LOCUS       BBa_J22066              2888 bp    DNA     linear       25-Nov-2024
DEFINITION  pBAD luxR mRFP
ACCESSION   BBa_J22066
VERSION     BBa_J22066.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     RBS             1219..1231
                     /label=RBS-3\Weak
     RBS             1225..1228
                     /label=RBS
     misc_feature    1219..1231
                     /label=BBa_B0032
     RBS             1219..1231
                     /label=BBa_B0032
     misc_feature    1238..1239
                     /label=prefix
     misc_feature    1411..1411
                     /label=T
     misc_feature    1729..1729
                     /label=A
     CDS             1238..1987
                     /label=luxR
     misc_feature    1238..1993
                     /label=BBa_C0062
     misc_feature    1994..2018
                     /label=Help:Barcodes
     CDS             1238..1993
                     /label=BBa_C0062
     RBS             2027..2039
                     /label=RBS-3\Weak
     RBS             2033..2036
                     /label=RBS
     misc_feature    2027..2039
                     /label=BBa_B0032
     RBS             2027..2039
                     /label=BBa_B0032
     CDS             2046..2720
                     /label=mrfp1
     misc_feature    2727..2751
                     /label=Help:Barcodes
     CDS             2046..2751
                     /label=BBa_E1010
     stem_loop       2771..2814
                     /label=stem_loop
     misc_feature    2760..2839
                     /label=BBa_B0010
     terminator      2760..2839
                     /label=BBa_B0010
     stem_loop       2855..2874
                     /label=T7 TE
     misc_feature    2881..2881
                     /label=stop
     polyA_site      2875..2888
                     /label=polya
     misc_feature    2848..2888
                     /label=BBa_B0012
     terminator      2848..2888
                     /label=BBa_B0012
     terminator      2760..2888
                     /label=BBa_B0015
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagtc acacaggaaa gtactagatg aaaaacataa atgccgacga
     1261 cacatacaga ataattaata aaattaaagc ttgtagaagc aataatgata ttaatcaatg
     1321 cttatctgat atgactaaaa tggtacattg tgaatattat ttactcgcga tcatttatcc
     1381 tcattctatg gttaaatctg atatttcaat cctagataat taccctaaaa aatggaggca
     1441 atattatgat gacgctaatt taataaaata tgatcctata gtagattatt ctaactccaa
     1501 tcattcacca attaattgga atatatttga aaacaatgct gtaaataaaa aatctccaaa
     1561 tgtaattaaa gaagcgaaaa catcaggtct tatcactggg tttagtttcc ctattcatac
     1621 ggctaacaat ggcttcggaa tgcttagttt tgcacattca gaaaaagaca actatataga
     1681 tagtttattt ttacatgcgt gtatgaacat accattaatt gttccttctc tagttgataa
     1741 ttatcgaaaa ataaatatag caaataataa atcaaacaac gatttaacca aaagagaaaa
     1801 agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg gatatttcaa aaatattagg
     1861 ttgcagtgag cgtactgtca ctttccattt aaccaatgcg caaatgaaac tcaatacaac
     1921 aaaccgctgc caaagtattt ctaaagcaat tttaacagga gcaattgatt gcccatactt
     1981 taaaaattaa taacactgat agtgctagtg tagatcacta ctagagtcac acaggaaagt
     2041 actagatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg
     2101 aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg
     2161 aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg
     2221 acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca
     2281 tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact
     2341 tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca
     2401 tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa
     2461 aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag
     2521 gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa
     2581 ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac
     2641 tggacatcac ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag
     2701 gtcgtcactc caccggtgct taataacgct gatagtgcta gtgtagatcg ctactagagc
     2761 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     2821 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     2881 cgtttata
//