LOCUS       BBa_J22241              1551 bp    DNA     linear       26-Dec-2024
DEFINITION  pTet rbs LuxI rbs CFP term
ACCESSION   BBa_J22241
VERSION     BBa_J22241.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..665
                     /label=LuxI
     CDS             81..665
                     /label=BBa_C0161
     misc_feature    678..681
                     /label=conserved
     RBS             674..685
                     /label=BBa_B0034
     CDS             692..1414
                     /label=BBa_E0020
     stem_loop       1434..1477
                     /label=stem_loop
     misc_feature    1423..1502
                     /label=BBa_B0010
     terminator      1423..1502
                     /label=BBa_B0010
     stem_loop       1518..1537
                     /label=T7 TE
     misc_feature    1544..1544
                     /label=stop
     polyA_site      1538..1551
                     /label=polya
     misc_feature    1511..1551
                     /label=BBa_B0012
     terminator      1511..1551
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgactataa tgataaaaaa atcggatttt ttggcaattc
      121 catcggagga gtataaaggt attctaagtc ttcgttatca agtgtttaag caaagacttg
      181 agtgggactt agttgtagaa aataaccttg aatcagatga gtatgataac tcaaatgcag
      241 aatatattta tgcttgtgat gatactgaaa atgtaagtgg atgctggcgt ttattaccta
      301 caacaggtga ttatatgctg aaaagtgttt ttcctgaatt gcttggtcaa cagagtgctc
      361 ccaaagatcc taatatagtc gaattaagtc gttttgctgt aggtaaaaat agctcaaaga
      421 taaataactc tgctagtgaa attacaatga aactatttga agctatatat aaacacgctg
      481 ttagtcaagg tattacagaa tatgtaacag taacatcaac agcaatagag cgatttttaa
      541 agcgtattaa agttccttgt catcgtattg gagacaaaga aattcatgta ttaggtgata
      601 ctaaatcggt tgtattgtct atgcctatta atgaacagtt taaaaaagca gtcttaaatt
      661 aataatacta gagaaagagg agaaatacta gatggtgagc aagggcgagg agctgttcac
      721 cggggtggtg cccatcctgg tcgagctgga cggcgacgtg aacggccaca agttcagcgt
      781 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      841 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca
      901 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      961 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     1021 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     1081 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca tcagccacaa
     1141 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca
     1201 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     1261 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     1321 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     1381 cactctcggc atggacgagc tgtacaagta ataatactag agccaggcat caaataaaac
     1441 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     1501 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//