LOCUS BBa_J22095 6967 bp DNA linear 12-May-2024 DEFINITION Synthetic coupling of cell cycle oscillators ACCESSION BBa_J22095 VERSION BBa_J22095.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 64..83 /label=LuxR/HSL promoter 83..88 /label=-35 promoter 105..110 /label=-10 misc_feature 116..116 /label=start misc_feature 64..118 /label=BBa_R0062 promoter 64..118 /label=BBa_R0062 promoter 127..251 /label=BBa_J22086 stem_loop 271..314 /label=stem_loop misc_feature 260..339 /label=BBa_B0010 terminator 260..339 /label=BBa_B0010 stem_loop 355..374 /label=T7 TE misc_feature 381..381 /label=stop polyA_site 375..388 /label=polya misc_feature 348..388 /label=BBa_B0012 terminator 348..388 /label=BBa_B0012 misc_feature 1..388 /label=BBa_J22090 misc_feature 399..415 /label=lac O1 promoter 416..421 /label=-35 misc_feature 422..438 /label=lac O1 promoter 439..444 /label=-10 misc_feature 397..450 /label=BBa_R0011 promoter 397..450 /label=BBa_R0011 misc_feature 460..479 /label=LuxR/HSL promoter 479..484 /label=-35 promoter 501..506 /label=-10 misc_feature 512..512 /label=start misc_feature 460..514 /label=BBa_R0062 promoter 460..514 /label=BBa_R0062 RBS 523..535 /label=RBS-3\Weak RBS 529..532 /label=RBS misc_feature 523..535 /label=BBa_B0032 RBS 523..535 /label=BBa_B0032 CDS 542..1303 /label=YFP (LVA) misc_feature 1298..1303 /label=2 misc_feature 1259..1297 /label=SsrA misc_feature 783..783 /label=A (Q->L) misc_feature 542..1303 /label=BBa_E0032 misc_feature 542..1303 /label=BBa_E0032 stem_loop 1323..1366 /label=stem_loop misc_feature 1312..1391 /label=BBa_B0010 terminator 1312..1391 /label=BBa_B0010 stem_loop 1407..1426 /label=T7 TE misc_feature 1433..1433 /label=stop polyA_site 1427..1440 /label=polya misc_feature 1400..1440 /label=BBa_B0012 terminator 1400..1440 /label=BBa_B0012 misc_feature 397..1440 /label=BBa_J22081 promoter 1449..1513 /label=BBa_J22052 misc_feature 1528..1531 /label=conserved RBS 1522..1535 /label=BBa_B0031 CDS 1542..3031 /label=BBa_J22056 misc_feature 1449..3031 /label=BBa_J22061 promoter 4119..4249 /label=Pbad promoter 4082..4153 /label=PC misc_feature 3040..4118 /label=AraC promoter 3040..4249 /label=BBa_I0500 RBS 4258..4270 /label=RBS-3\Weak RBS 4264..4267 /label=RBS misc_feature 4258..4270 /label=BBa_B0032 RBS 4258..4270 /label=BBa_B0032 misc_feature 4277..4278 /label=prefix misc_feature 4450..4450 /label=T misc_feature 4768..4768 /label=A CDS 4277..5026 /label=luxR misc_feature 4277..5032 /label=BBa_C0062 misc_feature 5033..5057 /label=Help:Barcodes CDS 4277..5032 /label=BBa_C0062 RBS 5066..5078 /label=RBS-3\Weak RBS 5072..5075 /label=RBS misc_feature 5066..5078 /label=BBa_B0032 RBS 5066..5078 /label=BBa_B0032 CDS 5085..5759 /label=mrfp1 misc_feature 5766..5790 /label=Help:Barcodes CDS 5085..5790 /label=BBa_E1010 stem_loop 5810..5853 /label=stem_loop misc_feature 5799..5878 /label=BBa_B0010 terminator 5799..5878 /label=BBa_B0010 stem_loop 5894..5913 /label=T7 TE misc_feature 5920..5920 /label=stop polyA_site 5914..5927 /label=polya misc_feature 5887..5927 /label=BBa_B0012 terminator 5887..5927 /label=BBa_B0012 terminator 5799..5927 /label=BBa_B0015 misc_feature 3040..5927 /label=BBa_J22066 misc_feature 5936..5954 /label=TetR 1 misc_feature 5936..5989 /label=BBa_R0040 promoter 5955..5960 /label=-35 misc_feature 5961..5979 /label=TetR 2 promoter 5978..5983 /label=-10 promoter 5936..5989 /label=BBa_R0040 RBS 5998..6010 /label=RBS-3\Weak RBS 6004..6007 /label=RBS misc_feature 5998..6010 /label=BBa_B0032 RBS 5998..6010 /label=BBa_B0032 misc_feature 6017..6019 /label=start misc_feature 6800..6805 /label=2 misc_feature 6767..6799 /label=LVA CDS 6017..6766 /label=aiiA misc_feature 6017..6805 /label=BBa_C0060 misc_feature 6806..6830 /label=Help:Barcodes CDS 6017..6805 /label=BBa_C0060 stem_loop 6850..6893 /label=stem_loop misc_feature 6839..6918 /label=BBa_B0010 terminator 6839..6918 /label=BBa_B0010 stem_loop 6934..6953 /label=T7 TE misc_feature 6960..6960 /label=stop polyA_site 6954..6967 /label=polya misc_feature 6927..6967 /label=BBa_B0012 terminator 6927..6967 /label=BBa_B0012 terminator 6839..6967 /label=BBa_B0015 misc_feature 5936..6967 /label=BBa_J22071 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagacctgta ggatcgtaca ggtttacgca agaaaatggt ttgttatagt cgaataaata 121 ctagagtcga gtctcgtttc ccgctccaaa ttcttctctc aataaaatat ccacagcgac 181 gcgatgcgtt attgctggtt tttgttgtct ctgacaaact cttgtaaaca gagttatcca 241 cagcctcagg ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 301 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 361 ccttcgggtg ggcctttctg cgtttatata ctagagaatt gtgagcggat aacaattgac 421 attgtgagcg gataacaaga tactgagcac atactagaga cctgtaggat cgtacaggtt 481 tacgcaagaa aatggtttgt tatagtcgaa taaatactag agtcacacag gaaagtacta 541 gatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga 601 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta 661 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac 721 cctcgtgacc accttcggct acggcctgca atgcttcgcc cgctaccccg accacatgaa 781 gctgcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt 841 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct 901 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca 961 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa 1021 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc 1081 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca 1141 ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt 1201 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagag 1261 gcctgctgca aacgacgaaa actacgcttt agtagcttaa taatactaga gccaggcatc 1321 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 1381 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 1441 tactagaggt atgtagcgca tctttcttta cggtcaatca gcaaggtgtt aaattgatca 1501 cgttttagac cattactaga gtcacacagg aaacctacta gatgactata atgataaaaa 1561 aatcggattt tttggcaatt ccatcggagg agtataaagg tattctaagt cttcgttatc 1621 aagtgtttaa gcaaagactt gagtgggact tagttgtaga aaataacctt gaatcagatg 1681 agtatgataa ctcaaatgca gaatatattt atgcttgtga tgatactgaa aatgtaagtg 1741 gatgctggcg tttattacct acaacaggtg attatatgct gaaaagtgtt tttcctgaat 1801 tgcttggtca acagagtgct cccaaagatc ctaatatagt cgaattaagt cgttttgctg 1861 taggtaaaaa tagctcaaag ataaataact ctgctagtga aattacaatg aaactatttg 1921 aagctatata taaacacgct gttagtcaag gtattacaga atatgtaaca gtaacatcaa 1981 cagcaataga gcgattttta aagcgtatta aagttccttg tcatcgtatt ggagacaaag 2041 aaattcatgt attaggtgat actaaatcgg ttgtattgtc tatgcctatt aatgaacagt 2101 ttaaaaaagc agtcttaaat ggtggtggtt caatggtgag caagggcgag gagctgttca 2161 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt gaacggccac aagttcagcg 2221 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca 2281 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tggggcgtgc 2341 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc 2401 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc 2461 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg 2521 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac atcagccaca 2581 acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc 2641 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg 2701 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca 2761 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga 2821 tcactctcgg catggacgag ctgtacaaga ggcctgctgc aaacgacgaa aactacgctt 2881 tagtagctta ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac 2941 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc 3001 tcaccttcgg gtgggccttt ctgcgtttat atactagagt tatgacaact tgacggctac 3061 atcattcact ttttcttcac aaccggcacg gaactcgctc gggctggccc cggtgcattt 3121 tttaaatacc cgcgagaaat agagttgatc gtcaaaacca acattgcgac cgacggtggc 3181 gataggcatc cgggtggtgc tcaaaagcag cttcgcctgg ctgatacgtt ggtcctcgcg 3241 ccagcttaag acgctaatcc ctaactgctg gcggaaaaga tgtgacagac gcgacggcga 3301 caagcaaaca tgctgtgcga cgctggcgat atcaaaattg ctgtctgcca ggtgatcgct 3361 gatgtactga caagcctcgc gtacccgatt atccatcggt ggatggagcg actcgttaat 3421 cgcttccatg cgccgcagta acaattgctc aagcagattt atcgccagca gctccgaata 3481 gcgcccttcc ccttgcccgg cgttaatgat ttgcccaaac aggtcgctga aatgcggctg 3541 gtgcgcttca tccgggcgaa agaaccccgt attggcaaat attgacggcc agttaagcca 3601 ttcatgccag taggcgcgcg gacgaaagta aacccactgg tgataccatt cgcgagcctc 3661 cggatgacga ccgtagtgat gaatctctcc tggcgggaac agcaaaatat cacccggtcg 3721 gcaaacaaat tctcgtccct gatttttcac caccccctga ccgcgaatgg tgagattgag 3781 aatataacct ttcattccca gcggtcggtc gataaaaaaa tcgagataac cgttggcctc 3841 aatcggcgtt aaacccgcca ccagatgggc attaaacgag tatcccggca gcaggggatc 3901 attttgcgct tcagccatac ttttcatact cccgccattc agagaagaaa ccaattgtcc 3961 atattgcatc agacattgcc gtcactgcgt cttttactgg ctcttctcgc taaccaaacc 4021 ggtaaccccg cttattaaaa gcattctgta acaaagcggg accaaagcca tgacaaaaac 4081 gcgtaacaaa agtgtctata atcacggcag aaaagtccac attgattatt tgcacggcgt 4141 cacactttgc tatgccatag catttttatc cataagatta gcggatccta cctgacgctt 4201 tttatcgcaa ctctctactg tttctccata cccgtttttt tgggctagct actagagtca 4261 cacaggaaag tactagatga aaaacataaa tgccgacgac acatacagaa taattaataa 4321 aattaaagct tgtagaagca ataatgatat taatcaatgc ttatctgata tgactaaaat 4381 ggtacattgt gaatattatt tactcgcgat catttatcct cattctatgg ttaaatctga 4441 tatttcaatc ctagataatt accctaaaaa atggaggcaa tattatgatg acgctaattt 4501 aataaaatat gatcctatag tagattattc taactccaat cattcaccaa ttaattggaa 4561 tatatttgaa aacaatgctg taaataaaaa atctccaaat gtaattaaag aagcgaaaac 4621 atcaggtctt atcactgggt ttagtttccc tattcatacg gctaacaatg gcttcggaat 4681 gcttagtttt gcacattcag aaaaagacaa ctatatagat agtttatttt tacatgcgtg 4741 tatgaacata ccattaattg ttccttctct agttgataat tatcgaaaaa taaatatagc 4801 aaataataaa tcaaacaacg atttaaccaa aagagaaaaa gaatgtttag cgtgggcatg 4861 cgaaggaaaa agctcttggg atatttcaaa aatattaggt tgcagtgagc gtactgtcac 4921 tttccattta accaatgcgc aaatgaaact caatacaaca aaccgctgcc aaagtatttc 4981 taaagcaatt ttaacaggag caattgattg cccatacttt aaaaattaat aacactgata 5041 gtgctagtgt agatcactac tagagtcaca caggaaagta ctagatggct tcctccgaag 5101 acgttatcaa agagttcatg cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg 5161 agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga aggtacccag accgctaaac 5221 tgaaagttac caaaggtggt ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc 5281 agtacggttc caaagcttac gttaaacacc cggctgacat cccggactac ctgaaactgt 5341 ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt cgaagacggt ggtgttgtta 5401 ccgttaccca ggactcctcc ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg 5461 gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa aaccatgggt tgggaagctt 5521 ccaccgaacg tatgtacccg gaagacggtg ctctgaaagg tgaaatcaaa atgcgtctga 5581 aactgaaaga cggtggtcac tacgacgctg aagttaaaac cacctacatg gctaaaaaac 5641 cggttcagct gccgggtgct tacaaaaccg acatcaaact ggacatcacc tcccacaacg 5701 aagactacac catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc accggtgctt 5761 aataacgctg atagtgctag tgtagatcgc tactagagcc aggcatcaaa taaaacgaaa 5821 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 5881 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagtccct 5941 atcagtgata gagattgaca tccctatcag tgatagagat actgagcact actagagtca 6001 cacaggaaag tactagatga cagtaaagaa gctttatttc gtcccagcag gtcgttgtat 6061 gttggatcat tcgtctgtta atagtacatt aacaccagga gaattattag acttaccggt 6121 ttggtgttat cttttggaga ctgaagaagg acctatttta gtagatacag gtatgccaga 6181 aagtgcagtt aataatgaag gtctttttaa cggtacattt gtcgaagggc aggttttacc 6241 gaaaatgact gaagaagata gaatcgtgaa tattttaaaa cgggttggtt atgagccgga 6301 agaccttctt tatattatta gttctcactt gcattttgat catgcaggag gaaatggcgc 6361 ttttataaat acaccaatca ttgtacagcg tgctgaatat gaggcggcgc agcatagcga 6421 agaatatttg aaagaatgta tattgccgaa tttaaactac aaaatcattg aaggtgatta 6481 tgaagtcgta ccaggagttc aattattgca tacaccaggc catactccag ggcatcaatc 6541 gctattaatt gagacagaaa aatccggtcc tgtattatta acgattgatg catcgtatac 6601 gaaagagaat tttgaaaatg aagtgccatt tgcgggattt gattcagaat tagctttatc 6661 ttcaattaaa cgtttaaaag aagtggtgat gaaagagaag ccgattgttt tctttggaca 6721 tgatatagag caggaaaggg gatgtaaagt gttccctgaa tatatagctg caaacgacga 6781 aaactacgct ttagtagctt aataacgctg atagtgctag tgtagatcgc tactagagcc 6841 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 6901 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 6961 gtttata //