##gff-version 3
##sequence-region BBa_J22010 1 5261
BBa_J22010	.	terminator	5221	5261	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J22010	.	engineered_region	5221	5261	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J22010	.	stop_codon	5254	5254	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J22010	.	stem_loop	5228	5247	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J22010	.	polyA_site	5248	5261	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J22010	.	CDS	83	1744	.	+	0	ID=BBa_C0600;Name=BBa_C0600
BBa_J22010	.	ribosome_entry_site	2701	2713	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_J22010	.	ribosome_entry_site	2707	2710	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J22010	.	engineered_region	2701	2713	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J22010	.	ribosome_entry_site	2701	2713	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J22010	.	promoter	2639	2692	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J22010	.	promoter	2658	2663	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J22010	.	non_covalent_binding_site	2664	2682	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J22010	.	engineered_region	2639	2692	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J22010	.	non_covalent_binding_site	2639	2657	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J22010	.	promoter	2681	2686	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J22010	.	CDS	2720	4375	.	+	0	ID=BBa_C0620;Name=BBa_C0620
BBa_J22010	.	ribosome_entry_site	1753	1764	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J22010	.	conserved_region	1757	1760	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J22010	.	terminator	5133	5212	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J22010	.	stem_loop	5144	5187	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J22010	.	engineered_region	5133	5212	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J22010	.	terminator	2590	2630	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J22010	.	engineered_region	2590	2630	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J22010	.	stop_codon	2623	2623	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J22010	.	stem_loop	2597	2616	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J22010	.	polyA_site	2617	2630	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J22010	.	CDS	4402	5124	.	+	0	ID=BBa_E0030;Name=BBa_E0030
BBa_J22010	.	terminator	2502	2581	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J22010	.	stem_loop	2513	2556	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J22010	.	engineered_region	2502	2581	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J22010	.	ribosome_entry_site	4384	4395	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J22010	.	conserved_region	4388	4391	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J22010	.	ribosome_entry_site	64	76	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_J22010	.	ribosome_entry_site	70	73	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J22010	.	engineered_region	64	76	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J22010	.	ribosome_entry_site	64	76	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J22010	.	CDS	1771	2493	.	+	0	ID=BBa_E0020;Name=BBa_E0020
BBa_J22010	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_J22010	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J22010	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J22010	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J22010	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J22010	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
>BBa_J22010
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagtcacacaggaaagtac
tagatgattaaccgtatccgcgtagtcacgctgttggtaatggtgctgggggtattcgcactgttacagcttatttccg
gcagtctgtttttttcttcccttcaccatagccagaagagctttgtggtttccaatcaattacgggaacagcagggcga
gctgacgtcaacctgggatttaatgctgcaaacgcgcattaacctgagtcgttcagcggtacggatgatgatggattcc
tccaatcaacaaagtaacgccaaagttgaattgctcgatagcgccaggaaaacattggcgcaggcagcgacgcattata
aaaaattcaaaagcatggcaccgttacctgaaatggtcgctaccagtcgtaatattgatgaaaaatataaaaactatta
cacagcgttaactgaactgattgattacctagattatggcaatactggagcttatttcgctcagccaacccagggaatg
caaaatgcaatgggcgaagcgtttgctcagtacgccctcagcagtgaaaaactgtatcgcgatatcgtcactgacaacg
cagatgattaccgatttgcccagtggcaactggcggttatcgcgctggtggtggtattgattctgctggtggcgtggta
cggcattcgccgtatgttgcttactccgctggcaaaaattattgctcacattcgcgaaatcgccggtggtaacctggcg
aataccctgaccattgacgggcgcagtgaaatgggcgacctggcgcagagcgtttcacatatgcaacgctctttgactg
acaccgtcactcatgtccgcgaaggttcagatgccatctatgccggtacccgtgaaattgcggcgggcaacaccgatct
ttcctcccgtactgaacagcaggcatccgcgctggaagaaactgccgccagcatggagcagctcaccgcgacagtgaag
caaaacgccgataacgcccgccaggcctcgcaactggcgcaaagtgcctccgacaccgcccagcacggcggcaaagtgg
tggatggcgtagtgaaaacgatgcatgagatcgccgatagttcgaagaaaattgccgacattatcagcgttatcgacgg
tattgccttccagactaatatcctcgcgctgaatgccgcggttgaagccgcgcgtgcgggtgaacagggccgtggtttt
gccgtggtggcgggtgaagtgcgtaatcttgccagtcgcagcgcccaggcggcaaaagagatcaaagccctcattgaag
actccgtctcacgcgttgataccggttcggtgctggtcgaaagcgccggggaaacaatgaacaatatcgtcaatgctgt
cactcgcgtgactgacattatgggcgagattgcatcggcatcggatgaacagagccgtggcatcgatcaagtcgcattg
gcggtttcggaaatggatcgcgtcacgcaacagaacgcatcgctggtgcaggaatcagctgccgccgccgctgcgctgg
aagaacaggcgagtcgtttaacgcaagcagtttccgcgttccgtctggcagccagcccactcaccaataaaccgcaaac
accatcccgtcctgccagtgagcaaccaccggctcagccacgactgcgaattgctgaacaagatccaaactgggaaaca
ttttgatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcct
ggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaag
ctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcg
tgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtcca
ggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtg
aaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatca
gccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgagga
cggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccac
tacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccg
ccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctc
agtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttc
gggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatact
gagcactactagagtcacacaggaaagtactagatgttaaaacgtatcaaaattgtgaccagcttactgctggttttgg
ccgtttttggccttttacaactgacatcaggcggtctgttctttaatgccttaaagaatgacaaagaaaatttcactgt
tttacaaaccattcgccagcagcaatccacgctgaatggcagctgggtcgcgttgttgcagacgcgtaacaccctcaac
cgcgcgggtatccgctacatgatggatcagaataatattggtagcggttcaaccgttgctgagctgatggagagtgcca
gtatttcgctgaaacaggcggaaaaaaactgggcggattacgaagcgttgccgcgtgacccgcgtcagagcaccgccgc
agcggcagagatcaaacgtaattacgatatttatcacaatgcgctggcggagctgatccaactgttaggtgcaggcaaa
atcaacgagttctttgatcagccgacccagggatatcaggacggtttcgagaagcagtatgtggcttacatggagcaaa
acgatcggctccatgatatcgccgtcagcgataacaatgcctcctacagccaggcgatgtggattctggtgggcgtgat
gatcgtcgtactggcggtcatcttcgccgtctggttcggtattaaagcctcgctggtagcgccaatgaatcgcctgatt
gacagcattcgtcatattgcaggcggcgatctggtgaaaccgattgaggtggatggctctaatgagatggggcaactgg
cagagagtttgcgccatatgcagggagagctgatgcgtaccgtcggtgatgtgcgcaacggggccaatgccatctatag
cggtgccagcgaaatcgccaccggcaataacgatctctcttcgcgcaccgagcaacaggccgcttcgctggaagagacg
gcagccagcatggagcaactgaccgcaacggtgaagcagaacgccgaaaatgcgcgccaggccagccatctggcgttaa
gtgcttctgaaacggcgcaacgcggcggtaaagtggtagataacgtggtgcagactatgcgcgatatctccaccagttc
gcagaaaatcgccgatattatcagcgtaattgacggcattgccttccagaccaatattctggctttgaacgcggcggtt
gaggctgcgcgtgcgggtgagcaagggcgcggttttgcggtggtcgcgggagaagtgcgtaatctggcccagcgcagcg
cccaggcggctcgtgaaattaaaagcctgattgaagactcggtggggaaagtggatgttggctctacgctggtcgaaag
cgccggggaaacaatggcggagattgtcagcgccgtgacccgcgtgacggacattatgggcgaaattgcttctgcttct
gatgagcagagccgtggtatcgatcaggttggcttagcggttgctgagatggaccgggtaactcaacagaacgccgcgc
tggtggaagagtctgccgctgccgccgccgcgctggaagagcaggccagtcgcctgaccgaagcagtggcagtgttccg
gattcagcaacagcagcgtgaaacatcggctgtggtaaaaaccgtgacgccagctgcgccgcgtaaaatggccgtggca
gatagcgaggagaactgggaaacattttaatactagagaaagaggagaaatactagatggtgagcaagggcgaggagct
gttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggc
gagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccc
tcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtc
cgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtg
aagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggc
acaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaactt
caagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggc
cccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcaca
tggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagcca
ggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcta
ctagagtcacactggctcaccttcgggtgggcctttctgcgtttata