##gff-version 3 ##sequence-region BBa_J22301 1 3308 BBa_J22301 . engineered_region 1757 3308 . + 0 ID=BBa_J22251;Name=BBa_J22251 BBa_J22301 . terminator 3268 3308 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J22251 BBa_J22301 . stem_loop 3275 3294 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_J22301 . engineered_region 3268 3308 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_J22301 . stop_codon 3301 3301 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_J22301 . polyA_site 3295 3308 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_J22301 . terminator 3180 3259 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J22251 BBa_J22301 . stem_loop 3191 3234 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_J22301 . engineered_region 3180 3259 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_J22301 . promoter 1757 1811 . + 0 ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_J22251 BBa_J22301 . promoter 1776 1781 . + 0 ID=annotation2046;Name=-35;Parent=BBa_R0062 BBa_J22301 . non_covalent_binding_site 1757 1776 . + 0 ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062 BBa_J22301 . engineered_region 1757 1811 . + 0 ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062 BBa_J22301 . start_codon 1809 1809 . + 0 ID=annotation2048;Name=start;Parent=BBa_R0062 BBa_J22301 . promoter 1798 1803 . + 0 ID=annotation2047;Name=-10;Parent=BBa_R0062 BBa_J22301 . ribosome_entry_site 1820 1831 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J22251 BBa_J22301 . conserved_region 1824 1827 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_J22301 . ribosome_entry_site 2431 2442 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J22251 BBa_J22301 . conserved_region 2435 2438 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_J22301 . CDS 2449 3171 . + 0 ID=BBa_E0020;Name=BBa_E0020;Parent=BBa_J22251 BBa_J22301 . CDS 1838 2422 . + 0 ID=BBa_C0161;Name=BBa_C0161;Parent=BBa_J22251 BBa_J22301 . CDS 1838 2422 . + 0 ID=annotation1891615;Name=LuxI;Parent=BBa_C0161 BBa_J22301 . engineered_region 1 1748 . + 0 ID=BBa_J22261;Name=BBa_J22261 BBa_J22301 . engineered_region 64 1748 . + 0 ID=BBa_J22211;Name=BBa_J22211;Parent=BBa_J22261 BBa_J22301 . engineered_region 871 1748 . + 0 ID=BBa_E0430;Name=BBa_E0430;Parent=BBa_J22211 BBa_J22301 . CDS 889 1611 . + 0 ID=BBa_E0030;Name=BBa_E0030;Parent=BBa_E0430 BBa_J22301 . terminator 1708 1748 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0430 BBa_J22301 . stem_loop 1715 1734 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_J22301 . engineered_region 1708 1748 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_J22301 . stop_codon 1741 1741 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_J22301 . polyA_site 1735 1748 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_J22301 . ribosome_entry_site 871 882 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0430 BBa_J22301 . conserved_region 875 878 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_J22301 . terminator 1620 1699 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0430 BBa_J22301 . stem_loop 1631 1674 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_J22301 . engineered_region 1620 1699 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_J22301 . engineered_region 64 862 . + 0 ID=BBa_S01640;Name=BBa_S01640;Parent=BBa_J22211 BBa_J22301 . ribosome_entry_site 64 75 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S01640 BBa_J22301 . conserved_region 68 71 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_J22301 . CDS 82 837 . + 0 ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_S01640 BBa_J22301 . CDS 82 831 . + 0 ID=annotation1766;Name=luxR;Parent=BBa_C0062 BBa_J22301 . engineered_region 82 837 . + 0 ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062 BBa_J22301 . engineered_region 82 83 . + 0 ID=annotation1762;Name=prefix;Parent=BBa_C0062 BBa_J22301 . point_mutation 255 255 . + 0 ID=annotation1764;Name=T;Parent=BBa_C0062 BBa_J22301 . engineered_tag 838 862 . + 0 ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062 BBa_J22301 . point_mutation 573 573 . + 0 ID=annotation1765;Name=A;Parent=BBa_C0062 BBa_J22301 . promoter 1 54 . + 0 ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_J22261 BBa_J22301 . operator 26 42 . + 0 ID=annotation2001;Name=lac O1;Parent=BBa_R0011 BBa_J22301 . operator 3 19 . + 0 ID=annotation1999;Name=lac O1;Parent=BBa_R0011 BBa_J22301 . promoter 20 25 . + 0 ID=annotation2000;Name=-35;Parent=BBa_R0011 BBa_J22301 . promoter 43 48 . + 0 ID=annotation2002;Name=-10;Parent=BBa_R0011 BBa_J22301 . engineered_region 1 54 . + 0 ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011 >BBa_J22301 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact agatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaa tcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtt aaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatg atcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaa atctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaat ggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaaca taccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaac caaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagt gagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaag caattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactaga gaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggac ggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagt tcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgc ccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatc ttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagc tgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtcta tatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcag ctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctacc agtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcac tctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactg ggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttc tgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactag agaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaagg tattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagat gagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattac ctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatat agtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaacta tttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgat ttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgt attgtctatgcctattaatgaacagtttaaaaaagcagtcttaaattaataatactagagaaagaggagaaatactaga tggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaa gttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaag ctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatga agcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaa ctacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaag gaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcaga agaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagca gaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagac cccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgt acaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata