LOCUS BBa_J22092 6571 bp DNA linear 23-Dec-2024 DEFINITION cell cycle oscilator+ LuxI-LuxR module ACCESSION BBa_J22092 VERSION BBa_J22092.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 64..83 /label=LuxR/HSL promoter 83..88 /label=-35 promoter 105..110 /label=-10 misc_feature 116..116 /label=start misc_feature 64..118 /label=BBa_R0062 promoter 64..118 /label=BBa_R0062 RBS 127..139 /label=RBS-3\Weak RBS 133..136 /label=RBS misc_feature 127..139 /label=BBa_B0032 RBS 127..139 /label=BBa_B0032 CDS 146..907 /label=YFP (LVA) misc_feature 902..907 /label=2 misc_feature 863..901 /label=SsrA misc_feature 387..387 /label=A (Q->L) misc_feature 146..907 /label=BBa_E0032 misc_feature 146..907 /label=BBa_E0032 stem_loop 927..970 /label=stem_loop misc_feature 916..995 /label=BBa_B0010 terminator 916..995 /label=BBa_B0010 stem_loop 1011..1030 /label=T7 TE misc_feature 1037..1037 /label=stop polyA_site 1031..1044 /label=polya misc_feature 1004..1044 /label=BBa_B0012 terminator 1004..1044 /label=BBa_B0012 misc_feature 1..1044 /label=BBa_J22081 promoter 1053..1117 /label=BBa_J22052 misc_feature 1132..1135 /label=conserved RBS 1126..1139 /label=BBa_B0031 CDS 1146..2635 /label=BBa_J22056 misc_feature 1053..2635 /label=BBa_J22061 promoter 3723..3853 /label=Pbad promoter 3686..3757 /label=PC misc_feature 2644..3722 /label=AraC promoter 2644..3853 /label=BBa_I0500 RBS 3862..3874 /label=RBS-3\Weak RBS 3868..3871 /label=RBS misc_feature 3862..3874 /label=BBa_B0032 RBS 3862..3874 /label=BBa_B0032 misc_feature 3881..3882 /label=prefix misc_feature 4054..4054 /label=T misc_feature 4372..4372 /label=A CDS 3881..4630 /label=luxR misc_feature 3881..4636 /label=BBa_C0062 misc_feature 4637..4661 /label=Help:Barcodes CDS 3881..4636 /label=BBa_C0062 RBS 4670..4682 /label=RBS-3\Weak RBS 4676..4679 /label=RBS misc_feature 4670..4682 /label=BBa_B0032 RBS 4670..4682 /label=BBa_B0032 CDS 4689..5363 /label=mrfp1 misc_feature 5370..5394 /label=Help:Barcodes CDS 4689..5394 /label=BBa_E1010 stem_loop 5414..5457 /label=stem_loop misc_feature 5403..5482 /label=BBa_B0010 terminator 5403..5482 /label=BBa_B0010 stem_loop 5498..5517 /label=T7 TE misc_feature 5524..5524 /label=stop polyA_site 5518..5531 /label=polya misc_feature 5491..5531 /label=BBa_B0012 terminator 5491..5531 /label=BBa_B0012 terminator 5403..5531 /label=BBa_B0015 misc_feature 2644..5531 /label=BBa_J22066 misc_feature 5540..5558 /label=TetR 1 misc_feature 5540..5593 /label=BBa_R0040 promoter 5559..5564 /label=-35 misc_feature 5565..5583 /label=TetR 2 promoter 5582..5587 /label=-10 promoter 5540..5593 /label=BBa_R0040 RBS 5602..5614 /label=RBS-3\Weak RBS 5608..5611 /label=RBS misc_feature 5602..5614 /label=BBa_B0032 RBS 5602..5614 /label=BBa_B0032 misc_feature 5621..5623 /label=start misc_feature 6404..6409 /label=2 misc_feature 6371..6403 /label=LVA CDS 5621..6370 /label=aiiA misc_feature 5621..6409 /label=BBa_C0060 misc_feature 6410..6434 /label=Help:Barcodes CDS 5621..6409 /label=BBa_C0060 stem_loop 6454..6497 /label=stem_loop misc_feature 6443..6522 /label=BBa_B0010 terminator 6443..6522 /label=BBa_B0010 stem_loop 6538..6557 /label=T7 TE misc_feature 6564..6564 /label=stop polyA_site 6558..6571 /label=polya misc_feature 6531..6571 /label=BBa_B0012 terminator 6531..6571 /label=BBa_B0012 terminator 6443..6571 /label=BBa_B0015 misc_feature 5540..6571 /label=BBa_J22071 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagacctgta ggatcgtaca ggtttacgca agaaaatggt ttgttatagt cgaataaata 121 ctagagtcac acaggaaagt actagatggt gagcaagggc gaggagctgt tcaccggggt 181 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg 241 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg 301 caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcaatgctt 361 cgcccgctac cccgaccaca tgaagctgca cgacttcttc aagtccgcca tgcccgaagg 421 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga 481 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa 541 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta 601 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat 661 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg 721 ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc 781 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct 841 cggcatggac gagctgtaca agaggcctgc tgcaaacgac gaaaactacg ctttagtagc 901 ttaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 961 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt 1021 cgggtgggcc tttctgcgtt tatatactag aggtatgtag cgcatctttc tttacggtca 1081 atcagcaagg tgttaaattg atcacgtttt agaccattac tagagtcaca caggaaacct 1141 actagatgac tataatgata aaaaaatcgg attttttggc aattccatcg gaggagtata 1201 aaggtattct aagtcttcgt tatcaagtgt ttaagcaaag acttgagtgg gacttagttg 1261 tagaaaataa ccttgaatca gatgagtatg ataactcaaa tgcagaatat atttatgctt 1321 gtgatgatac tgaaaatgta agtggatgct ggcgtttatt acctacaaca ggtgattata 1381 tgctgaaaag tgtttttcct gaattgcttg gtcaacagag tgctcccaaa gatcctaata 1441 tagtcgaatt aagtcgtttt gctgtaggta aaaatagctc aaagataaat aactctgcta 1501 gtgaaattac aatgaaacta tttgaagcta tatataaaca cgctgttagt caaggtatta 1561 cagaatatgt aacagtaaca tcaacagcaa tagagcgatt tttaaagcgt attaaagttc 1621 cttgtcatcg tattggagac aaagaaattc atgtattagg tgatactaaa tcggttgtat 1681 tgtctatgcc tattaatgaa cagtttaaaa aagcagtctt aaatggtggt ggttcaatgg 1741 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg 1801 acgtgaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca 1861 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg 1921 tgaccaccct gacctggggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc 1981 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca 2041 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga 2101 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc 2161 tggagtacaa ctacatcagc cacaacgtct atatcaccgc cgacaagcag aagaacggca 2221 tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc 2281 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc 2341 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc 2401 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagaggcctg 2461 ctgcaaacga cgaaaactac gctttagtag cttaataata ctagagccag gcatcaaata 2521 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 2581 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta 2641 gagttatgac aacttgacgg ctacatcatt cactttttct tcacaaccgg cacggaactc 2701 gctcgggctg gccccggtgc attttttaaa tacccgcgag aaatagagtt gatcgtcaaa 2761 accaacattg cgaccgacgg tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc 2821 ctggctgata cgttggtcct cgcgccagct taagacgcta atccctaact gctggcggaa 2881 aagatgtgac agacgcgacg gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa 2941 attgctgtct gccaggtgat cgctgatgta ctgacaagcc tcgcgtaccc gattatccat 3001 cggtggatgg agcgactcgt taatcgcttc catgcgccgc agtaacaatt gctcaagcag 3061 atttatcgcc agcagctccg aatagcgccc ttccccttgc ccggcgttaa tgatttgccc 3121 aaacaggtcg ctgaaatgcg gctggtgcgc ttcatccggg cgaaagaacc ccgtattggc 3181 aaatattgac ggccagttaa gccattcatg ccagtaggcg cgcggacgaa agtaaaccca 3241 ctggtgatac cattcgcgag cctccggatg acgaccgtag tgatgaatct ctcctggcgg 3301 gaacagcaaa atatcacccg gtcggcaaac aaattctcgt ccctgatttt tcaccacccc 3361 ctgaccgcga atggtgagat tgagaatata acctttcatt cccagcggtc ggtcgataaa 3421 aaaatcgaga taaccgttgg cctcaatcgg cgttaaaccc gccaccagat gggcattaaa 3481 cgagtatccc ggcagcaggg gatcattttg cgcttcagcc atacttttca tactcccgcc 3541 attcagagaa gaaaccaatt gtccatattg catcagacat tgccgtcact gcgtctttta 3601 ctggctcttc tcgctaacca aaccggtaac cccgcttatt aaaagcattc tgtaacaaag 3661 cgggaccaaa gccatgacaa aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt 3721 ccacattgat tatttgcacg gcgtcacact ttgctatgcc atagcatttt tatccataag 3781 attagcggat cctacctgac gctttttatc gcaactctct actgtttctc catacccgtt 3841 tttttgggct agctactaga gtcacacagg aaagtactag atgaaaaaca taaatgccga 3901 cgacacatac agaataatta ataaaattaa agcttgtaga agcaataatg atattaatca 3961 atgcttatct gatatgacta aaatggtaca ttgtgaatat tatttactcg cgatcattta 4021 tcctcattct atggttaaat ctgatatttc aatcctagat aattacccta aaaaatggag 4081 gcaatattat gatgacgcta atttaataaa atatgatcct atagtagatt attctaactc 4141 caatcattca ccaattaatt ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc 4201 aaatgtaatt aaagaagcga aaacatcagg tcttatcact gggtttagtt tccctattca 4261 tacggctaac aatggcttcg gaatgcttag ttttgcacat tcagaaaaag acaactatat 4321 agatagttta tttttacatg cgtgtatgaa cataccatta attgttcctt ctctagttga 4381 taattatcga aaaataaata tagcaaataa taaatcaaac aacgatttaa ccaaaagaga 4441 aaaagaatgt ttagcgtggg catgcgaagg aaaaagctct tgggatattt caaaaatatt 4501 aggttgcagt gagcgtactg tcactttcca tttaaccaat gcgcaaatga aactcaatac 4561 aacaaaccgc tgccaaagta tttctaaagc aattttaaca ggagcaattg attgcccata 4621 ctttaaaaat taataacact gatagtgcta gtgtagatca ctactagagt cacacaggaa 4681 agtactagat ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc aaagttcgta 4741 tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt 4801 acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt 4861 gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa cacccggctg 4921 acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa cgtgttatga 4981 acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa gacggtgagt 5041 tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg gttatgcaga 5101 aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac ggtgctctga 5161 aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac gctgaagtta 5221 aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa accgacatca 5281 aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac gaacgtgctg 5341 aaggtcgtca ctccaccggt gcttaataac gctgatagtg ctagtgtaga tcgctactag 5401 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 5461 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 5521 ctgcgtttat atactagagt ccctatcagt gatagagatt gacatcccta tcagtgatag 5581 agatactgag cactactaga gtcacacagg aaagtactag atgacagtaa agaagcttta 5641 tttcgtccca gcaggtcgtt gtatgttgga tcattcgtct gttaatagta cattaacacc 5701 aggagaatta ttagacttac cggtttggtg ttatcttttg gagactgaag aaggacctat 5761 tttagtagat acaggtatgc cagaaagtgc agttaataat gaaggtcttt ttaacggtac 5821 atttgtcgaa gggcaggttt taccgaaaat gactgaagaa gatagaatcg tgaatatttt 5881 aaaacgggtt ggttatgagc cggaagacct tctttatatt attagttctc acttgcattt 5941 tgatcatgca ggaggaaatg gcgcttttat aaatacacca atcattgtac agcgtgctga 6001 atatgaggcg gcgcagcata gcgaagaata tttgaaagaa tgtatattgc cgaatttaaa 6061 ctacaaaatc attgaaggtg attatgaagt cgtaccagga gttcaattat tgcatacacc 6121 aggccatact ccagggcatc aatcgctatt aattgagaca gaaaaatccg gtcctgtatt 6181 attaacgatt gatgcatcgt atacgaaaga gaattttgaa aatgaagtgc catttgcggg 6241 atttgattca gaattagctt tatcttcaat taaacgttta aaagaagtgg tgatgaaaga 6301 gaagccgatt gttttctttg gacatgatat agagcaggaa aggggatgta aagtgttccc 6361 tgaatatata gctgcaaacg acgaaaacta cgctttagta gcttaataac gctgatagtg 6421 ctagtgtaga tcgctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac 6481 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc 6541 tcaccttcgg gtgggccttt ctgcgtttat a //