##gff-version 3
##sequence-region BBa_J22081 1 1044
BBa_J22081	.	engineered_region	146	907	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_J22081	.	stop_codon	902	907	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_J22081	.	tag	863	901	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_J22081	.	engineered_region	146	907	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_J22081	.	sequence_alteration	387	387	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_J22081	.	CDS	146	907	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_J22081	.	terminator	916	995	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J22081	.	engineered_region	916	995	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J22081	.	stem_loop	927	970	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J22081	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_J22081	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J22081	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J22081	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J22081	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J22081	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J22081	.	promoter	64	118	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_J22081	.	start_codon	116	116	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J22081	.	promoter	105	110	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J22081	.	promoter	83	88	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J22081	.	engineered_region	64	118	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J22081	.	non_covalent_binding_site	64	83	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J22081	.	terminator	1004	1044	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J22081	.	stop_codon	1037	1037	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J22081	.	polyA_site	1031	1044	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J22081	.	stem_loop	1011	1030	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J22081	.	engineered_region	1004	1044	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J22081	.	ribosome_entry_site	127	139	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_J22081	.	ribosome_entry_site	127	139	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J22081	.	engineered_region	127	139	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J22081	.	ribosome_entry_site	133	136	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
>BBa_J22081
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagacctgtaggatcgtac
aggtttacgcaagaaaatggtttgttatagtcgaataaatactagagtcacacaggaaagtactagatggtgagcaagg
gcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtc
cggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccc
tggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgact
tcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccg
cgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaac
atcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatca
aggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccat
cggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaag
cgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctg
ctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcga
aagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgg
gcctttctgcgtttata