##gff-version 3
##sequence-region BBa_J23010 1 1316
BBa_J23010	.	terminator	1276	1316	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J23010	.	engineered_region	1276	1316	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J23010	.	stop_codon	1309	1309	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J23010	.	stem_loop	1283	1302	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J23010	.	polyA_site	1303	1316	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J23010	.	CDS	460	1179	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_J23010	.	CDS	460	1179	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J23010	.	ribosome_entry_site	442	453	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J23010	.	conserved_region	446	449	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J23010	.	promoter	380	433	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J23010	.	promoter	399	404	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J23010	.	non_covalent_binding_site	405	423	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J23010	.	engineered_region	380	433	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J23010	.	promoter	422	427	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J23010	.	non_covalent_binding_site	380	398	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J23010	.	oriT	1	371	.	+	0	ID=BBa_J01003;Name=BBa_J01003
BBa_J23010	.	terminator	1188	1267	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J23010	.	engineered_region	1188	1267	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J23010	.	stem_loop	1199	1242	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_J23010
gccgccttttcctcaatcgctcttcgttcgtctggaaggcagtacaccttgataggtgggctgcccttcctggttggct
tggtttcatcagccatccgcttgccctcatctgttacgccggcggtagccggccagcctcgcagagcaggattcccgtt
gagcaccgccaggtgcgaataagggacagtgaagaaggaacacccgctcgcgggtgggcctacttcacctatcctgccc
ggctgacgccgttggatacaccaaggaaagtctacacgaaccctttggcaaaatcctgtatatcgtgcgaaaaaggatg
gatataccgaaaaaatcgctataatgaccccgaagcagggttatgcagcggaaaatactagagtccctatcagtgatag
agattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgcgtaaaggagaa
gaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggag
agggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggcc
aacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttc
aagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctg
aagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattct
tggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagtt
aacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcg
atggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagaga
ccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactag
agccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgct
ctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata