccgc tggcaaaaat tattgctcac attcgcgaaa tcgccggtgg 781 taacctggcg aataccctga ccattgacgg gcgcagtgaa atgggcgacc tggcgcagag 841 cgtttcacat atgcaacgct ctttgactga caccgtcact catgtccgcg aaggttcaga 901 tgccatctat gccggtaccc gtgaaattgc ggcgggcaac accgatcttt cctcccgtac 961 tgaacagcag gcatccgcgc tggaagaaac tgccgccagc atggagcagc tcaccgcgac 1021 agtgaagcaa aacgccgata acgcccgcca ggcctcgcaa ctggcgcaaa gtgcctccga 1081 caccgcccag cacggcggca aagtggtgga tggcgtagtg aaaacgatgc atgagatcgc 1141 cgatagttcg aagaaaattg ccgacattat cagcgttatc gacggtattg ccttccagac 1201 taatatcctc gcgctgaatg ccgcggttga agccgcgcgt gcgggtgaac agggccgtgg 1261 ttttgccgtg gtggcgggtg aagtgcgtaa tcttgccagt cgcagcgccc aggcggcaaa 1321 agagatcaaa gccctcattg aagactccgt ctcacgcgtt gataccggtt cggtgctggt 1381 cgaaagcgcc ggggaaacaa tgaacaatat cgtcaatgct gtcactcgcg tgactgacat 1441 tatgggcgag attgcatcgg catcggatga acagagccgt ggcatcgatc aagtcgcatt 1501 ggcggtttcg gaaatggatc gcgtcacgca acagaacgca tcgctggtgc aggaatcagc 1561 tgccgccgcc gctgcgctgg aagaacaggc gagtcgttta acgcaagcag tttccgcgtt 1621 ccgtctggca gccagcccac tcaccaataa accgcaaaca ccatcccgtc ctgccagtga 1681 gcaaccaccg gctcagccac gactgcgaat tgctgaacaa gatccaaact gggaaacatt 1741 ttgatactag agaaagagga gaaatactag atggtgagca agggcgagga gctgttcacc 1801 ggggtggtgc ccatcctggt cgagctggac ggcgacgtga acggccacaa gttcagcgtg 1861 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc 1921 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag 1981 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc 2041 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc 2101 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac 2161 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacat cagccacaac 2221 gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac 2281 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc 2341 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa 2401 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc 2461 actctcggca tggacgagct gtacaagtaa taatactaga gccaggcatc aaataaaacg 2521 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 2581 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagtc 2641 cctatcagtg atagagattg acatccctat cagtgataga gatactgagc actactagag 2701 tcacacagga aagtactaga tgttaaaacg tatcaaaatt gtgaccagct tactgctggt 2761 tttggccgtt tttggccttt tacaactgac atcaggcggt ctgttcttta atgccttaaa 2821 gaatgacaaa gaaaatttca ctgttttaca aaccattcgc cagcagcaat ccacgctgaa 2881 tggcagctgg gtcgcgttgt tgcagacgcg taacaccctc aaccgcgcgg gtatccgcta 2941 catgatggat cagaataata ttggtagcgg ttcaaccgtt gctgagctga tggagagtgc 3001 cagtatttcg ctgaaacagg cggaaaaaaa ctgggcggat tacgaagcgt tgccgcgtga 3061 cccgcgtcag agcaccgccg cagcggcaga gatcaaacgt aattacgata tttatcacaa 3121 tgcgctggcg gagctgatcc aactgttagg tgcaggcaaa atcaacgagt tctttgatca 3181 gccgacccag ggatatcagg acggtttcga gaagcagtat gtggcttaca tggagcaaaa 3241 cgatcggctc catgatatcg ccgtcagcga taacaatgcc tcctacagcc aggcgatgtg 3301 gattctggtg ggcgtgatga tcgtcgtact ggcggtcatc ttcgccgtct ggttcggtat 3361 taaagcctcg ctggtagcgc caatgaatcg cctgattgac agcattcgtc atattgcagg 3421 cggcgatctg gtgaaaccga ttgaggtgga tggctctaat gagatggggc aactggcaga 3481 gagtttgcgc catatgcagg gagagctgat gcgtaccgtc ggtgatgtgc gcaacggggc 3541 caatgccatc tatagcggtg ccagcgaaat cgccaccggc aataacgatc tctcttcgcg 3601 caccgagcaa caggccgctt cgctggaaga gacggcagcc agcatggagc aactgaccgc 3661 aacggtgaag cagaacgccg aaaatgcgcg ccaggccagc catctggcgt taagtgcttc 3721 tgaaacggcg caacgcggcg gtaaagtggt agataacgtg gtgcagacta tgcgcgatat 3781 ctccaccagt tcgcagaaaa tcgccgatat tatcagcgta attgacggca ttgccttcca 3841 gaccaatatt ctggctttga acgcggcggt tgaggctgcg cgtgcgggtg agcaagggcg 3901 cggttttgcg gtggtcgcgg gagaagtgcg taatctggcc cagcgcagcg cccaggcggc 3961 tcgtgaaatt aaaagcctga ttgaagactc ggtggggaaa gtggatgttg gctctacgct 4021 ggtcgaaagc gccggggaaa caatggcgga gattgtcagc gccgtgaccc gcgtgacgga 4081 cattatgggc gaaattgctt ctgcttctga tgagcagagc cgtggtatcg atcaggttgg 4141 cttagcggtt gctgagatgg accgggtaac tcaacagaac gccgcgctgg tggaagagtc 4201 tgccgctgcc gccgccgcgc tggaagagca ggccagtcgc ctgaccgaag cagtggcagt 4261 gttccggatt cagcaacagc agcgtgaaac atcggctgtg gtaaaaaccg tgacgccagc 4321 tgcgccgcgt aaaatggccg tggcagatag cgaggagaac tgggaaacat tttaatacta 4381 gagaaagagg agaaatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg 4441 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag 4501 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag 4561 ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca atgcttcgcc 4621 cgctaccccg accacatgaa gctgcacgac ttcttcaagt ccgccatgcc cgaaggctac 4681 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg 4741 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag 4801 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc 4861 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag 4921 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc 4981 gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac 5041 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc 5101 atggacgagc tgtacaagta ataatactag agccaggcat caaataaaac gaaaggctca 5161 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 5221 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a //