LOCUS BBa_J22261 1748 bp DNA linear 31-Oct-2024 DEFINITION pLac rbs LuxR rbs YFP term ACCESSION BBa_J22261 VERSION BBa_J22261.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 82..83 /label=prefix misc_feature 255..255 /label=T misc_feature 573..573 /label=A CDS 82..831 /label=luxR misc_feature 82..837 /label=BBa_C0062 misc_feature 838..862 /label=Help:Barcodes CDS 82..837 /label=BBa_C0062 misc_feature 64..862 /label=BBa_S01640 misc_feature 875..878 /label=conserved RBS 871..882 /label=BBa_B0034 CDS 889..1611 /label=BBa_E0030 stem_loop 1631..1674 /label=stem_loop misc_feature 1620..1699 /label=BBa_B0010 terminator 1620..1699 /label=BBa_B0010 stem_loop 1715..1734 /label=T7 TE misc_feature 1741..1741 /label=stop polyA_site 1735..1748 /label=polya misc_feature 1708..1748 /label=BBa_B0012 terminator 1708..1748 /label=BBa_B0012 misc_feature 871..1748 /label=BBa_E0430 misc_feature 64..1748 /label=BBa_J22211 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt 121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact 181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa 241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct 301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat 361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg 421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc 481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat 541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat 601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg 661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact 721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt 781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac 841 tgatagtgct agtgtagatc actactagag aaagaggaga aatactagat ggtgagcaag 901 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac 961 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc 1021 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc 1081 ttcggctacg gcctgcaatg cttcgcccgc taccccgacc acatgaagct gcacgacttc 1141 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac 1201 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc 1261 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac 1321 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg 1381 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag 1441 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac 1501 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc 1561 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaata atactagagc 1621 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 1681 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 1741 cgtttata //