LOCUS BBa_J22301 3308 bp DNA linear 22-Dec-2024 DEFINITION J22261 J22251 ACCESSION BBa_J22301 VERSION BBa_J22301.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 82..83 /label=prefix misc_feature 255..255 /label=T misc_feature 573..573 /label=A CDS 82..831 /label=luxR misc_feature 82..837 /label=BBa_C0062 misc_feature 838..862 /label=Help:Barcodes CDS 82..837 /label=BBa_C0062 misc_feature 64..862 /label=BBa_S01640 misc_feature 875..878 /label=conserved RBS 871..882 /label=BBa_B0034 CDS 889..1611 /label=BBa_E0030 stem_loop 1631..1674 /label=stem_loop misc_feature 1620..1699 /label=BBa_B0010 terminator 1620..1699 /label=BBa_B0010 stem_loop 1715..1734 /label=T7 TE misc_feature 1741..1741 /label=stop polyA_site 1735..1748 /label=polya misc_feature 1708..1748 /label=BBa_B0012 terminator 1708..1748 /label=BBa_B0012 misc_feature 871..1748 /label=BBa_E0430 misc_feature 64..1748 /label=BBa_J22211 misc_feature 1..1748 /label=BBa_J22261 misc_feature 1757..1776 /label=LuxR/HSL promoter 1776..1781 /label=-35 promoter 1798..1803 /label=-10 misc_feature 1809..1809 /label=start misc_feature 1757..1811 /label=BBa_R0062 promoter 1757..1811 /label=BBa_R0062 misc_feature 1824..1827 /label=conserved RBS 1820..1831 /label=BBa_B0034 CDS 1838..2422 /label=LuxI CDS 1838..2422 /label=BBa_C0161 misc_feature 2435..2438 /label=conserved RBS 2431..2442 /label=BBa_B0034 CDS 2449..3171 /label=BBa_E0020 stem_loop 3191..3234 /label=stem_loop misc_feature 3180..3259 /label=BBa_B0010 terminator 3180..3259 /label=BBa_B0010 stem_loop 3275..3294 /label=T7 TE misc_feature 3301..3301 /label=stop polyA_site 3295..3308 /label=polya misc_feature 3268..3308 /label=BBa_B0012 terminator 3268..3308 /label=BBa_B0012 misc_feature 1757..3308 /label=BBa_J22251 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt 121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact 181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa 241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct 301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat 361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg 421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc 481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat 541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat 601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg 661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact 721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt 781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac 841 tgatagtgct agtgtagatc actactagag aaagaggaga aatactagat ggtgagcaag 901 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac 961 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc 1021 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc 1081 ttcggctacg gcctgcaatg cttcgcccgc taccccgacc acatgaagct gcacgacttc 1141 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac 1201 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc 1261 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac 1321 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg 1381 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag 1441 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac 1501 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc 1561 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaata atactagagc 1621 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 1681 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 1741 cgtttatata ctagagacct gtaggatcgt acaggtttac gcaagaaaat ggtttgttat 1801 agtcgaataa atactagaga aagaggagaa atactagatg actataatga taaaaaaatc 1861 ggattttttg gcaattccat cggaggagta taaaggtatt ctaagtcttc gttatcaagt 1921 gtttaagcaa agacttgagt gggacttagt tgtagaaaat aaccttgaat cagatgagta 1981 tgataactca aatgcagaat atatttatgc ttgtgatgat actgaaaatg taagtggatg 2041 ctggcgttta ttacctacaa caggtgatta tatgctgaaa agtgtttttc ctgaattgct 2101 tggtcaacag agtgctccca aagatcctaa tatagtcgaa ttaagtcgtt ttgctgtagg 2161 taaaaatagc tcaaagataa ataactctgc tagtgaaatt acaatgaaac tatttgaagc 2221 tatatataaa cacgctgtta gtcaaggtat tacagaatat gtaacagtaa catcaacagc 2281 aatagagcga tttttaaagc gtattaaagt tccttgtcat cgtattggag acaaagaaat 2341 tcatgtatta ggtgatacta aatcggttgt attgtctatg cctattaatg aacagtttaa 2401 aaaagcagtc ttaaattaat aatactagag aaagaggaga aatactagat ggtgagcaag 2461 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtgaac 2521 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc 2581 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc 2641 ctgacctggg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc 2701 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac 2761 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc 2821 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac 2881 aactacatca gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc 2941 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag 3001 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc 3061 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc 3121 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaata atactagagc 3181 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 3241 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 3301 cgtttata //