LOCUS BBa_J22091 3031 bp DNA linear 17-May-2024 DEFINITION J22090 J22081 J22061 ACCESSION BBa_J22091 VERSION BBa_J22091.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 64..83 /label=LuxR/HSL promoter 83..88 /label=-35 promoter 105..110 /label=-10 misc_feature 116..116 /label=start misc_feature 64..118 /label=BBa_R0062 promoter 64..118 /label=BBa_R0062 promoter 127..251 /label=BBa_J22086 stem_loop 271..314 /label=stem_loop misc_feature 260..339 /label=BBa_B0010 terminator 260..339 /label=BBa_B0010 stem_loop 355..374 /label=T7 TE misc_feature 381..381 /label=stop polyA_site 375..388 /label=polya misc_feature 348..388 /label=BBa_B0012 terminator 348..388 /label=BBa_B0012 misc_feature 1..388 /label=BBa_J22090 misc_feature 399..415 /label=lac O1 promoter 416..421 /label=-35 misc_feature 422..438 /label=lac O1 promoter 439..444 /label=-10 misc_feature 397..450 /label=BBa_R0011 promoter 397..450 /label=BBa_R0011 misc_feature 460..479 /label=LuxR/HSL promoter 479..484 /label=-35 promoter 501..506 /label=-10 misc_feature 512..512 /label=start misc_feature 460..514 /label=BBa_R0062 promoter 460..514 /label=BBa_R0062 RBS 523..535 /label=RBS-3\Weak RBS 529..532 /label=RBS misc_feature 523..535 /label=BBa_B0032 RBS 523..535 /label=BBa_B0032 CDS 542..1303 /label=YFP (LVA) misc_feature 1298..1303 /label=2 misc_feature 1259..1297 /label=SsrA misc_feature 783..783 /label=A (Q->L) misc_feature 542..1303 /label=BBa_E0032 misc_feature 542..1303 /label=BBa_E0032 stem_loop 1323..1366 /label=stem_loop misc_feature 1312..1391 /label=BBa_B0010 terminator 1312..1391 /label=BBa_B0010 stem_loop 1407..1426 /label=T7 TE misc_feature 1433..1433 /label=stop polyA_site 1427..1440 /label=polya misc_feature 1400..1440 /label=BBa_B0012 terminator 1400..1440 /label=BBa_B0012 misc_feature 397..1440 /label=BBa_J22081 promoter 1449..1513 /label=BBa_J22052 misc_feature 1528..1531 /label=conserved RBS 1522..1535 /label=BBa_B0031 CDS 1542..3031 /label=BBa_J22056 misc_feature 1449..3031 /label=BBa_J22061 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagacctgta ggatcgtaca ggtttacgca agaaaatggt ttgttatagt cgaataaata 121 ctagagtcga gtctcgtttc ccgctccaaa ttcttctctc aataaaatat ccacagcgac 181 gcgatgcgtt attgctggtt tttgttgtct ctgacaaact cttgtaaaca gagttatcca 241 cagcctcagg ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 301 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 361 ccttcgggtg ggcctttctg cgtttatata ctagagaatt gtgagcggat aacaattgac 421 attgtgagcg gataacaaga tactgagcac atactagaga cctgtaggat cgtacaggtt 481 tacgcaagaa aatggtttgt tatagtcgaa taaatactag agtcacacag gaaagtacta 541 gatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga 601 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta 661 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac 721 cctcgtgacc accttcggct acggcctgca atgcttcgcc cgctaccccg accacatgaa 781 gctgcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt 841 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct 901 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca 961 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa 1021 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc 1081 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca 1141 ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt 1201 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagag 1261 gcctgctgca aacgacgaaa actacgcttt agtagcttaa taatactaga gccaggcatc 1321 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 1381 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 1441 tactagaggt atgtagcgca tctttcttta cggtcaatca gcaaggtgtt aaattgatca 1501 cgttttagac cattactaga gtcacacagg aaacctacta gatgactata atgataaaaa 1561 aatcggattt tttggcaatt ccatcggagg agtataaagg tattctaagt cttcgttatc 1621 aagtgtttaa gcaaagactt gagtgggact tagttgtaga aaataacctt gaatcagatg 1681 agtatgataa ctcaaatgca gaatatattt atgcttgtga tgatactgaa aatgtaagtg 1741 gatgctggcg tttattacct acaacaggtg attatatgct gaaaagtgtt tttcctgaat 1801 tgcttggtca acagagtgct cccaaagatc ctaatatagt cgaattaagt cgttttgctg 1861 taggtaaaaa tagctcaaag ataaataact ctgctagtga aattacaatg aaactatttg 1921 aagctatata taaacacgct gttagtcaag gtattacaga atatgtaaca gtaacatcaa 1981 cagcaataga gcgattttta aagcgtatta aagttccttg tcatcgtatt ggagacaaag 2041 aaattcatgt attaggtgat actaaatcgg ttgtattgtc tatgcctatt aatgaacagt 2101 ttaaaaaagc agtcttaaat ggtggtggtt caatggtgag caagggcgag gagctgttca 2161 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt gaacggccac aagttcagcg 2221 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca 2281 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tggggcgtgc 2341 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc 2401 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc 2461 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg 2521 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac atcagccaca 2581 acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc 2641 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg 2701 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca 2761 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga 2821 tcactctcgg catggacgag ctgtacaaga ggcctgctgc aaacgacgaa aactacgctt 2881 tagtagctta ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac 2941 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc 3001 tcaccttcgg gtgggccttt ctgcgtttat a //