##gff-version 3
##sequence-region BBa_J22281 1 2697
BBa_J22281	.	engineered_region	1757	2697	.	+	0	ID=BBa_J22221;Name=BBa_J22221
BBa_J22281	.	promoter	1757	1811	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_J22221
BBa_J22281	.	non_covalent_binding_site	1757	1776	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J22281	.	promoter	1776	1781	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J22281	.	promoter	1798	1803	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J22281	.	start_codon	1809	1809	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J22281	.	engineered_region	1757	1811	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J22281	.	terminator	2569	2648	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J22221
BBa_J22281	.	stem_loop	2580	2623	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J22281	.	engineered_region	2569	2648	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J22281	.	ribosome_entry_site	1820	1831	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J22221
BBa_J22281	.	conserved_region	1824	1827	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J22281	.	terminator	2657	2697	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J22221
BBa_J22281	.	polyA_site	2684	2697	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J22281	.	stop_codon	2690	2690	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J22281	.	engineered_region	2657	2697	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J22281	.	stem_loop	2664	2683	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J22281	.	CDS	1838	2560	.	+	0	ID=BBa_E0020;Name=BBa_E0020;Parent=BBa_J22221
BBa_J22281	.	engineered_region	1	1748	.	+	0	ID=BBa_J22261;Name=BBa_J22261
BBa_J22281	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_J22261
BBa_J22281	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J22281	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J22281	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J22281	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J22281	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J22281	.	engineered_region	64	1748	.	+	0	ID=BBa_J22211;Name=BBa_J22211;Parent=BBa_J22261
BBa_J22281	.	engineered_region	64	862	.	+	0	ID=BBa_S01640;Name=BBa_S01640;Parent=BBa_J22211
BBa_J22281	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S01640
BBa_J22281	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J22281	.	CDS	82	837	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_S01640
BBa_J22281	.	CDS	82	831	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_J22281	.	engineered_region	82	837	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_J22281	.	engineered_region	82	83	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_J22281	.	point_mutation	255	255	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_J22281	.	engineered_tag	838	862	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_J22281	.	point_mutation	573	573	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_J22281	.	engineered_region	871	1748	.	+	0	ID=BBa_E0430;Name=BBa_E0430;Parent=BBa_J22211
BBa_J22281	.	terminator	1620	1699	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0430
BBa_J22281	.	stem_loop	1631	1674	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J22281	.	engineered_region	1620	1699	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J22281	.	ribosome_entry_site	871	882	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0430
BBa_J22281	.	conserved_region	875	878	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J22281	.	terminator	1708	1748	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0430
BBa_J22281	.	polyA_site	1735	1748	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J22281	.	stop_codon	1741	1741	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J22281	.	engineered_region	1708	1748	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J22281	.	stem_loop	1715	1734	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J22281	.	CDS	889	1611	.	+	0	ID=BBa_E0030;Name=BBa_E0030;Parent=BBa_E0430
>BBa_J22281
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaa
tcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtt
aaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatg
atcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaa
atctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaat
ggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaaca
taccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaac
caaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagt
gagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaag
caattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactaga
gaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggac
ggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagt
tcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgc
ccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatc
ttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagc
tgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtcta
tatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcag
ctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctacc
agtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcac
tctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactg
ggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttc
tgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactag
agaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctgga
cggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaag
ttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttca
gccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccat
cttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgag
ctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtct
atatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgca
gctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacc
cagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatca
ctctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagact
gggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggccttt
ctgcgtttata