LOCUS       BBa_J22071              1032 bp    DNA     linear       11-May-2024
DEFINITION  pTet aiia(+LVA)
ACCESSION   BBa_J22071
VERSION     BBa_J22071.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..75
                     /label=RBS-3\Weak
     RBS             69..72
                     /label=RBS
     misc_feature    63..75
                     /label=BBa_B0032
     RBS             63..75
                     /label=BBa_B0032
     misc_feature    82..84
                     /label=start
     misc_feature    865..870
                     /label=2
     misc_feature    832..864
                     /label=LVA
     CDS             82..831
                     /label=aiiA
     misc_feature    82..870
                     /label=BBa_C0060
     misc_feature    871..895
                     /label=Help:Barcodes
     CDS             82..870
                     /label=BBa_C0060
     stem_loop       915..958
                     /label=stem_loop
     misc_feature    904..983
                     /label=BBa_B0010
     terminator      904..983
                     /label=BBa_B0010
     stem_loop       999..1018
                     /label=T7 TE
     misc_feature    1025..1025
                     /label=stop
     polyA_site      1019..1032
                     /label=polya
     misc_feature    992..1032
                     /label=BBa_B0012
     terminator      992..1032
                     /label=BBa_B0012
     terminator      904..1032
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gaaagtacta gatgacagta aagaagcttt atttcgtccc agcaggtcgt
      121 tgtatgttgg atcattcgtc tgttaatagt acattaacac caggagaatt attagactta
      181 ccggtttggt gttatctttt ggagactgaa gaaggaccta ttttagtaga tacaggtatg
      241 ccagaaagtg cagttaataa tgaaggtctt tttaacggta catttgtcga agggcaggtt
      301 ttaccgaaaa tgactgaaga agatagaatc gtgaatattt taaaacgggt tggttatgag
      361 ccggaagacc ttctttatat tattagttct cacttgcatt ttgatcatgc aggaggaaat
      421 ggcgctttta taaatacacc aatcattgta cagcgtgctg aatatgaggc ggcgcagcat
      481 agcgaagaat atttgaaaga atgtatattg ccgaatttaa actacaaaat cattgaaggt
      541 gattatgaag tcgtaccagg agttcaatta ttgcatacac caggccatac tccagggcat
      601 caatcgctat taattgagac agaaaaatcc ggtcctgtat tattaacgat tgatgcatcg
      661 tatacgaaag agaattttga aaatgaagtg ccatttgcgg gatttgattc agaattagct
      721 ttatcttcaa ttaaacgttt aaaagaagtg gtgatgaaag agaagccgat tgttttcttt
      781 ggacatgata tagagcagga aaggggatgt aaagtgttcc ctgaatatat agctgcaaac
      841 gacgaaaact acgctttagt agcttaataa cgctgatagt gctagtgtag atcgctacta
      901 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
      961 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     1021 tctgcgttta ta
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