##gff-version 3
##sequence-region BBa_J22092 1 6571
BBa_J22092	.	engineered_region	1	1044	.	+	0	ID=BBa_J22081;Name=BBa_J22081
BBa_J22092	.	terminator	916	995	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J22081
BBa_J22092	.	stem_loop	927	970	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J22092	.	engineered_region	916	995	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J22092	.	engineered_region	146	907	.	+	0	ID=BBa_E0032;Name=BBa_E0032;Parent=BBa_J22081
BBa_J22092	.	stop_codon	902	907	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_J22092	.	CDS	146	907	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_J22092	.	sequence_alteration	387	387	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_J22092	.	engineered_region	146	907	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_J22092	.	tag	863	901	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_J22092	.	promoter	64	118	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_J22081
BBa_J22092	.	engineered_region	64	118	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J22092	.	promoter	83	88	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J22092	.	promoter	105	110	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J22092	.	start_codon	116	116	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J22092	.	non_covalent_binding_site	64	83	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J22092	.	terminator	1004	1044	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J22081
BBa_J22092	.	polyA_site	1031	1044	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J22092	.	engineered_region	1004	1044	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J22092	.	stem_loop	1011	1030	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J22092	.	stop_codon	1037	1037	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J22092	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_J22081
BBa_J22092	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J22092	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J22092	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J22092	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J22092	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J22092	.	ribosome_entry_site	127	139	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_J22081
BBa_J22092	.	engineered_region	127	139	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J22092	.	ribosome_entry_site	127	139	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J22092	.	ribosome_entry_site	133	136	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J22092	.	engineered_region	2644	5531	.	+	0	ID=BBa_J22066;Name=BBa_J22066
BBa_J22092	.	promoter	2644	3853	.	+	0	ID=BBa_I0500;Name=BBa_I0500;Parent=BBa_J22066
BBa_J22092	.	promoter	3686	3757	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_J22092	.	promoter	3723	3853	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_J22092	.	sequence_feature	2644	3722	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_J22092	.	ribosome_entry_site	4670	4682	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_J22066
BBa_J22092	.	engineered_region	4670	4682	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J22092	.	ribosome_entry_site	4670	4682	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J22092	.	ribosome_entry_site	4676	4679	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J22092	.	CDS	3881	4636	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_J22066
BBa_J22092	.	CDS	3881	4630	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_J22092	.	engineered_tag	4637	4661	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_J22092	.	engineered_region	3881	4636	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_J22092	.	point_mutation	4054	4054	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_J22092	.	engineered_region	3881	3882	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_J22092	.	point_mutation	4372	4372	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_J22092	.	ribosome_entry_site	3862	3874	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_J22066
BBa_J22092	.	engineered_region	3862	3874	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J22092	.	ribosome_entry_site	3862	3874	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J22092	.	ribosome_entry_site	3868	3871	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J22092	.	CDS	4689	5394	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J22066
BBa_J22092	.	CDS	4689	5363	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_J22092	.	engineered_tag	5370	5394	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_J22092	.	terminator	5403	5531	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_J22066
BBa_J22092	.	terminator	5491	5531	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J22092	.	polyA_site	5518	5531	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J22092	.	engineered_region	5491	5531	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J22092	.	stem_loop	5498	5517	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J22092	.	stop_codon	5524	5524	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J22092	.	terminator	5403	5482	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J22092	.	stem_loop	5414	5457	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J22092	.	engineered_region	5403	5482	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J22092	.	engineered_region	5540	6571	.	+	0	ID=BBa_J22071;Name=BBa_J22071
BBa_J22092	.	CDS	5621	6409	.	+	0	ID=BBa_C0060;Name=BBa_C0060;Parent=BBa_J22071
BBa_J22092	.	engineered_tag	6410	6434	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_J22092	.	tag	6371	6403	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_J22092	.	start_codon	5621	5623	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_J22092	.	stop_codon	6404	6409	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_J22092	.	CDS	5621	6370	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_J22092	.	engineered_region	5621	6409	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_J22092	.	promoter	5540	5593	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J22071
BBa_J22092	.	engineered_region	5540	5593	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J22092	.	non_covalent_binding_site	5540	5558	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J22092	.	non_covalent_binding_site	5565	5583	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J22092	.	promoter	5559	5564	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J22092	.	promoter	5582	5587	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J22092	.	terminator	6443	6571	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_J22071
BBa_J22092	.	terminator	6531	6571	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J22092	.	polyA_site	6558	6571	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J22092	.	engineered_region	6531	6571	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J22092	.	stem_loop	6538	6557	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J22092	.	stop_codon	6564	6564	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J22092	.	terminator	6443	6522	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J22092	.	stem_loop	6454	6497	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J22092	.	engineered_region	6443	6522	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J22092	.	ribosome_entry_site	5602	5614	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_J22071
BBa_J22092	.	engineered_region	5602	5614	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J22092	.	ribosome_entry_site	5602	5614	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J22092	.	ribosome_entry_site	5608	5611	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J22092	.	engineered_region	1053	2635	.	+	0	ID=BBa_J22061;Name=BBa_J22061
BBa_J22092	.	CDS	1146	2635	.	+	0	ID=BBa_J22056;Name=BBa_J22056;Parent=BBa_J22061
BBa_J22092	.	ribosome_entry_site	1126	1139	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_J22061
BBa_J22092	.	conserved_region	1132	1135	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_J22092	.	promoter	1053	1117	.	+	0	ID=BBa_J22052;Name=BBa_J22052;Parent=BBa_J22061
>BBa_J22092
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagacctgtaggatcgtac
aggtttacgcaagaaaatggtttgttatagtcgaataaatactagagtcacacaggaaagtactagatggtgagcaagg
gcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtc
cggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccc
tggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgact
tcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccg
cgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaac
atcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatca
aggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccat
cggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaag
cgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctg
ctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcga
aagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgg
gcctttctgcgtttatatactagaggtatgtagcgcatctttctttacggtcaatcagcaaggtgttaaattgatcacg
ttttagaccattactagagtcacacaggaaacctactagatgactataatgataaaaaaatcggattttttggcaattc
catcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtaga
aaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagt
ggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtg
ctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctag
tgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaaca
tcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattag
gtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatggtggtggttcaat
ggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaag
ttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagc
tgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaa
gcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaac
tacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaagg
aggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaa
gaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcag
aacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagacc
ccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgta
caagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaa
ggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctca
ccttcgggtgggcctttctgcgtttatatactagagttatgacaacttgacggctacatcattcactttttcttcacaa
ccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaa
cattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcg
ccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcg
acgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcg
gtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccga
atagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccggg
cgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaaccc
actggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacc
cggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttc
attcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcat
taaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaac
caattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccg
cttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggca
gaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcc
tacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagtcacacagga
aagtactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatg
atattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattc
tatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaata
aaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaa
ataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggc
taacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgt
atgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacg
atttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattagg
ttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatt
tctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
tactagagtcacacaggaaagtactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgta
tggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgc
taaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaa
gcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgtta
tgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaa
actgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgt
atgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctg
aagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcac
ctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgc
tgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcg
ttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttat
atactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacag
gaaagtactagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatag
tacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagta
gatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccga
aaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattag
ttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatat
gaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgatt
atgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacaga
aaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcggga
tttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttg
gacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagt
agcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttata