LOCUS BBa_J23004 3090 bp DNA linear 23-Dec-2024 DEFINITION Plasmid for destroying biobrick restriction sites ACCESSION BBa_J23004 VERSION BBa_J23004.1 FEATURES Location/Qualifiers misc_feature 33..955 /label=OnRFP misc_feature 1213..1895 /label=ColE1 origin CDS 1993..2652 /label=Bla ORIGIN 1 gaattcgcgg ccgcaactag tgtccctatc agtccctatc agtgatagag attgacatcc 61 ctatcagtga tagagatact gagcactact agagaaagag gagaaatact agatggcttc 121 ctccgaagac gttatcaaag agttcatgcg tttcaaagtt cgtatggaag gttccgttaa 181 cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac 241 cgctaaactg aaagttacca aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc 301 gcagttccag tacggttcca aagcttacgt taaacacccg gctgacatcc cggactacct 361 gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg 421 tgttgttacc gttacccagg actcctccct gcaagacggt gagttcatct acaaagttaa 481 actgcgtggt accaacttcc cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg 541 ggaagcttcc accgaacgta tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat 601 gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca cctacatggc 661 taaaaaaccg gttcagctgc cgggtgctta caaaaccgac atcaaactgg acatcacctc 721 ccacaacgaa gactacacca tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac 781 cggtgcttaa taacgctgat agtgctagtg tagatcgcta ctagagccag gcatcaaata 841 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 901 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatattcta 961 gaagcggccg ctgcagtccg gcaaaaaaac gggcaaggtg tcaccaccct gccctttttc 1021 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg 1081 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 1141 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 1201 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 1261 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 1321 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 1381 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 1441 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 1501 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 1561 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 1621 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 1681 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 1741 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 1801 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 1861 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 1921 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 1981 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 2041 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 2101 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 2161 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 2221 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 2281 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 2341 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 2401 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 2461 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 2521 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 2581 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 2641 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 2701 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 2761 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 2821 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 2881 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 2941 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 3001 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa 3061 atccttagct ttcgctaagg atgatttctg //