##gff-version 3
##sequence-region BBa_J22291 1 3308
BBa_J22291	.	engineered_region	1561	3308	.	+	0	ID=BBa_J22271;Name=BBa_J22271
BBa_J22291	.	engineered_region	1624	3308	.	+	0	ID=BBa_J22211;Name=BBa_J22211;Parent=BBa_J22271
BBa_J22291	.	engineered_region	2431	3308	.	+	0	ID=BBa_E0430;Name=BBa_E0430;Parent=BBa_J22211
BBa_J22291	.	ribosome_entry_site	2431	2442	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0430
BBa_J22291	.	conserved_region	2435	2438	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J22291	.	CDS	2449	3171	.	+	0	ID=BBa_E0030;Name=BBa_E0030;Parent=BBa_E0430
BBa_J22291	.	terminator	3180	3259	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0430
BBa_J22291	.	engineered_region	3180	3259	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J22291	.	stem_loop	3191	3234	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J22291	.	terminator	3268	3308	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0430
BBa_J22291	.	engineered_region	3268	3308	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J22291	.	stop_codon	3301	3301	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J22291	.	polyA_site	3295	3308	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J22291	.	stem_loop	3275	3294	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J22291	.	engineered_region	1624	2422	.	+	0	ID=BBa_S01640;Name=BBa_S01640;Parent=BBa_J22211
BBa_J22291	.	ribosome_entry_site	1624	1635	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S01640
BBa_J22291	.	conserved_region	1628	1631	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J22291	.	CDS	1642	2397	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_S01640
BBa_J22291	.	point_mutation	2133	2133	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_J22291	.	point_mutation	1815	1815	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_J22291	.	CDS	1642	2391	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_J22291	.	engineered_region	1642	1643	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_J22291	.	engineered_region	1642	2397	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_J22291	.	engineered_tag	2398	2422	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_J22291	.	promoter	1561	1615	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_J22271
BBa_J22291	.	non_covalent_binding_site	1561	1580	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J22291	.	promoter	1602	1607	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J22291	.	start_codon	1613	1613	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J22291	.	promoter	1580	1585	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J22291	.	engineered_region	1561	1615	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J22291	.	engineered_region	1	1552	.	+	0	ID=BBa_J22231;Name=BBa_J22231
BBa_J22291	.	terminator	1512	1552	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J22231
BBa_J22291	.	engineered_region	1512	1552	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J22291	.	stop_codon	1545	1545	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J22291	.	polyA_site	1539	1552	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J22291	.	stem_loop	1519	1538	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J22291	.	ribosome_entry_site	675	686	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J22231
BBa_J22291	.	conserved_region	679	682	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J22291	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_J22231
BBa_J22291	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J22291	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J22291	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J22291	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J22291	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J22291	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J22231
BBa_J22291	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J22291	.	terminator	1424	1503	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J22231
BBa_J22291	.	engineered_region	1424	1503	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J22291	.	stem_loop	1435	1478	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J22291	.	CDS	693	1415	.	+	0	ID=BBa_E0020;Name=BBa_E0020;Parent=BBa_J22231
BBa_J22291	.	CDS	82	666	.	+	0	ID=BBa_C0161;Name=BBa_C0161;Parent=BBa_J22231
BBa_J22291	.	CDS	82	666	.	+	0	ID=annotation1891615;Name=LuxI;Parent=BBa_C0161
>BBa_J22291
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgtta
tcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaat
gcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattata
tgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttt
tgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaa
cacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaag
ttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaa
tgaacagtttaaaaaagcagtcttaaattaataatactagagaaagaggagaaatactagatggtgagcaagggcgagg
agctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcga
gggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggccc
accctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttca
agtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccga
ggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctg
gggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggcca
acttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcga
cggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgat
cacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggt
ttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatgaaaaacataaatgcc
gacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatga
ctaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcct
agataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattct
aactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaag
aagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttt
tgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttct
ctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtt
tagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttcca
tttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaatt
gattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactaga
tggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaa
gttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaag
ctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatga
agctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaa
ctacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaag
gaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcaga
agaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagca
gaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagac
cccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgt
acaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata