##gff-version 3
##sequence-region BBa_J22091 1 3031
BBa_J22091	.	engineered_region	397	1440	.	+	0	ID=BBa_J22081;Name=BBa_J22081
BBa_J22091	.	terminator	1400	1440	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J22081
BBa_J22091	.	stem_loop	1407	1426	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J22091	.	polyA_site	1427	1440	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J22091	.	engineered_region	1400	1440	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J22091	.	stop_codon	1433	1433	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J22091	.	engineered_region	542	1303	.	+	0	ID=BBa_E0032;Name=BBa_E0032;Parent=BBa_J22081
BBa_J22091	.	stop_codon	1298	1303	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_J22091	.	sequence_alteration	783	783	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_J22091	.	engineered_region	542	1303	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_J22091	.	tag	1259	1297	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_J22091	.	CDS	542	1303	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_J22091	.	terminator	1312	1391	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J22081
BBa_J22091	.	stem_loop	1323	1366	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J22091	.	engineered_region	1312	1391	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J22091	.	promoter	397	450	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_J22081
BBa_J22091	.	operator	422	438	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J22091	.	promoter	439	444	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J22091	.	promoter	416	421	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J22091	.	engineered_region	397	450	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J22091	.	operator	399	415	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J22091	.	promoter	460	514	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_J22081
BBa_J22091	.	promoter	479	484	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J22091	.	non_covalent_binding_site	460	479	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J22091	.	promoter	501	506	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J22091	.	start_codon	512	512	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J22091	.	engineered_region	460	514	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J22091	.	ribosome_entry_site	523	535	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_J22081
BBa_J22091	.	ribosome_entry_site	529	532	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J22091	.	ribosome_entry_site	523	535	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J22091	.	engineered_region	523	535	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J22091	.	engineered_region	1449	3031	.	+	0	ID=BBa_J22061;Name=BBa_J22061
BBa_J22091	.	CDS	1542	3031	.	+	0	ID=BBa_J22056;Name=BBa_J22056;Parent=BBa_J22061
BBa_J22091	.	promoter	1449	1513	.	+	0	ID=BBa_J22052;Name=BBa_J22052;Parent=BBa_J22061
BBa_J22091	.	ribosome_entry_site	1522	1535	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_J22061
BBa_J22091	.	conserved_region	1528	1531	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_J22091	.	engineered_region	1	388	.	+	0	ID=BBa_J22090;Name=BBa_J22090
BBa_J22091	.	promoter	64	118	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_J22090
BBa_J22091	.	promoter	83	88	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J22091	.	non_covalent_binding_site	64	83	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J22091	.	promoter	105	110	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J22091	.	start_codon	116	116	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J22091	.	engineered_region	64	118	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J22091	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_J22090
BBa_J22091	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J22091	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J22091	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J22091	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J22091	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J22091	.	terminator	260	339	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J22090
BBa_J22091	.	stem_loop	271	314	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J22091	.	engineered_region	260	339	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J22091	.	promoter	127	251	.	+	0	ID=BBa_J22086;Name=BBa_J22086;Parent=BBa_J22090
BBa_J22091	.	terminator	348	388	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J22090
BBa_J22091	.	stem_loop	355	374	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J22091	.	polyA_site	375	388	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J22091	.	engineered_region	348	388	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J22091	.	stop_codon	381	381	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
>BBa_J22091
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagacctgtaggatcgtac
aggtttacgcaagaaaatggtttgttatagtcgaataaatactagagtcgagtctcgtttcccgctccaaattcttctc
tcaataaaatatccacagcgacgcgatgcgttattgctggtttttgttgtctctgacaaactcttgtaaacagagttat
ccacagcctcaggctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatct
gttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactaga
gaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagacctgtaggatcgta
caggtttacgcaagaaaatggtttgttatagtcgaataaatactagagtcacacaggaaagtactagatggtgagcaag
ggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgt
ccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgcc
ctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgac
ttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagaccc
gcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaa
catcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatc
aaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacaccccca
tcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaa
gcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcct
gctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcg
aaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtg
ggcctttctgcgtttatatactagaggtatgtagcgcatctttctttacggtcaatcagcaaggtgttaaattgatcac
gttttagaccattactagagtcacacaggaaacctactagatgactataatgataaaaaaatcggattttttggcaatt
ccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtag
aaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaag
tggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagt
gctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgcta
gtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaac
atcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtatta
ggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatggtggtggttcaa
tggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaa
gttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaag
ctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatga
agcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaa
ctacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaag
gaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcaga
agaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagca
gaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagac
cccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgt
acaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttata