##gff-version 3
##sequence-region BBa_J22066 1 2888
BBa_J22066	.	promoter	1	1210	.	+	0	ID=BBa_I0500;Name=BBa_I0500
BBa_J22066	.	sequence_feature	1	1079	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_J22066	.	promoter	1080	1210	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_J22066	.	promoter	1043	1114	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_J22066	.	ribosome_entry_site	2027	2039	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_J22066	.	ribosome_entry_site	2027	2039	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J22066	.	engineered_region	2027	2039	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J22066	.	ribosome_entry_site	2033	2036	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J22066	.	CDS	1238	1993	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_J22066	.	CDS	1238	1987	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_J22066	.	engineered_region	1238	1239	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_J22066	.	point_mutation	1729	1729	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_J22066	.	engineered_tag	1994	2018	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_J22066	.	engineered_region	1238	1993	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_J22066	.	point_mutation	1411	1411	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_J22066	.	terminator	2760	2888	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J22066	.	terminator	2848	2888	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J22066	.	engineered_region	2848	2888	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J22066	.	stop_codon	2881	2881	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J22066	.	polyA_site	2875	2888	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J22066	.	stem_loop	2855	2874	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J22066	.	terminator	2760	2839	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J22066	.	stem_loop	2771	2814	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J22066	.	engineered_region	2760	2839	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J22066	.	CDS	2046	2751	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_J22066	.	CDS	2046	2720	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_J22066	.	engineered_tag	2727	2751	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_J22066	.	ribosome_entry_site	1219	1231	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_J22066	.	ribosome_entry_site	1219	1231	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J22066	.	engineered_region	1219	1231	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J22066	.	ribosome_entry_site	1225	1228	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
>BBa_J22066
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcat
tttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtgg
tgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa
aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga
tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc
gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg
cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccag
ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac
cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcac
caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa
ccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcg
cttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg
cgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa
gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac
tttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttc
tccatacccgtttttttgggctagctactagagtcacacaggaaagtactagatgaaaaacataaatgccgacgacaca
tacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatgg
tacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataatta
ccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaat
cattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaa
catcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattc
agaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgat
aattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtggg
catgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaa
tgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgccca
tactttaaaaattaataacactgatagtgctagtgtagatcactactagagtcacacaggaaagtactagatggcttcc
tccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcg
aaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgtt
cgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactac
ctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttaccc
aggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggt
tatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatc
aaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttc
agctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagta
cgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttata