LOCUS       BBa_J23010              1316 bp    DNA     linear       25-Nov-2024
DEFINITION  [OriTr]+[OnGFP]
ACCESSION   BBa_J23010
VERSION     BBa_J23010.1  
FEATURES             Location/Qualifiers
     oriT            1..371
                     /label=BBa_J01003
     misc_feature    380..398
                     /label=TetR 1
     misc_feature    380..433
                     /label=BBa_R0040
     promoter        399..404
                     /label=-35
     misc_feature    405..423
                     /label=TetR 2
     promoter        422..427
                     /label=-10
     promoter        380..433
                     /label=BBa_R0040
     misc_feature    446..449
                     /label=conserved
     RBS             442..453
                     /label=BBa_B0034
     CDS             460..1179
                     /label=GFP protein
     CDS             460..1179
                     /label=BBa_E0040
     stem_loop       1199..1242
                     /label=stem_loop
     misc_feature    1188..1267
                     /label=BBa_B0010
     terminator      1188..1267
                     /label=BBa_B0010
     stem_loop       1283..1302
                     /label=T7 TE
     misc_feature    1309..1309
                     /label=stop
     polyA_site      1303..1316
                     /label=polya
     misc_feature    1276..1316
                     /label=BBa_B0012
     terminator      1276..1316
                     /label=BBa_B0012
ORIGIN
        1 gccgcctttt cctcaatcgc tcttcgttcg tctggaaggc agtacacctt gataggtggg
       61 ctgcccttcc tggttggctt ggtttcatca gccatccgct tgccctcatc tgttacgccg
      121 gcggtagccg gccagcctcg cagagcagga ttcccgttga gcaccgccag gtgcgaataa
      181 gggacagtga agaaggaaca cccgctcgcg ggtgggccta cttcacctat cctgcccggc
      241 tgacgccgtt ggatacacca aggaaagtct acacgaaccc tttggcaaaa tcctgtatat
      301 cgtgcgaaaa aggatggata taccgaaaaa atcgctataa tgaccccgaa gcagggttat
      361 gcagcggaaa atactagagt ccctatcagt gatagagatt gacatcccta tcagtgatag
      421 agatactgag cactactaga gaaagaggag aaatactaga tgcgtaaagg agaagaactt
      481 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt
      541 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt
      601 tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt
      661 gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc
      721 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag
      781 acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt
      841 attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca
      901 cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt
      961 agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca
     1021 attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt
     1081 tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct
     1141 gggattacac atggcatgga tgaactatac aaataataat actagagcca ggcatcaaat
     1201 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     1261 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//