The Lac Y and mRFP gene under the RecA(SOS) promoter in plasmid.
The Lac Y and mRFP gene under the RecA(SOS) promoter has been inserted in plasmid pSB2K3 (part number BBa_I0500).
The Lac Y and mRFP gene under the RecA(SOS) promoter.
The Lac Y gene has been put under the RecA(SOS) promoter.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
Campbell et al., PNAS v99 p7877 URL
monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
The Rec A(SOS) promoter has been PCR amplified from the chromosomal DNA from MG1655(Ecoli).
The Lac Y gene has been PCR amplified from the chromosomal DNA of MG1655 (Escherechia coli)and has been inserted into plasmid pSB1A2( Part number BBa_B0015).
Conversion of the iGEM parts registry to SBOL2.1