##gff-version 3
##sequence-region BBa_J22211 1 1685
BBa_J22211	.	engineered_region	1	799	.	+	0	ID=BBa_S01640;Name=BBa_S01640
BBa_J22211	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S01640
BBa_J22211	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J22211	.	CDS	19	774	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_S01640
BBa_J22211	.	engineered_region	19	774	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_J22211	.	CDS	19	768	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_J22211	.	engineered_region	19	20	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_J22211	.	point_mutation	192	192	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_J22211	.	engineered_tag	775	799	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_J22211	.	point_mutation	510	510	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_J22211	.	engineered_region	808	1685	.	+	0	ID=BBa_E0430;Name=BBa_E0430
BBa_J22211	.	terminator	1557	1636	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0430
BBa_J22211	.	stem_loop	1568	1611	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J22211	.	engineered_region	1557	1636	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J22211	.	terminator	1645	1685	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0430
BBa_J22211	.	stop_codon	1678	1678	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J22211	.	engineered_region	1645	1685	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J22211	.	stem_loop	1652	1671	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J22211	.	polyA_site	1672	1685	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J22211	.	ribosome_entry_site	808	819	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0430
BBa_J22211	.	conserved_region	812	815	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J22211	.	CDS	826	1548	.	+	0	ID=BBa_E0030;Name=BBa_E0030;Parent=BBa_E0430
>BBa_J22211
aaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaa
gcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcattta
tcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgct
aatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaaca
atgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctat
tcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttattttta
catgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaat
caaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaa
aatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgc
caaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagt
gtagatcactactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccat
cctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggc
aagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacg
gcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgt
ccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctg
gtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactaca
acagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcga
ggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaac
cactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtga
ccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaagg
ctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc
ttcgggtgggcctttctgcgtttata