LOCUS BBa_J22291 3308 bp DNA linear 22-Dec-2024 DEFINITION J22231 J22271 ACCESSION BBa_J22291 VERSION BBa_J22291.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 82..666 /label=LuxI CDS 82..666 /label=BBa_C0161 misc_feature 679..682 /label=conserved RBS 675..686 /label=BBa_B0034 CDS 693..1415 /label=BBa_E0020 stem_loop 1435..1478 /label=stem_loop misc_feature 1424..1503 /label=BBa_B0010 terminator 1424..1503 /label=BBa_B0010 stem_loop 1519..1538 /label=T7 TE misc_feature 1545..1545 /label=stop polyA_site 1539..1552 /label=polya misc_feature 1512..1552 /label=BBa_B0012 terminator 1512..1552 /label=BBa_B0012 misc_feature 1..1552 /label=BBa_J22231 misc_feature 1561..1580 /label=LuxR/HSL promoter 1580..1585 /label=-35 promoter 1602..1607 /label=-10 misc_feature 1613..1613 /label=start misc_feature 1561..1615 /label=BBa_R0062 promoter 1561..1615 /label=BBa_R0062 misc_feature 1628..1631 /label=conserved RBS 1624..1635 /label=BBa_B0034 misc_feature 1642..1643 /label=prefix misc_feature 1815..1815 /label=T misc_feature 2133..2133 /label=A CDS 1642..2391 /label=luxR misc_feature 1642..2397 /label=BBa_C0062 misc_feature 2398..2422 /label=Help:Barcodes CDS 1642..2397 /label=BBa_C0062 misc_feature 1624..2422 /label=BBa_S01640 misc_feature 2435..2438 /label=conserved RBS 2431..2442 /label=BBa_B0034 CDS 2449..3171 /label=BBa_E0030 stem_loop 3191..3234 /label=stem_loop misc_feature 3180..3259 /label=BBa_B0010 terminator 3180..3259 /label=BBa_B0010 stem_loop 3275..3294 /label=T7 TE misc_feature 3301..3301 /label=stop polyA_site 3295..3308 /label=polya misc_feature 3268..3308 /label=BBa_B0012 terminator 3268..3308 /label=BBa_B0012 misc_feature 2431..3308 /label=BBa_E0430 misc_feature 1624..3308 /label=BBa_J22211 misc_feature 1561..3308 /label=BBa_J22271 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgactata atgataaaaa aatcggattt tttggcaatt 121 ccatcggagg agtataaagg tattctaagt cttcgttatc aagtgtttaa gcaaagactt 181 gagtgggact tagttgtaga aaataacctt gaatcagatg agtatgataa ctcaaatgca 241 gaatatattt atgcttgtga tgatactgaa aatgtaagtg gatgctggcg tttattacct 301 acaacaggtg attatatgct gaaaagtgtt tttcctgaat tgcttggtca acagagtgct 361 cccaaagatc ctaatatagt cgaattaagt cgttttgctg taggtaaaaa tagctcaaag 421 ataaataact ctgctagtga aattacaatg aaactatttg aagctatata taaacacgct 481 gttagtcaag gtattacaga atatgtaaca gtaacatcaa cagcaataga gcgattttta 541 aagcgtatta aagttccttg tcatcgtatt ggagacaaag aaattcatgt attaggtgat 601 actaaatcgg ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat 661 taataatact agagaaagag gagaaatact agatggtgag caagggcgag gagctgttca 721 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt gaacggccac aagttcagcg 781 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca 841 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tggggcgtgc 901 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc 961 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc 1021 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg 1081 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac atcagccaca 1141 acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc 1201 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg 1261 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca 1321 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga 1381 tcactctcgg catggacgag ctgtacaagt aataatacta gagccaggca tcaaataaaa 1441 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct 1501 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag 1561 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta 1621 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt 1681 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact 1741 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa 1801 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct 1861 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat 1921 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg 1981 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc 2041 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat 2101 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat 2161 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg 2221 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact 2281 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt 2341 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac 2401 tgatagtgct agtgtagatc actactagag aaagaggaga aatactagat ggtgagcaag 2461 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac 2521 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc 2581 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc 2641 ttcggctacg gcctgcaatg cttcgcccgc taccccgacc acatgaagct gcacgacttc 2701 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac 2761 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc 2821 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac 2881 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg 2941 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag 3001 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac 3061 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc 3121 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaata atactagagc 3181 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 3241 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 3301 cgtttata //